Hydrogen Sulfide in Redox Biology Part B

Hydrogen Sulfide in Redox Biology Part B

1st Edition - March 3, 2015

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  • Editors: Enrique Cadenas, Lester Packer
  • eBook ISBN: 9780128016220
  • Hardcover ISBN: 9780128015117

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These new volumes of Methods in Enzymology (554 and 555) on Hydrogen Sulfide Signaling continue the legacy established by previous volumes on another gasotransmitter, nitric oxide (Methods in Enzymology volumes 359, 396, 440, and 441), with quality chapters authored by leaders in the field of hydrogen sulfide research. These volumes of Methods in Enzymology were designed as a compendium for hydrogen sulfide detection methods, the pharmacological activity of hydrogen sulfide donors, the redox biochemistry of hydrogen sulfide and its metabolism in mammalian tissues, the mechanisms inherent in hydrogen sulfide cell signaling and transcriptional pathways, and cell signaling in specific systems, such as cardiovascular and nervous system as well as its function in inflammatory responses. Two chapters are also devoted to hydrogen sulfide in plants and a newcomer, molecular hydrogen, its function as a novel antioxidant.

Key Features

  • Continues the legacy of this premier serial with quality chapters on hydrogen sulfide research authored by leaders in the field
  • Covers conventional and new hydrogen sulfide detection methods
  • Covers the pharmacological activity of hydrogen sulfide donors
  • Contains chapters on important topics on hydrogen sulfide modulation of cell signaling and transcriptional pathways, and the role of hydrogen sulfide in the cardiovascular and nervous systems and in inflammation


Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists.

