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Daniel L. Minor Jr. and Henry M. Colecraft
1. Correlating ion channel structure and function
2. TRPV3 Expression and Purification for Structure Determination by Cryo-EM
3. Methods to study phosphoinositide regulation of ion channels
4. Recombinant Expression and Purification of Pentameric Ligand-Gated Ion Channels for Cryo-EM Structural Studies
5. Detection of ligand binding to purified HCN channels using fluorescence-based size exclusion chromatography
Structural and chemical methods to assess channel dynamics
6. Structural Dynamics of Channels and Transporters by High-Speed Atomic Force Microscopy
7. Crosslinking for gating motions in GluRs
8. Single molecule FRET methodology for investigating glutamate receptors
9. Interrogating permeation and gating of Orai channels using chemical modification of cysteine residues
Channel reconstitution and studies in bilayers
10. A quantitative flux assay for the study of Cl- channels and transporters
11. Ion channels reconstitution in lipid bilayers
12. Combining in vitro translation with nanodisc technology and functional reconstitution of channels in planar lipid bilayers
Methods to assess oligomerization
13. The application of Poisson distribution statistics in ion channel reconstitution to determine oligomeric architecture
Ion Channels Part A, Volume 651 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of new developments on the topic. Each chapter is written by an international board of authors.
- Provides the authority and expertise of leading contributors from an international board of authors
- Presents the latest release in the Methods in Enzymology series
Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists
- No. of pages:
- © Academic Press 2021
- 1st June 2021
- Academic Press
- Hardcover ISBN:
Daniel L. Minor, Professor, Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA, USA
Professor, University of California San Francisco, CA, USA
Henry M. Colecraft, John C. Dalton Professor of Physiology and Cellular Biophysics & Cellular Biophysics, Columbia University College of Physicians & Surgeons, New York, NY, USA
Professor of Physiology and Cellular Biophysics and Cellular Biophysics, Columbia University College of Physicians and Surgeons, NY, USA
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