Microfluidic Cell Culture Systems - 1st Edition - ISBN: 9781437734591, 9781437734607

Microfluidic Cell Culture Systems

1st Edition

Editors: Christopher Bettinger Jeffrey T Borenstein Sarah L Tao
eBook ISBN: 9781437734607
Hardcover ISBN: 9781437734591
Imprint: William Andrew
Published Date: 14th December 2012
Page Count: 452
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Table of Contents


List of Contributors

Part 1: Materials and Fabrication Methods

Chapter 1. Microfluidic Cell Culture Platforms with Embedded Nanoscale Features

1.1 Introduction

1.2 Engineering of nanoscale features

1.3 Assembly of PDMS-based microfluidic platforms

1.4 Microfluidic platforms with embedded nanoscale features for cell studies

1.5 Summary



Chapter 2. Microvascular Networks for Tissue Engineering

2.1 Introduction

2.2 Characteristics of branched vascular networks

2.3 Fabrication of 2-D microvascular networks

2.4 Fabrication of 3-D microvascular networks

2.5 Microchannel topologies

2.6 Engineering meets biology: toward tissue engineering applications

2.7 Outlook and future challenges



Chapter 3. Microfluidics for Engineering 3D Tissues and Cellular Microenvironments

3.1 Introduction

3.2 Fabricating 3D tissue scaffolds using microfluidics

3.3 Dynamic 3D cell cultures within PDMS microfluidic devices

3.4 Hydrogel-based microfluidic culture devices and tissue scaffolds

3.5 Conclusion and future directions


Chapter 4. Fabrication of Advanced Microcontainer Arrays for Perfused 3D Cell Culture in Microfluidic Bioreactors

4.1 Introduction

4.2 Micromolding of cell container arrays

4.3 Introducing porosity

4.4 Functionalization of cell container arrays

4.5 Integration into microfluidic bioreactors

4.6 Conclusion


Chapter 5. Mechanobiological Approaches for the Control of Cell Motility

5.1 Introduction

5.2 Passive control of cell motility

5.3 Active control of cell motility

5.4 Summary


Chapter 6. Transport Models for Three-Dimensional Cell Culture Systems

6.1 Introduction

6.2 Fluid flow in cell culture systems

6.3 The theory of mass transport

6.4 Binding kinetics

6.5 Nondimensionalization

6.6 Order of magnitude analysis

6.7 Bulk parameter models

6.8 Examples

6.9 Microfluidic approaches for flow and transport control

6.10 Conclusion

Part 2: Tissue Engineering Strategies

Chapter 7. Microfluidic Systems for Controlling Stem Cells Microenvironments

7.1 Introduction

7.2 Microfluidic elements for cell culture

7.3 Controlling cellular microenvironments

7.4 Challenges and outlook



Chapter 8. Vascularization of Microfluidic Hydrogels

8.1 Introduction

8.2 Design criteria for microfluidic scaffolds

8.3 Forming and vascularizing microfluidic gels

8.4 Design considerations

8.5 Design algorithm

8.6 Summary



Chapter 9. Microfluidic Vascular Networks for Engineered Tissues

9.1 Introduction

9.2 3D Microfluidics fabrication techniques

9.3 Materials for microfluidic vasculature

9.4 Conclusion


Chapter 10. Microfluidic Approaches Toward Pulmonary Tissue Constructs

10.1 Introduction

10.2 Lung design

10.3 Engineering small airways

10.4 Engineering alveolar structures

10.5 Conclusions


Chapter 11. Microfabricated Kidney Tissue Models

11.1 Introduction

11.2 Significance of microfabricated kidney tissue models

11.3 Kidney structure and function relationship

11.4 Traditional kidney tissue models

11.5 Crucial signaling elements for kidney tissue models

11.6 Review of current microfabricated kidney tissue models

11.7 Summary and future direction


Chapter 12. Microfluidic Cell Culture Techniques

12.1 Fundamentals of microscale cell culture

12.2 Microfluidic cell culture systems

12.3 Microenvironmental stimuli

12.4 Microfluidic cell and tissue culture systems for drug discovery and studies in physiology

12.5 Conclusions

Part 3: In Vitro Models

Chapter 13. Functionalized Microfluidic Devices for Separation of Cell Phenotypes

