Ion Channels: Channel Biochemistry, Reconstitution, and Function
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Ion Channels: Channel Biochemistry, Reconstitution, and Function

1st Edition - May 29, 2021

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  • Editors: Daniel Minor, Henry Colecraft
  • eBook ISBN: 9780323853750
  • Hardcover ISBN: 9780323853743

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Description

Ion Channels Part A, Volume 651 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of new developments on the topic. Each chapter is written by an international board of authors.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

  • Preface

    Daniel L. Minor Jr. and Henry M. Colecraft

    Structure/function relationships

    1. Correlating ion channel structure and function

    Crina Nimigean

    2. TRPV3 Expression and Purification for Structure Determination by Cryo-EM

    Alexander Sobolevsky

    3. Methods to study phosphoinositide regulation of ion channels

    Tibor Rohacs

    4. Recombinant Expression and Purification of Pentameric Ligand-Gated Ion Channels for Cryo-EM Structural Studies

    Sudha Chakarpani

    5. Detection of ligand binding to purified HCN channels using fluorescence-based size exclusion chromatography

    Anna Moroni

    Structural and chemical methods to assess channel dynamics

    6. Structural Dynamics of Channels and Transporters by High-Speed Atomic Force Microscopy

    Simon Scheuring

    7. Crosslinking for gating motions in GluRs

    Andrew Plested

    8. Single molecule FRET methodology for investigating glutamate receptors

    Vasanthi Jayaraman

    9. Interrogating permeation and gating of Orai channels using chemical modification of cysteine residues

    Murali Prakriya

    Channel reconstitution and studies in bilayers

    10. A quantitative flux assay for the study of Cl- channels and transporters

    Alessio Accardi

    11. Ion channels reconstitution in lipid bilayers

    Eleonora Zakharian

    12. Combining in vitro translation with nanodisc technology and functional reconstitution of channels in planar lipid bilayers

    Gehard Thiel

    Methods to assess oligomerization

    13. The application of Poisson distribution statistics in ion channel reconstitution to determine oligomeric architecture

    Randy Stockbridge

Product details

  • No. of pages: 356
  • Language: English
  • Copyright: © Academic Press 2021
  • Published: May 29, 2021
  • Imprint: Academic Press
  • eBook ISBN: 9780323853750
  • Hardcover ISBN: 9780323853743

About the Serial Volume Editors

Daniel Minor

Daniel L. Minor, Professor, Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA, USA

Affiliations and Expertise

Professor, University of California San Francisco, CA, USA

Henry Colecraft

Henry M. Colecraft, John C. Dalton Professor of Physiology and Cellular Biophysics & Cellular Biophysics, Columbia University College of Physicians & Surgeons, New York, NY, USA

Affiliations and Expertise

Professor of Physiology and Cellular Biophysics and Cellular Biophysics, Columbia University College of Physicians and Surgeons, NY, USA

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