Comparative Plant Virology - 2nd Edition - ISBN: 9780123741547, 9780080920962

Comparative Plant Virology

2nd Edition

Authors: Roger Hull
eBook ISBN: 9780080920962
Hardcover ISBN: 9780123741547
Imprint: Academic Press
Published Date: 23rd January 2009
Page Count: 400
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Comparative Plant Virology provides a complete overview of our current knowledge of plant viruses, including background information on plant viruses and up-to-date aspects of virus biology and control. It deals mainly with concepts rather than detail. The focus will be on plant viruses but due to the changing environment of how virology is taught, comparisons will be drawn with viruses of other kingdomes, animals, fungi and bacteria. It has been written for students of plant virology, plant pathology, virology and microbiology who have no previous knowledge of plant viruses or of virology in general.

Key Features

  • Boxes highlight important information such as virus definition and taxonomy
  • Includes profiles of 32 plant viruses that feature extensively in the text
  • Full color throughout


Advanced undergraduate and graduate students in basic and applied plant virology, plant pathology, microbiology, genetics and molecular biology, biological control, ecology, evolution, and related aspects of plant science

Table of Contents

Section I: Introduction to Plant Viruses

Chapter 1. What is a virus? I Introduction II History III Definition of a virus A How viruses differ from other plant pathogens B Are viruses alive? IV Classification and nomenclature of viruses A Virus classification B Families, genera and species C Naming of virus species D. Acronyms and abbreviations E Plant virus classification F Virus strains G Use of virus names V Viruses of other kingdoms VI Summary

Chapter 2. Overview of plant viruses I. Introduction II Economic losses due to plant viruses III Virus profiles IV Macroscopic symptoms A. Local symptoms B. Systemic symptoms

1. Effects on plant size 2. Mosaic patterns and related symptoms 3. Yellows diseases 4. Leaf rolling 5 Ring spot diseases 6 Necrotic diseases 7. Developmental abnormalities 8 Wilting 9. Recovery from disease 10. Genetic effects C. The cryptoviruses D. Diseases caused by viral complexes E. Agents inducing virus-like symptoms

V Histological changes A. Necrosis B. Hypoplasia C. Hyperplasia

1. Cells are larger than normal 2. Cell division in differentiated cells 3. Abnormal division in cambial cells

VI Cytopathological effects A. Effects on cell structures

1. Nuclei 2. Mitochondria 3. Chloroplasts 4. Cell walls 5. Cell death B. Virus induced structures in the cytoplasm 1. Accumulations of virus particles 2. Aggregates of virus-encoded proteins 3. Caulimovirus inclusions C. Why inclusion bodies? D. Cytoplasmic structures resembling those induced by viruses.

VII The host range of viruses A. Limitations in host range studies B. Patterns of host range C. The determinants of host range

1. Initial events 2. Expression and replication 3. Cell-to-cell movement 4. Stimulation of host-cell defences

VIII Viruses of other kingdoms IX Summary

Chapter 3. Agents that resemble or alter plant virus diseases I Viroids A Classification of viroids B. Pathology of viroids

1. Macroscopic disease symptoms 2. Cytopathic effects 3. Location of viroids in plants 4. Movement in the plant 5. Transmission 6. Epidemiology C Properties of viroid RNAs 1. Sequence and structure 2. Replication 3. Recombination between viroids D. Molecular basis for biological activity E. Diagnostic procedures for viroids

II Phytoplasma III Satellite viruses and satellite RNAs A Satellite plant viruses (A-type) B. Satellite RNAs (satRNAs)

1. Large satellite RNAs (B-type) 2. Small linear satellite RNAs (C-type)
  1. Small circular satellite RNAs (D-type)
  2. Satellite-like RNAs a. A satellite RNA of Groundnut rosette virus (GRV). b Ancillary RNAs of Beet necrotic yellow vein virus (BNYVV)
  3. Molecular basis for symptom modulation C. Satellite DNAs D. Discussion IV Defective and defective-interfering nucleic acids
    1. Group 1: Single deletion D-RNAs
    2. Group 2: Multiple deletion D- and DI-RNAs
    3. Defective DNAs associated with DNA viruses V. Other kingdoms VI Summary

