Physical Principles and Techniques of Protein Chemistry Part A - 1st Edition - ISBN: 9780124401013, 9780323141123

Physical Principles and Techniques of Protein Chemistry Part A

1st Edition

Editors: Sydney Leach
eBook ISBN: 9780323141123
Imprint: Academic Press
Published Date: 1st January 1969
Page Count: 544
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Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of maps from X-ray methods and the diffraction data from fibrous proteins. The subsequent chapters cover discussions on UV spectroscopy of proteins; luminescence properties of proteins and related compounds; and perturbation and flow methods for evaluation of proteins’ dynamic properties and rate constants. Other chapters deal with the evaluation of proteins’ dielectric properties using dielectric relaxation, electric birefringence, and dichroism techniques. The concluding chapters outline the theoretical and experimental advances of the electrophoretic and gel filtration methods for the study of protein structure and molecular weight. This book is of great value to chemists, biologists, and researchers who have great appreciation of protein chemistry.

Table of Contents

List of Contributors


Contents of Part B

1. Electron Microscopy of Globular Proteins

I. Introduction

II. Electron Microscopy and the Electron Microscope

III. The Enhancement of Contrast

IV. The Preservation of Specimens

V. Measurement

VI. Examples of the Application of Electron Microscopy to the Study of Proteins

VII. Prospects in the Electron Microscopy of Proteins


2. X-Ray Methods

Glossary of Symbols

I. Introduction

II. The Nature of X-Ray Diffraction

III. The Protein Crystal

IV. The Reciprocal Lattice

V. The Structure Factor

VI. Fourier Synthesis

VII. Methods of Structure Determination

VIII. Fiber Diffraction

IX. Further Reading


3. Ultraviolet Absorption

Glossary of Symbols

I. Introduction

II. Basic Spectroscopic Considerations

III. Chromophores of Proteins

IV. Spectrophotometric Titrations

V. Perturbation Difference Spectra

VI. Applications of Difference Spectra

VII. Determination of Tyrosine and Tryptophan

VIII. Experimental Considerations

IX. Prospects


4. Fluorescence of Proteins

Glossary of Symbols

I. Theory

II. Technique

III. Application to Proteins and Model Compounds


5. Perturbation and Flow Techniques

Glossary of Symbols

I. Introduction: On the Nature of Kinetic Analysis

II. Perturbation Methods

III. Flow Techniques

IV. Combined Flow- and Temperature-Jump Techniques


6. Dielectric Properties of Proteins I. Dielectric Relaxation

Glossary of Symbols

I. Introduction

II. Dielectrics in a Static Field

III. Dielectrics in a Periodic Electric Field

IV. Measurement of Dielectric Constant and Dielectric Loss

V. Dielectric Relaxation of Proteins

VI. Dielectric Relaxation of Polyamino Acids


7. Dielectric Properties of Proteins II. Electric Birefringence and Dichroism

Glossary of Symbols

I. Introduction

II. Outline of Theory

III. Experimental Methods

IV. Experimental Results

V. Concluding Remarks


8. Electrophoresis

Glossary of Symbols

I. Introduction

II. Theory of Electromigration

III. Moving-Boundary Electrophoresis

IV. Zone Electrophoresis

Appendix A. Differential Equation of Forced Diffusion in the Case of Electrophoresis

Appendix B. Formulation of a Theory of Electrophoresis for Interactions between Macromolecules and Small Molecules


9. Analytical Gel Filtration

Glossary of Symbols

I. Introduction

II. Theory of Gel Filtration

III. Experimental Aspects

IV. Estimation of Molecular Weight

V. Interpretation of Elution Profiles in Terms of Solute Composition

VI. Studies of Rapid Chemical Equilibria

VII. Concluding Remarks


Author Index

Subject Index


No. of pages:
© Academic Press 1969
Academic Press
eBook ISBN:

About the Editor

Sydney Leach

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