Physical Principles and Techniques of Protein Chemistry Part A - 1st Edition - ISBN: 9780124401013, 9780323141123

Physical Principles and Techniques of Protein Chemistry Part A

1st Edition

Editors: Sydney Leach
eBook ISBN: 9780323141123
Imprint: Academic Press
Published Date: 1st January 1969
Page Count: 544
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Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of maps from X-ray methods and the diffraction data from fibrous proteins. The subsequent chapters cover discussions on UV spectroscopy of proteins; luminescence properties of proteins and related compounds; and perturbation and flow methods for evaluation of proteins’ dynamic properties and rate constants. Other chapters deal with the evaluation of proteins’ dielectric properties using dielectric relaxation, electric birefringence, and dichroism techniques. The concluding chapters outline the theoretical and experimental advances of the electrophoretic and gel filtration methods for the study of protein structure and molecular weight. This book is of great value to chemists, biologists, and researchers who have great appreciation of protein chemistry.

Table of Contents

List of Contributors Preface Contents of Part B

  1. Electron Microscopy of Globular Proteins I. Introduction II. Electron Microscopy and the Electron Microscope III. The Enhancement of Contrast IV. The Preservation of Specimens V. Measurement VI. Examples of the Application of Electron Microscopy to the Study of Proteins VII. Prospects in the Electron Microscopy of Proteins References
  2. X-Ray Methods Glossary of Symbols I. Introduction II. The Nature of X-Ray Diffraction III. The Protein Crystal IV. The Reciprocal Lattice V. The Structure Factor VI. Fourier Synthesis VII. Methods of Structure Determination VIII. Fiber Diffraction IX. Further Reading References
  3. Ultraviolet Absorption Glossary of Symbols I. Introduction II. Basic Spectroscopic Considerations III. Chromophores of Proteins IV. Spectrophotometric Titrations V. Perturbation Difference Spectra VI. Applications of Difference Spectra VII. Determination of Tyrosine and Tryptophan VIII. Experimental Considerations IX. Prospects References
  4. Fluorescence of Proteins Glossary of Symbols I. Theory II. Technique III. Application to Proteins and Model Compounds References
  5. Perturbation and Flow Techniques Glossary of Symbols I. Introduction: On the Nature of Kinetic Analysis II. Perturbation Methods III. Flow Techniques IV. Combined Flow- and Temperature-Jump Techniques References
  6. Dielectric Properties of Proteins I. Dielectric Relaxation Glossary of Symbols I. Introduction II. Dielectrics in a Static Field III. Dielectrics in a Periodic Electric Field IV. Measurement of Dielectric Constant and Dielectric Loss V. Dielectric Relaxation of Proteins VI. Dielectric Relaxation


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© Academic Press 1969
Academic Press
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About the Editor

Sydney Leach

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