Chapter 4. Plant virus origins and evolution I. Introduction II Virus evolution
A Origins of viruses B Virus variation C Types of evolution

1. Microevolution and macroevolution 2. Sequence divergence or convergence 3. Modular evolution 4. Sources of viral genes a. Replicases i. RNA replicases ii. Reverse transcriptase iii DNA replicases b. Proteases c. Coat proteins d. Cell-to-cell movement proteins e. Suppressors of gene silencing D Selection pressures for evolution 1. Adaptation to niches 2. Maximizing the variation 3. Controlling the variation a. Muller’s ratchet b. Does Muller’s ratchet operate with plant viruses? 4. Role of selection pressure 5. Selection pressure by host plants E. Timeline for evolution 1. Non-constant rates of evolution 2. Estimated rates of evolution

III Evidence for virus evolution A. Geminiviruses B. Closteroviruses C. Luteoviruses IV Co-evolution of viruses with their hosts and vectors V Other kingdoms VI Summary

Section II: What is a Virus Made of?

Chapter 5. Architecture and assembly of virus particles I. Introduction II. Methods A Chemical and biochemical studies B. Methods for studying size and fine structure of viruses

1. Hydrodynamic measurements 2. Electron microscopy 3. X-ray crystallography 4. Neutron small-angle scattering 5. Atomic force microscopy 6. Mass spectrometry 7. Serological methods 8. Stabilizing bonds

III Architecture of rod-shaped viruses A. Introduction B. Structure of TMV

  1. General features
  2. Virus structure C. Assembly of TMV
  3. Properties of the coat protein
  4. Assembly of TMV coat protein
  5. Assembly of the TMV rod a. Assembly in vitro b. Assembly in vivo IV Architecture of isometric viruses A. Introduction B. Possible icosahedra C. Clustering of subunits D. Quasiequivalence V Small icosahedral viruses A. Subunit structure B Virion structure
    1. T = 1 particles
    2. Other particles based on T = 1 symmetry a. Bacilliform particles based on T = 1 symmetry b. Geminiviruses
    3. T = 3 particles a. Bacilliform particles based on T = 3 symmetry b. Pseudo T = 3 symmetry
    4. T = 7 particles C The arrangement of nucleic acid within icosahedral viruses
    5. RNA structure
    6. Interactions between RNA and protein in small isometric viruses D. Stabilization of small isometric particles
    7. Protein-RNA stabilization
    8. Protein-protein stabilization
    9. Protein-protein + protein-RNA stabilization VI. More complex isometric viruses VII Enveloped viruses VIII Assembly of icosahedral viruses A Bromoviruses B. RNA selection during assembly of plant reoviruses IX General considerations X Viruses of other kingdoms XI Summary

Chapter 6. Plant viral genomes I. Introduction II. General properties of plant viral genomes A. Information content B Economy of use of genomic nucleic acids C. The functions of viral gene products

1. Functional proteins a. Proteins initiating infection b. Proteins that replicate the viral genome c. Proteins that process viral gene products d. Proteins that facilitate viral movement through the host e. Overcoming host defence systems f. Proteins that facilitate host to host movement D. Nucleic acids 1. Multipartite genomes 2. Nucleic acid structures 3. Non-coding regions a. Eng-group structures b. 5’ and 3’ non-coding regions c. Intergenic regions

III. Plant viral genome organization A. The structure of the genome B. Recognizing activities of viral genes

1. Location of spontaneous or artificially-induced mutations 2. Recombinant viruses 3. Expression of the gene in a transgenic plant 4. Hybrid arrest and hybrid select procedures 5. Sequence comparison with genes of known function 6. Functional regions within a gene