13.1 Introduction

13.2 Negative selection for enrichment of target cells

13.3 Positive selection of target cells for diagnostic purposes

13.4 Capture and release of target cells from positive selection for tissue engineering purposes

13.5 Effect of shear on changes of receptor expression in cells

13.6 Conclusions


Chapter 14. Microfluidic Hepatotoxicity Platform

14.1 Introduction

14.2 Liver tissue microenvironment

14.3 Microfluidic liver design

14.4 Long-term hepatocyte culture

14.5 Summary



Chapter 15. Live Cell Analysis Under Shear Flow

15.1 Introduction

15.2 Flow control and well plate microfluidics

15.3 Cell biology applications

15.4 Microbiology applications

15.5 Summary


Chapter 16. Microfluidic Platforms for Evaluating Angiogenesis and Vasculogenesis

16.1 Introduction

16.2 Current methods in microfluidics

16.3 Conclusion and future directions


Chapter 17. Cardiovascular Disease/Discovery Models

17.1 Introduction

17.2 Cell culture in cellix’s Vena8 Endothelial+microfluidic biochips

17.3 Microfluidic cell culture biochip model for atherosclerosis

17.4 Conclusion




The fields of microfluidics and BioMEMS are significantly impacting cell biology research and applications through the application of engineering solutions to human disease and health problems. The dimensions of microfluidic channels are well suited to the physical scale of biological cells, and the many advantages of microfluidics make it an attractive platform for new techniques in biology.

This new professional reference applies the techniques of microsystems to cell culture applications. The authors provide a thoroughly practical guide to the principles of microfluidic device design and operation and their application to cell culture techniques. The resulting book is crammed with strategies and techniques that can be immediately deployed in the lab. Equally, the insights into cell culture applications will provide those involved in traditional microfluidics and BioMEMS with an understanding of the specific demands and opportunities presented by biological applications.

The goal is to guide new and interested researchers and technology developers to the important areas and state-of-the-practice strategies that will enhance the efficiency and value of their technologies, devices and biomedical products.

Key Features

  • Provides insights into the design and development of microfluidic systems with a specific focus on cell culture applications
  • Focuses on strategies and techniques for the design and fabrication of microfluidic systems and devices for cell culture
  • Provides balanced coverage of microsystems engineering and bioengineering


Academics, Researchers and Scientists working in a variety of fields, including (but not exclusive to) Biomedical Engineering, Materials Science, Microfabrication, Pharmaceuticals, Stem Cells and Regenerative Medicine technologies.


No. of pages:
© William Andrew 2013
William Andrew
eBook ISBN:
Hardcover ISBN:

About the Editors

Christopher Bettinger Editor

Department of Materials Science and Engineering, Carnegie Mellon University

Jeffrey T Borenstein Editor

Jeffrey T. Borenstein is Laboratory Technical Staff at the Charles Stark Draper Laboratory in Cambridge, Massachusetts, USA. Dr. Borenstein is a Technical Director for several of Draper’s programs in artificial organs, tissue engineering and implantable devices. His expertise is in MEMS fabrication technology, biological microsystems and the development of microdevices for therapeutic clinical applications. Dr. Borenstein currently serves as Principal Investigator for projects involving the application of microsystems technology towards engineered tissue constructs for organ assist devices and drug discovery, as well as implantable drug delivery systems for hearing loss and other diseases. These programs are funded by the Department of Defense, the National Institutes of Health and several commercial sponsors.

Affiliations and Expertise

Laboratory Technical Staff at the Charles Stark Draper Laboratory in Cambridge, Massachusetts, USA

Sarah L Tao Editor

Sarah Tao is Senior Manager, New Technologies at CooperVision, Inc. She was previously Senior Member Technical Staff, MEMS Design Group at Draper University, and Research Professor Equivalent, Bioengineering and Therapeutic Sciences at the University of California, San Francisco, USA. Her research interests lie in the areas of biomaterials, nanotechnology, regenerative medicine, drug delivery, BioMEMS, microfluidics and cell culture.

Affiliations and Expertise

Senior Manager, New Technologies at CooperVision, Inc., California, USA