Physical Principles and Techniques of Protein Chemistry Part A

1st Edition - January 1, 1969
Editor: Sydney Leach
Language: English
eBook ISBN:
9 7 8 - 0 - 3 2 3 - 1 4 1 1 2 - 3

Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is… Read more

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Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of maps from X-ray methods and the diffraction data from fibrous proteins. The subsequent chapters cover discussions on UV spectroscopy of proteins; luminescence properties of proteins and related compounds; and perturbation and flow methods for evaluation of proteins’ dynamic properties and rate constants. Other chapters deal with the evaluation of proteins’ dielectric properties using dielectric relaxation, electric birefringence, and dichroism techniques. The concluding chapters outline the theoretical and experimental advances of the electrophoretic and gel filtration methods for the study of protein structure and molecular weight. This book is of great value to chemists, biologists, and researchers who have great appreciation of protein chemistry.

List of Contributors

Preface

Contents of Part B

1. Electron Microscopy of Globular Proteins

I. Introduction

II. Electron Microscopy and the Electron Microscope

III. The Enhancement of Contrast

IV. The Preservation of Specimens

V. Measurement

VI. Examples of the Application of Electron Microscopy to the Study of Proteins

VII. Prospects in the Electron Microscopy of Proteins

References

2. X-Ray Methods

Glossary of Symbols

I. Introduction

II. The Nature of X-Ray Diffraction

III. The Protein Crystal

IV. The Reciprocal Lattice

V. The Structure Factor

VI. Fourier Synthesis

VII. Methods of Structure Determination

VIII. Fiber Diffraction

IX. Further Reading

References

3. Ultraviolet Absorption

Glossary of Symbols

I. Introduction

II. Basic Spectroscopic Considerations

III. Chromophores of Proteins

IV. Spectrophotometric Titrations

V. Perturbation Difference Spectra

VI. Applications of Difference Spectra

VII. Determination of Tyrosine and Tryptophan

VIII. Experimental Considerations

IX. Prospects

References

4. Fluorescence of Proteins

Glossary of Symbols

I. Theory

II. Technique

III. Application to Proteins and Model Compounds

References

5. Perturbation and Flow Techniques

Glossary of Symbols

I. Introduction: On the Nature of Kinetic Analysis

II. Perturbation Methods

III. Flow Techniques

IV. Combined Flow- and Temperature-Jump Techniques

References

6. Dielectric Properties of Proteins I. Dielectric Relaxation

Glossary of Symbols

I. Introduction

II. Dielectrics in a Static Field

III. Dielectrics in a Periodic Electric Field

IV. Measurement of Dielectric Constant and Dielectric Loss

V. Dielectric Relaxation of Proteins

VI. Dielectric Relaxation of Polyamino Acids

References

7. Dielectric Properties of Proteins II. Electric Birefringence and Dichroism

Glossary of Symbols

I. Introduction

II. Outline of Theory

III. Experimental Methods

IV. Experimental Results

V. Concluding Remarks

References

8. Electrophoresis

Glossary of Symbols

I. Introduction

II. Theory of Electromigration

III. Moving-Boundary Electrophoresis

IV. Zone Electrophoresis

Appendix A. Differential Equation of Forced Diffusion in the Case of Electrophoresis

Appendix B. Formulation of a Theory of Electrophoresis for Interactions between Macromolecules and Small Molecules

References

9. Analytical Gel Filtration

Glossary of Symbols

I. Introduction

II. Theory of Gel Filtration

III. Experimental Aspects

IV. Estimation of Molecular Weight

V. Interpretation of Elution Profiles in Terms of Solute Composition

VI. Studies of Rapid Chemical Equilibria

VII. Concluding Remarks

References

Author Index

Subject Index