Table of Contents

    • Preface
    • Section I: The Redox Biochemistry of Hydrogen Sulfide
      • Chapter One: Investigating the Role of H2S in 4-HNE Scavenging
        • Abstract
        • 1 Introduction
        • 2 Experimental Compounds and Considerations
        • 3 Conclusions and Perspectives
        • Acknowledgment
      • Chapter Two: Inhalation Exposure Model of Hydrogen Sulfide (H2S)-Induced Hypometabolism in the Male Sprague-Dawley Rat
        • Abstract
        • 1 Introduction
        • 2 Exposure Protocol for H2S-Induced Hypometabolism in Rats
        • 3 Other Considerations for H2S Exposure Studies
        • 4 Summary
        • Acknowledgments
    • Section II: Mechanisms of H2S Cell Signaling and Transcriptional Pathways
      • Chapter Three: Use of the “Tag-Switch” Method for the Detection of Protein S-Sulfhydration
        • Abstract
        • 1 Introduction
        • 2 The Design of “Tag-Switch” Method
        • 3 Chemistry Validation Using Small-Molecule Substrates
        • 4 “Tag-Switch” Assay on Bovine Serum Albumin and GAPDH as Model Proteins
        • 5 “Tag-Switch” Assay for the Detection on Intracellular Protein Persulfides
        • 6 “Tag-Switch” Assay for the Detection of Intracellular S-Sulfhydration by Fluorescence Microscopy
        • 7 Conclusions
        • Acknowledgments
      • Chapter Four: Real-Time Assays for Monitoring the Influence of Sulfide and Sulfane Sulfur Species on Protein Thiol Redox States
        • Abstract
        • 1 Introduction
        • 2 PTEN Activity Assay
        • 3 roGFP2 Redox Assay
        • 4 Application of “H2S Donors” and Polysulfides
        • 5 Quantitation of Sulfane Sulfur by Cold Cyanolysis
        • 6 Elimination of Sulfane Sulfur by Cold Cyanolysis
        • Acknowledgments
      • Chapter Five: Protein Sulfhydration
        • Abstract
        • 1 Introduction
        • 2 Detection of Sulfhydration Using the Modified Biotin Switch Assay
        • 3 Detection of Sulfhydration Using the Maleimide Assay
        • 4 Summary
        • Acknowledgments
    • Section III: H2S in Cell Signaling in the Cardiovascular and Nervous System and Inflammatory Processes
      • Chapter Six: Intravital Microscopic Methods to Evaluate Anti-inflammatory Effects and Signaling Mechanisms Evoked by Hydrogen Sulfide
        • Abstract
        • 1 Introduction
        • 2 Molecular Determinants of Neutrophil/Endothelial Cell Adhesive Interactions
        • 3 Intravital Microscopic Approaches to Study Leukocyte/Endothelial Cell Adhesive Interactions
        • 4 Assessing Leukocyte Rolling, Adhesion, and Emigration in the Intact Microcirculation
        • 5 Detection of Chemokine and Adhesion Molecule Expression using Intravital Microscopy
        • 6 Intravital Microscopic Methods to Assess Changes in Microvascular Permeability
        • 7 Assessment of Reactive Oxygen Species Generation Using Intravital Microscopy
        • 8 Fluorescence Detection of Cell Injury using Intravital Microscopy
        • 9 Perfused Capillary Density Assessment with Intravital Microscopy
        • 10 Acute and Preconditioning-Induced Anti-inflammatory Actions of Hydrogen Sulfide: Assessment Using Intravital Microscopy
        • 11 Conclusion and Perspectives
        • Acknowledgment
      • Chapter Seven: Attenuation of Inflammatory Responses by Hydrogen Sulfide (H2S) in Ischemia/Reperfusion Injury
        • Abstract
        • 1 Introduction
        • 2 Ischemia–Reperfusion Injury
        • 3 Central Nervous System
        • 4 Respiratory System
        • 5 Cardiovascular System
        • 6 Gastrointestinal System
        • 7 Hepatobiliary System
        • 8 Renal System
        • 9 Musculoskeletal
        • 10 Summary
      • Chapter Eight: CD47-Dependent Regulation of H2S Biosynthesis and Signaling in T Cells
        • Abstract
        • 1 Introduction
        • 2 Regulation of H2S Biosynthesis in T Cells
        • 3 Catabolism of H2S
        • 4 Regulation of T cell Activation by H2S Signaling
        • 5 Autocrine and Paracrine Roles of H2S in T cell Activation
        • 6 Role of H2S in the Cytoskeleton
        • 7 T Cell Regulation by TSP1/CD47 Signaling
        • 8 H2S Regulation of Leukocyte Adhesion
        • 9 Role of H2S in Diseases Associated with Altered T cell Immunity
        • 10 Future Prospective
        • Acknowledgments
      • Chapter Nine: Anti-inflammatory and Cytoprotective Properties of Hydrogen Sulfide
        • Abstract
        • 1 Introduction
        • 2 Enzymatic Synthesis of H2S
        • 3 Healing and Resolution of Inflammation
        • 4 Mechanisms of Anti-inflammatory Effects of H2S
        • 5 Effects of H2S on Visceral Pain
        • 6 Cytoprotective Actions of H2S
        • 7 Therapeutic Applications of H2S-Releasing Drugs
        • Acknowledgments
      • Chapter Ten: H2S and Substance P in Inflammation
        • Abstract
        • 1 Introduction
        • 2 Disease Models Used to Study the Role of H2S and Substance P
        • 3 H2S and Substance P—What Are They Doing Together?
        • 4 Summary
        • Acknowledgments
      • Chapter Eleven: Role of Hydrogen Sulfide in Brain Synaptic Remodeling
        • Abstract
        • 1 Introduction
        • 2 Pharmacological and Physiological Effect of H2S
        • 3 Effect of H2S on the CNS
        • 4 Effect of H2S on Brain Cells (Astrocyte, Microglia, and Oligodendrocyte)
        • 5 Synapse
        • 6 Glia and Neurons Interactions
        • 7 Effect of H2S on Neuronal Redox Stress
        • 8 Effect of H2S on Glutamate Neurotransmission
        • 9 Effect of H2S on NMDA Receptor Regulation
        • 10 Effect of H2S on GABA-Mediated Neurotransmission
        • 11 Effect of H2S on Calmodulin Kinase
        • 12 Conclusion
        • Conflict of Interest
        • Acknowledgment
    • Section IV: H2S in Plants
      • Chapter Twelve: Detection of Thiol Modifications by Hydrogen Sulfide
        • Abstract
        • 1 Introduction
        • 2 Hydrogen Sulfide Acts as a Signal in Cells
        • 3 Modification of Thiols by Signaling Molecules
        • 4 Identification of Modified Thiols by Other Methods
        • 5 Experimental Protocols
        • 6 Caenorhabditis elegans as a Model Organism
        • 7 Growth of C. elegans
        • 8 Treatment of Samples with H2S
        • 9 Estimation of Toxicity of H2S Compounds
        • 10 Treatment of Samples with Thiol Tag
        • 11 Isolation and Analysis of Modified Proteins
        • 12 Estimation of Protein Concentrations in Samples
        • 13 Further Analysis and Identification of Modified Proteins
        • 14 Concluding Remarks
      • Chapter Thirteen: Analysis of Some Enzymes Activities of Hydrogen Sulfide Metabolism in Plants
        • Abstract
        • 1 Theory
        • 2 Equipment
        • 3 Materials
        • 4 Protocol 1
        • 5 Step 1: Analyze of l-/d-Cysteine Desulfhydrase Activity
        • 6 Protocol 2
        • 7 Step 1: Analyze of Sulfite Reductase Activity
        • 8 Protocol 3
        • 9 Step 1: Analyze of β-Cyano-l-Alanine Synthase Activity
        • 10 Protocol 4
        • 11 Step 1: Analyze of l-Cysteine Synthase Activity
        • Acknowledgment
      • Chapter Fourteen: Sulfide Detoxification in Plant Mitochondria
        • Abstract
        • 1 Introduction
        • 2 Methods
        • 3 Summary
        • Acknowledgments
    • Section V: Molecular Hydrogen
      • Chapter Fifteen: Molecular Hydrogen as a Novel Antioxidant: Overview of the Advantages of Hydrogen for Medical Applications
        • Abstract
        • 1 Introduction
        • 2 Comparison of H2 with Other Medical Gasses
        • 3 Oxidative Stress as Pathogenic Sources
        • 4 Physiological Roles of H2O2
        • 5 Measurement of H2 Gas Concentration
        • 6 Advantages of Hydrogen in Medical Applications
        • 7 Methods of Ingesting Molecular Hydrogen
        • 8 Medical Effects of H2
        • 9 Possible Molecular Mechanisms Underlying Various Effects of Molecular Hydrogen
        • 10 Unresolved Questions and Closing Remarks
    • Author Index
    • Subject Index