IV Other kingdoms V. Summary

Chapter 7. Expression of viral genomes I Stages in virus infection cycle II Virus entry and uncoating A. Virus entry B. Uncoating

  1. Uncoating of TMV
  2. Uncoating of Brome mosaic virus and Southern bean mosaic virus
  3. Uncoating of Turnip yellow mosaic virus
  4. Uncoating other plant viruses III Initial translation of the viral genome IV Synthesis of mRNAs A. Negative-sense single-stranded RNA viruses B. Double-stranded RNA viruses C. DNA viruses
    1. Caulimoviridae
    2. Geminiviridae V Plant viral genome strategies A. The eukaryotic translation system constraint B. Virus strategies to overcome Eukaryotic translation constraints
    3. Strategy 1: Polyproteins
    4. Strategy 2: Sub-genomic RNAs
    5. Strategy 3: Multipartite genomes
    6. Strategy 4. Splicing
    7. Strategy 5: Translation of both viral and complementary strands (ambisense)
    8. Strategy 6: Internal initiation
    9. Strategy 7: Leaky scanning a. Two initiation sites on one ORF (two start) b. Overlapping ORFs c. Two or more consecutive ORFs
    10. Strategy 8: Non-AUG start codons
    11. Strategy 9: Transactivation
    12. Strategy 10: Translational (ribosome) shunt
    13. Strategy 11: Read-through proteins
    14. Strategy 12: Frameshift proteins C. Control of translation
    15. Cap but no Poly(A) tail
    16. Poly(A) tail but no cap
    17. Neither cap or Poly (A) tail
    18. Cap snatching
    19. 5’ UTR D. Discussion VI Other kingdoms VII Summary

Chapter 8 Virus replication I. Host functions used by plant viruses II. Methods for studying viral replication III. Replication of plus-sense single-stranded RNA viruses A. Viral templates B. Replicase

  1. RNA-dependent RNA polymerase
  2. Helicases
  3. Methyl transferase activity
  4. Organization of functional domains in viral ORFs C. Sites of replication D. Mechanism of replication E. Discussion IV. Replication of negative-sense single-stranded RNA viruses V. Replication of double-stranded RNA viruses VI. Replication of reverse transcribing viruses A. Introduction B. Reverse transcriptase C. Replication of “caulimoviruses”
  5. Replication pathway
  6. Inclusion bodies VII. Replication of single-stranded DNA viruses A. Geminivirus replication B. Geminivirus Rep proteins VIII Faults in replication A. Mutation B. Recombination
    1. DNA virus recombination
    2. RNA virus recombination
    3. Recombination and integrated viral sequences IX Other kingdoms X Summary

Section III: How do Plant Viruses Work?

Chapter 9 Virus-host interactions: 1. Plant level I Movement and final distribution A. Intracellular movement B. Intercellular movement

1. Plasmodesmata 2. Movement proteins (MPs) 3. What actually moves 4. Cell-to-cell movement of viroids 5. Complementation 6. Rate of cell-to-cell movement C Systemic movement 1. Steps in systemic movement 2. Form in which virus is transported 3. Rate of systemic movement 4. Movement in the xylem D. Final distribution in the plant E. Outstanding questions on plant virus movement

II Effects on plant metabolism A. Nucleic acids and proteins B. Lipids C. Carbohydrates D. Photosynthesis E. Respiration F. Transpiration G. Low molecular weight compounds III Processes involved in symptom production A. Sequestration of raw materials B. Effects on growth C. Effects on chloroplasts D. Mosaic symptoms E. Role of membranes IV Other kingdoms V Summary

Chapter 10. Virus-plant interactions: 2. Molecular level I. Introduction II. Host response to inoculation A. Immunity B. Subliminal infection C. Non-permissive infection

