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Section 1. Introduction. Plant isozymes: a historical perspective (D.E. McMillin). New horizons in plant genetics (A. Kleinhofs). New challenges confronting plant breeders (G.A. Moore, G.B. Collins). 2. Isozymes in Basic Plant Genetics Research. Isozyme systems to study gene regulation during development: a lecture (M. Freeling). Allozymes in gene dosage studies (J.A. Birchler). Gene mapping (S.D. Tanksley). Plastid isozymes (N.F. Weeden). Genetics of mitochondrial isozymes (K.J. Newton). Evolution of plant isozymes (N.F. Weeden). Detection of somatic variation (M.W. Lassner, T.J. Orton). Measuring genetic variability in plant populations (A.H.D. Brown, B.S. Weir). Detection and measurement of natural selection (M.T. Clegg). Phylogenetic and systematic inferences from electrophoretic studies (D.J. Crawford). Estimation of mating systems (K. Ritland). 3. Isoenzymes in Plant Breeding. Identifying natural and parasexual hybrids (F. Lo Schiavo et al.). Pollen gene expression and selection: applications in plant breeding (D. Zamir). Introgression of genes from wild species (S.D. Tanksley). Introduction and characterization of alien genetic material (G.E. Hart, N.A. Tuleen). Applications of isozyme technology in breeding cross-pollinated crops (T.J. Orton). Electrophoretic variability and the pedigree breeding method (S. Mendlinger). Application of isozymes in tree breeding (W.T. Adams). The use of isozymes in plant disease research (J.J. Burdon, D.R. Marshall). 4. Proprietary Uses of Electrophoretic Variability. Genetic purity of commercial seed lots (P. Arus). Isozymic variation and plant breeders' right (D.C. Bailey). 5. Equipment and Procedures. An outline of general resource needs and procedures for the electrophoretic separation of active enzymes from plant tissues (C.R. Shields et al.). Enzyme activity staining (C.E. Vallejos).
Developments in Plant Genetics and Breeding, 1A: Isozymes in Plant Genetics and Breeding, Part A focuses on the advancements in the processes, methodologies, and approaches involved in the study of isozymes, including its role in plant genetics and breeding. The selection first elaborates on the historical perspectives of plant isozymes, plant genetics, and isozyme systems to study gene regulation during development. Discussions focus on the use of isozyme and similar comparisons to study differential gene regulation, gene preservation, dissemination of cultivars, propagation of cultivars and breeding lines, and studies on the effect of viral infection and hormones on isozyme expression. The text then examines allozymes in gene dosage studies, gene mapping, and plastid isozymes. The manuscript takes a look at the genetics of mitochondrial isozymes, evolution of plant isozymes, and detection of somatic variation. Topics include evolution of isozymes in plants, generation of isozymes, glutamate dehydrogenase, glutamate-oxaloacetate transaminase, and malate dehydrogenase. The text also ponders on enzyme activity staining, isozymic variation and plant breeders' rights, genetic purity of commercial seed lots, and use of isozymes in plant disease research. The selection is a valuable reference for researchers interested in the role of isozymes in plant genetics and breeding.
- © Elsevier Science 1983
- 1st August 1983
- Elsevier Science
- eBook ISBN:
Department of Horticulture, New Mexico State University, Las Cruces
Dr. Orton attended Michigan State University, where he earned a Ph.D. in Botany and Genetics. He was then an Assistant Professor and Geneticist at the University of California, Davis where he conducted research on the breeding and genetics of cool season vegetables. Subsequently, Dr. Orton was a Group Leader at Agrigenetics Corp. (Boulder, CO) where his focus was on the applications of biotechnology in plant breeding. Later, he was appointed as Senior Director at DNA Plant Technology Corp. (Cinnaminson, NJ) charged with all aspects of product development of fresh pre-cut/packaged vegetables, including plant breeding and applications of biotechnology. He developed new celery and carrot varieties, and was awarded a patent for food processing applications of vegetable varieties. He then joined the faculty of the School of Environmental and Biological Sciences at Rutgers University, where he served as Department Chair and Assistant Director of Rutgers Cooperative Extension before assuming his current position, Professor of Plant Biology in 2004. He is located at the Rutgers Agricultural Research and Extension Center in Bridgeton, NJ. His research and extension program focuses on fresh market and processing tomato genetics and breeding, specialty Capsicum peppers, seedless grapes, season extension in asparagus, and new product development in perishable commodities. Dr. Orton has taught undergraduate “Plant Breeding” at both UCDavis and at Rutgers, where he has co-taught for the past 15 years. He has been active in the development and dissemination of scholarship, publishing 48 refereed papers, 14 invited book chapters, 2 co-edited books (on applications of biotechnology in plant breeding), and a large complement of abstracts, non-refereed articles, and conference proceedings. He has been invited to present his research results at 50 scientific meetings and institutional seminars, and garnered $1.8 million to support his programs.
Agrigenetics Research Corporation
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