Product details

  • No. of pages: 370
  • Language: English
  • Copyright: © Academic Press 2015
  • Published: March 3, 2015
  • Imprint: Academic Press
  • eBook ISBN: 9780128016220
  • Hardcover ISBN: 9780128015117

About the Serial Volume Editors

Enrique Cadenas

ENRIQUE CADENAS, MD, PhD, received his PhD in biochemistry from the University of Buenos Aires, School of Medicine. He is professor of pharmacology and pharmaceutical sciences at the University of Southern California School of Pharmacy and of biochemistry and molecular biology at the University of Southern California Keck School of Medicine, and doctor honoris causa (medicine) at the University of Linköping, Sweden. Cadenas was president of the Society for Free Radical Research International (SFRRI) and is fellow of the Society for Free Radical Biology & Medicine. He served the scientific community by participating on NIH study sections (2002-2006; chair 2006-2008). His research interests include energy and redox metabolism in brain aging and the coordinated inflammatory-metabolic responses in brain and neurodegenerative diseases.

Affiliations and Expertise

Pharmacology & Pharmaceutical Sciences, School of Pharmacy, University of Southern California, USA

Lester Packer

Lester Packer
Lester Packer received a PhD in Microbiology and Biochemistry in 1956 from Yale University. In 1961, he joined the University of California at Berkeley serving as Professor of Cell and Molecular Biology until 2000, and then was appointed Adjunct Professor, Pharmacology and Pharmaceutical Sciences, School of Pharmacy at the University of Southern California.

Dr Packer received numerous distinctions including three honorary doctoral degrees, several distinguished Professor appointments. He was awarded Chevalier de l’Ordre National du Merite (Knight of the French National Order of Merit) and later promoted to the rank of Officier. He served as President of the Society for Free Radical Research International (SFRRI), founder and Honorary President of the Oxygen Club of California.

He has edited numerous books and published research; some of the most cited articles have become classics in the field of free radical biology:

Dr Packer is a member of many professional societies and editorial boards. His research elucidated - the Antioxidant Network concept. Exogenous lipoic acid was discovered to be one of the most potent natural antioxidants and placed as the ultimate reductant or in the pecking order of the “Antioxidant Network” regenerating vitamins C and E and stimulating glutathione synthesis, thereby improving the overall cellular antioxidant defense. The Antioxidant Network is a concept addressing the cell’s redox status. He established a world-wide network of research programs by supporting and co-organizing conferences on free radical research and redox biology in Asia, Europe, and America.

Affiliations and Expertise

Department of Molecular Pharmacology and Toxicology, School of Pharmaceutical Sciences, University of Southern California, USA

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