Physical Principles and Techniques of Protein Chemistry Part A

Physical Principles and Techniques of Protein Chemistry Part A

1st Edition - January 1, 1969

Write a review

  • Editor: Sydney Leach
  • eBook ISBN: 9780323141123

Purchase options

Purchase options
DRM-free (PDF)
Sales tax will be calculated at check-out

Institutional Subscription

Free Global Shipping
No minimum order


Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of maps from X-ray methods and the diffraction data from fibrous proteins. The subsequent chapters cover discussions on UV spectroscopy of proteins; luminescence properties of proteins and related compounds; and perturbation and flow methods for evaluation of proteins’ dynamic properties and rate constants. Other chapters deal with the evaluation of proteins’ dielectric properties using dielectric relaxation, electric birefringence, and dichroism techniques. The concluding chapters outline the theoretical and experimental advances of the electrophoretic and gel filtration methods for the study of protein structure and molecular weight. This book is of great value to chemists, biologists, and researchers who have great appreciation of protein chemistry.

Table of Contents

  • List of Contributors


    Contents of Part B

    1. Electron Microscopy of Globular Proteins

    I. Introduction

    II. Electron Microscopy and the Electron Microscope

    III. The Enhancement of Contrast

    IV. The Preservation of Specimens

    V. Measurement

    VI. Examples of the Application of Electron Microscopy to the Study of Proteins

    VII. Prospects in the Electron Microscopy of Proteins


    2. X-Ray Methods

    Glossary of Symbols

    I. Introduction

    II. The Nature of X-Ray Diffraction

    III. The Protein Crystal

    IV. The Reciprocal Lattice

    V. The Structure Factor

    VI. Fourier Synthesis

    VII. Methods of Structure Determination

    VIII. Fiber Diffraction

    IX. Further Reading


    3. Ultraviolet Absorption

    Glossary of Symbols

    I. Introduction

    II. Basic Spectroscopic Considerations

    III. Chromophores of Proteins

    IV. Spectrophotometric Titrations

    V. Perturbation Difference Spectra

    VI. Applications of Difference Spectra

    VII. Determination of Tyrosine and Tryptophan

    VIII. Experimental Considerations

    IX. Prospects


    4. Fluorescence of Proteins

    Glossary of Symbols

    I. Theory

    II. Technique

    III. Application to Proteins and Model Compounds


    5. Perturbation and Flow Techniques

    Glossary of Symbols

    I. Introduction: On the Nature of Kinetic Analysis

    II. Perturbation Methods

    III. Flow Techniques

    IV. Combined Flow- and Temperature-Jump Techniques


    6. Dielectric Properties of Proteins I. Dielectric Relaxation

    Glossary of Symbols

    I. Introduction

    II. Dielectrics in a Static Field

    III. Dielectrics in a Periodic Electric Field

    IV. Measurement of Dielectric Constant and Dielectric Loss

    V. Dielectric Relaxation of Proteins

    VI. Dielectric Relaxation of Polyamino Acids


    7. Dielectric Properties of Proteins II. Electric Birefringence and Dichroism

    Glossary of Symbols

    I. Introduction

    II. Outline of Theory

    III. Experimental Methods

    IV. Experimental Results

    V. Concluding Remarks


    8. Electrophoresis

    Glossary of Symbols

    I. Introduction

    II. Theory of Electromigration

    III. Moving-Boundary Electrophoresis

    IV. Zone Electrophoresis

    Appendix A. Differential Equation of Forced Diffusion in the Case of Electrophoresis

    Appendix B. Formulation of a Theory of Electrophoresis for Interactions between Macromolecules and Small Molecules


    9. Analytical Gel Filtration

    Glossary of Symbols

    I. Introduction

    II. Theory of Gel Filtration

    III. Experimental Aspects

    IV. Estimation of Molecular Weight

    V. Interpretation of Elution Profiles in Terms of Solute Composition

    VI. Studies of Rapid Chemical Equilibria

    VII. Concluding Remarks


    Author Index

    Subject Index

Product details

  • No. of pages: 544
  • Language: English
  • Copyright: © Academic Press 1969
  • Published: January 1, 1969
  • Imprint: Academic Press
  • eBook ISBN: 9780323141123

About the Editor

Sydney Leach

Ratings and Reviews

Write a review

There are currently no reviews for "Physical Principles and Techniques of Protein Chemistry Part A"