G Protein-Coupled Receptors Part A

G Protein-Coupled Receptors Part A

2nd Edition - September 29, 2017

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  • Editor: Arun K. Shukla
  • eBook ISBN: 9780128133200
  • Hardcover ISBN: 9780128133194

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G Protein Coupled Receptors, Second Edition, Volume 143, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. It contains a wide array of topics about the G protein coupled receptors, as well as updates of chapters from the first edition.

Key Features

  • Contains contributions from experts in the field from across the world
  • Covers a wide array of topics on G protein coupled receptors
  • Includes analysis based topics


Researchers and students in cell, molecular and developmental biology

Table of Contents

  • 1. Generating recombinant peptide ligands for GPCRs and their single step purification
    2. Ca2+ release assay to study downstream pathways of GPCR signal transduction
    3. Expression and purification of GPCR interacting protein, arrestin
    4. Isolation and characterization of chemosensory GPCRs from exosomes
    5. Characterization of chemosensory bitter taste receptor signalling
    6. Reporter gene assays for investigating GPCR signaling
    7. Measuring binding affinity of regulatory motifs (peptides) to purified G proteins
    8. Measuring nucleotide exchange on purified G proteins
    9. Qualitative and quantitative measurement of GPCR-G protein activity in living cells
    10. Computational approaches to study GPCR activation
    11. Visualization and quantification of GPCR trafficking in mammalian cells by confocal microscopy
    12. Computational studies of βarrestin activation
    13. Measuring G protein coupling using Glosensor assay
    14. Computational investigations into GPCR dimerization
    15. cAMP Assays in GPCR Drug Discovery
    16. Assays of adrenal GPCR signaling and regulation: Measuring adrenal beta-arrestin activity in vivo through plasma membrane recruitment

Product details

  • No. of pages: 260
  • Language: English
  • Copyright: © Academic Press 2017
  • Published: September 29, 2017
  • Imprint: Academic Press
  • eBook ISBN: 9780128133200
  • Hardcover ISBN: 9780128133194

About the Serial Volume Editor

Arun K. Shukla

Arun K. Shukla
Dr. Arun K. Shukla obtained his M.Sc. (Master in Science) from the Center for Biotechnology at the Jawaharlal Nehru University in New Delhi, India. Dr. Shukla did his Ph.D. from the Department of Molecular Membrane Biology at the Max Planck Institute of Biophysics in Frankfurt, Germany. His Ph.D. research work was focused on structural studies of G Protein-Coupled Receptors (GPCRs).

Dr. Shukla subsequently carried out his post-doctoral work in the Department of Medicine at the Duke University in North Carolina, USA. During his post-doctoral research work, Dr. Shukla focused on understanding the biophysical and structural basis of ß-arrestin mediated regulation of GPCRs and non-canonical GPCR signaling. Dr. Shukla has served as an Assistant Professor in the Department of Medicine at the Duke University in Durham, North Carolina, USA.

Dr. Shukla is currently an Assistant Professor in Department of Biological Sciences and Bioengineering at the Indian Institute of Technology, Kanpur, India. Dr. Shukla is also an Intermediate Fellow of the Wellcome Trust-DBT India Alliance. The research program in Dr. Shukla’s laboratory is focused on understanding the molecular mechanism of activation, signaling and regulation of G Protein-Coupled Receptors.

Affiliations and Expertise

Assistant Professor, Indian Institute of Technology, Kanpur, India

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