Pore-Forming Toxins

Pore-Forming Toxins

1st Edition - March 10, 2021
This is the Latest Edition
  • Editor: Alejandro Heuck
  • eBook ISBN: 9780128238592
  • Hardcover ISBN: 9780128238585

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Description

Pore Forming Toxins, Volume 649 in the Methods in Enzymology series continues the legacy of this premier serial with quality chapters authored by field leaders. Chapters in this new release include X-ray crystallography shines a light on pore-forming toxins, Giant unilamellar vesicles for studying activity of pore forming proteins, Combined applications of fluorescence spectroscopy and molecular dynamics simulations in studies of Diphtheria Toxin translocation domain, Biophysical approaches to study actinoporin-lipid interactions, Molecular basis for activation of Actinoporins by lipids, Engineered ClyA for detection of biological molecules, Pore-forming Toxins for the Size-Discrimination of Polymers and Biopolymers: Towards Biomolecules Sequencing, and much more.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

  • 1. X-ray crystallography shines a light on pore-forming toxins
    Michael Parker
    2. Giant unilamellar vesicles for studying activity of pore forming proteins
    Gregor Anderluh
    3. Combined applications of fluorescence spectroscopy and molecular dynamics simulations in studies of Diphteria Toxin translocation domain
    Alexey S. Ladokhin
    4. Biophysical approaches to study actinoporin-lipid interactions
    Sara Garcia-Linares
    5. Molecular basis for activation of Actinoporins by lipids
    Kouei Tsumoto
    6. Engineered ClyA for detection of biological molecules
    Giovanni Maglia
    7. Pore-forming Toxins for the Size-Discrimination of Polymers and Biopolymers: Towards Biomolecules Sequencing
    Abdelghani Oukhaled
    8. Use of Pore-Forming Toxins to study co-translocational protein folding
    David Rodriguez-Larrea
    9. The use of pore-forming toxins to image lipids and lipid domains
    Toshihide Kobayashi
    10. Engineering outer membrane porin for protein detection
    Min Chen
    11. The use of Ostreolysin A and Anthrolysin O to study cholesterol on cell membranes
    Arun Radhakrishnan
    12. Studying the mechanism of Pore-forming Toxins using High-speed Atomic Force Microscopy
    Simon Scheuring
    13. The use of Atomic-Force Microscopy to study Pore-Forming Toxins
    Bart Hoogenboom
    14. Cysteine-based crosslinking approach for characterization of oligomeric pore-forming proteins in the mitochondrial membrane
    Jialing Lin
    15. High-resolution structure and the sample preparation of Clostridium perfringens iota-toxin pore complex
    Hideaki Tsuge
    16. How protein engineering has revealed the molecular mechanisms of pore-forming toxins?
    Rodney Tweten
    17. Single Molecule Fluorescence Techniques to Study the Mechanism of Pore-Forming Toxins
    Mark Wallace
    18. Cryo-EM
    Robert Gilbert
    19. Going full circle: determining the structures of complement component
    Michelle Dunstone
    20. Different MD simulation techniques used to study Pore-forming Toxins
    Ganapathy Ayappa
    21. Contribution of Molecular Dynamics Simulations to the study of Pore Forming Toxins
    Gerhard Hummer
    22. Membranes with lipid asymmetry for studying Pore-Forming Toxins
    Erwin London
    23. Topological analysis of Pore-forming proteins in native membranes
    Alejandro Heuck

Product details

  • No. of pages: 672
  • Language: English
  • Copyright: © Academic Press 2021
  • Published: March 10, 2021
  • Imprint: Academic Press
  • eBook ISBN: 9780128238592
  • Hardcover ISBN: 9780128238585

About the Serial Volume Editor

Alejandro Heuck

Alejandro Heuck is at Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, USA

Affiliations and Expertise

Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, USA