Methods in Psychobiology

Methods in Psychobiology

Advanced Laboratory Techniques in Neuropsychology

1st Edition - January 1, 1977

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  • Editor: R. D. Myers
  • eBook ISBN: 9781483266978

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Methods in Psychobiology, Volume 3, Advanced Laboratory Techniques in Neuropsychology and Neurobiology is devoted in large measure to specialized techniques that are widely used in the fields of psychobiology and neurobiology. The experimental methods described form a companion to those presented in Volumes 1 and 2. Many of the procedures presented hinge directly on a mastery of the more rudimentary techniques dealt with in the earlier volumes. The emphasis in the content of this book is on one facet of neurobiology, the mammalian central nervous system. The central or peripheral processes that affect this system in a rat or larger laboratory animal constitute the principal focus of this series. This is due in large part to the enormous amount of research carried out with higher species and the generalizations that, it is hoped, may be drawn to the nervous system of the human. Topics discussed include neuronal connectivity; chemical lesioning of indoleamine pathways; evaluating sensation in animals after lesions, treatment with drugs, electrical stimulation, cooling, or radical changes in environment or development; and sleep in animals.

Table of Contents

  • List of Contributors


    Chapter 1 Anatomical Analysis of Neuronal Connectivity

    I. Introduction

    II. Anatomical Methods


    Chapter 2 Chemical Lesioning: Catecholamine Pathways

    I. Peripheral Administration of 6-Hydroxydopamine

    II. Central Administration of 6-Hydroxydopamine

    III. Preferential Reduction of Brain Norepinephrine

    IV. Preferential Destruction of Dopamine Pathways

    V. Animal Care after 6-Hydroxydopamine Administration

    VI. Evidence for Specificity of 6-Hydroxydopamine

    VII. Evidence for Activation of Compensatory Mechanisms Following 6-Hydroxydopamine Treatments

    VIII. Use of 6-Hydroxydopamine in Neurobiology and Neuropharmacology


    Chapter 3 Chemical Lesioning of Indoleamine Pathways

    I. Introduction

    II. Historical Review

    III. General Comments on the Drugs Studied and Their Administration

    IV. Effects of Intraventricular Administration of 5,6- or 5,7-Dihydroxytryptamine on CNS Monoamine Content in the Adult Rat

    V. Effects of Intracisternal Administration of 5,6- or 5,7-DHT on CNS Monoamine Content in the Adult Rat

    VI. Effects of Intraventricular Administration of 5,6- or 5,7-DHT or 6-OHDA on Monoamine Biosynthetic Enzymes

    VII. Effects of Intraventricular or Intracisternal 5,6- or 5,7-DHT on Brain and Spinal Cord 5-Hydroxyindoleacetic Acid

    VIII. Effects of Intraventricular Injections of 5,6- or 5,7-DHT on the Morphology of Central Monoamine Neurons

    IX. Effects of Intracerebral Injections of 5,6- and 5,7-DHT in the Adult Rat

    X. Affinity to Monoaminergic Uptake Sites as a Determinant of Selectivity of Action of Neurotoxic Tryptamines

    XI. Uptake Impairment as a Determinant of the Neurotoxic Potency of Tryptamine Analogs

    XII. Reduction of [3H] Amine Uptake as a Measure of Terminal Degeneration

    XIII. Distribution of [14C]5,6-DHT in Brain after Intraventricular Injection

    XIV. Effects of Drugs on the Toxic Actions of 5,6- or 5,7-DHT in the Adult Rat

    XV. Intracisternal, Intraventricular, or Peripheral Administration of 5,6- or 5,7-DHT in the Developing Rat

    XVI. Structure-Activity Relationships and Molecular Mechanism of Action of 5,6- and 5,7-DHT

    XVII. Nonspecific Actions of 5,6- and 5,7-DHT

    XVIII. Functional Consequences of Centrally Administered Dihydroxytryptamines

    XIX. Chemical Lesioning of Central Indoleamine Pathways by Substances Other Than 5,6- or 5,7-DHT

    XX. Conclusions


    Chapter 4 Determining Sensory Deficits in Animals

    I. Introduction

    II. The Neurological Examination

    III. Conditioning in the Examination of Sensory Deficits

    IV. Cortical-Evoked Potential Testing for Sensory Deficits

    V. Conclusion


    Chapter 5 Assessing the Effects of Early Experience

    I. Introduction

    II. The Developmental Continuum

    III. Generating Animals for Early Experience Research

    IV. Stimulation during the Prenatal Interval

    V. Stimulation during the Period of Infancy

    VI. Stimulation during the Postweaning Period

    VII. Statistical Analysis of Early Experience Experiments

    VIII. Uses of Early Experience Paradigms

    IX. Developmental Paradigms

    X. Other Factors

    XI. Conclusion


    Chapter 6 Measurements of Body Temperature

    I. Introduction: Some Milestones

    II. The Significance of Temperature Measurements in Various Body Loci

    III. Methods and Devices: General Considerations

    IV. Measurement and Recording of Body Temperatures

    V. Techniques of Measurement of Specific Temperatures in the Body

    VI. Concluding Remarks


    Chapter 7 Experimental Studies of Sleep in Animals

    I. Introduction

    II. Background

    III. Origin and Significance of Brain Waves

    IV. Techniques, Methods, and Instrumentation

    V. The Electroencephalogram: Synchronization and Desynchronization

    VI. Induction of Sleep

    VII. Sleep Deprivation Experiments

    VIII. Pharmacological Studies of Sleep

    IX. Sleep Data Processing Techniques


    Chapter 8 Induction and Measurement of Tremor and other Dyskinesias

    I. Introduction

    II. Methods of Cannulation

    III. Methods of Intracranial Injection

    IV. Evaluation of Motor Activity in Rats

    V. Evaluation of Motor Activity in Cats and Squirrel Monkeys

    VI. Verification of Cannula Placement



    Chapter 9 Hormone Administration: Peripheral and Intracranial Implants

    I. Introduction

    II. Systemic Administration

    III. Intracerebral Implantation of Crystalline Steroids


    Chapter 10 Chronic Methods: Intraventricular Infusion, Cerebrospinal Fluid Sampling, and Push-Pull Perfusion

    I. Introduction

    II. Method of Chronic Intraventricular Infusion

    III. Methods for Chronic Sampling of Cerebrospinal Fluid and Cerebral Tissue

    IV. Concluding Comment


    Chapter 11 Dietary Constituents and Self-Selection Procedures: Solid Foods

    I. Conceptual Background

    II. Preparation of Diets

    III. Some Useful Diets

    IV. Procedural Considerations in Self-Selection Experiments

    V. General Procedures for Conditioned Aversion and Preference Experiments

    VI. Interpretative Considerations

    VII. Future Research



    Subject Index

Product details

  • No. of pages: 352
  • Language: English
  • Copyright: © Academic Press 1977
  • Published: January 1, 1977
  • Imprint: Academic Press
  • eBook ISBN: 9781483266978

About the Editor

R. D. Myers

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