  1. Local infection a. Host protein changes in hypersensitive response b. Local acquired resistance
  2. Systemic infection
  3. Systemic acquired resistance
  4. Programmed cell death D. Permissive infection
  5. Systemic host response
  6. Virus genes involved III Interactions between viruses A. Interactions between related viruses B. Interactions between unrelated viruses
  7. Complete dependence for disease
  8. Incomplete dependence for disease
  9. Synergistic effects on viral replication
  10. Effects on virus movement C. Interactions between viruses and other plant pathogens IV Other kingdoms V. Summary

Chapter 11. Virus-plant interactions – 2. RNA silencing I. Introduction II. Mechanism of silencing A. The basic pathway B. Components of the system

  1. dsRNA
  2. Dicer
  3. Products of Dicer
  4. RISC C. Results of the system III. Systemic silencing IV. Overcoming silencing A. Suppression of silencing
  5. Protein suppressors of silencing
  6. Nucleic acid suppressors of silencing B. Avoidance of silencing V. Silencing and symptoms A. Recovery B. Dark-green islands and mosaics C. miRNA D. siRNA effects E. Synergistic effects F. Other activities of silencing suppressors VI. Transcriptional and translational silencing VII. Evolutionary aspects VIII. RNA silencing in animal and other viruses IX. Summary

Section IV: Plant Viruses in Agriculture and Industry

Chapter 12. Plant to plant movement I. Introduction II. Transmission via plant material A. Mechanical transmission B. Seed transmission C. Pollen transmission D. Vegetative transmission E. Grafting III. Transmission by invertebrates A. Relationship between plant viruses and insects B. Non-persistent transmission by insects

  1. Features of non-persistent transmission
  2. Virus-vector relationships a. Direct capsid interaction b. Indirect interaction involving helper components C. Persistent transmission by insects
  3. Circulative viruses a. Features of circulative virus-vector interaction b. Dependent transmission
  4. Propagative viruses
  5. Thrip transmission of Tospoviruses D. Transmission by beetles E. Nematode transmission of viruses
  6. Features of nematode transmission
  7. Virus-nematode relationships IV. Fungal transmission of viruses V. Other Kingdoms VI. Summary

Chapter 13. Plant viruses in the field: Diagnosis, epidemiology and ecology I. Diagnosis A. Introduction B. Methods involving biology of the virus

1. Indicator hosts 2. Host range 3. Methods of transmission 4. Cytological effects 5. Mixed infections. C. Methods depending on physical properties of the virus particle 1. Physical properties 2. Electron microscopy D. Methods depending on properties of viral proteins 1. Serology 2. Types of antisera 3. Methods for detecting antibody-virus combination a. ELISA procedures b. Serologically specific electron microscopy c. Electrophoretic procedures d. Dot blots E. Methods involving properties of the viral nucleic acid 1. Type and size of nucleic acid 2. Cleavage patterns of DNA 3. Hybridization procedures 4. Dot blots 5. Polymerase chain reaction 6. DNA microarray F. Decision making on diagnosis

II Epidemiology and ecology A. Epidemiology of viruses in agriculture

  1. Primary infections
  2. Secondary spread B. Plant viruses in the natural environment C. Emergence of new viruses III Other kingdoms IV Summary

Chapter 14. Conventional control I Introduction II Avoiding infection A. Removal of sources of infection B. Virus-free seed C. Virus-free vegetative stocks D Modified agronomic practices E. Quarantine regulations III. Stopping the vector A. Airborne vectors

1. Insecticides 2. Insect deterrents 3. Agronomic techniques B. Soilborne vectors 1. Nematodes 2. Fungi

IV. Protecting the plant A. Protection by a plant pathogen B. Antiviral chemicals. V. Conventional resistance to plant viruses A. Introduction B. Genetics of resistance to viruses C. Tolerance D. Use of conventional resistance for control

1. Immunity 2. Field resistance 3. Tolerance

VI. Strategies for control VII. Other Kingdoms VII. Summary

Chapter 15. Transgenic plants and viruses I. Transgenic protection against plant viruses A. Introduction B. Natural resistance genes II Pathogen-derived resistance A. Protein-based protection

  1. Transgenic plants expressing viral coat protein
  2. