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1. X-ray crystallography shines a light on pore-forming toxins
2. Giant unilamellar vesicles for studying activity of pore forming proteins
3. Combined applications of fluorescence spectroscopy and molecular dynamics simulations in studies of Diphteria Toxin translocation domain
Alexey S. Ladokhin
4. Biophysical approaches to study actinoporin-lipid interactions
5. Molecular basis for activation of Actinoporins by lipids
6. Engineered ClyA for detection of biological molecules
7. Pore-forming Toxins for the Size-Discrimination of Polymers and Biopolymers: Towards Biomolecules Sequencing
8. Use of Pore-Forming Toxins to study co-translocational protein folding
9. The use of pore-forming toxins to image lipids and lipid domains
10. Engineering outer membrane porin for protein detection
11. The use of Ostreolysin A and Anthrolysin O to study cholesterol on cell membranes
12. Studying the mechanism of Pore-forming Toxins using High-speed Atomic Force Microscopy
13. The use of Atomic-Force Microscopy to study Pore-Forming Toxins
14. Cysteine-based crosslinking approach for characterization of oligomeric pore-forming proteins in the mitochondrial membrane
15. High-resolution structure and the sample preparation of Clostridium perfringens iota-toxin pore complex
16. How protein engineering has revealed the molecular mechanisms of pore-forming toxins?
17. Single Molecule Fluorescence Techniques to Study the Mechanism of Pore-Forming Toxins
19. Going full circle: determining the structures of complement component
20. Different MD simulation techniques used to study Pore-forming Toxins
21. Contribution of Molecular Dynamics Simulations to the study of Pore Forming Toxins
22. Membranes with lipid asymmetry for studying Pore-Forming Toxins
23. Topological analysis of Pore-forming proteins in native membranes
Pore Forming Toxins, Volume 649 in the Methods in Enzymology series continues the legacy of this premier serial with quality chapters authored by field leaders. Chapters in this new release include X-ray crystallography shines a light on pore-forming toxins, Giant unilamellar vesicles for studying activity of pore forming proteins, Combined applications of fluorescence spectroscopy and molecular dynamics simulations in studies of Diphtheria Toxin translocation domain, Biophysical approaches to study actinoporin-lipid interactions, Molecular basis for activation of Actinoporins by lipids, Engineered ClyA for detection of biological molecules, Pore-forming Toxins for the Size-Discrimination of Polymers and Biopolymers: Towards Biomolecules Sequencing, and much more.
- Provides the authority and expertise of leading contributors from an international board of authors
- Presents the latest release in the Methods in Enzymology series
Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists
- No. of pages:
- © Academic Press 2021
- 1st March 2021
- Academic Press
- Hardcover ISBN:
Alejandro Heuck is at Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, USA
Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, USA
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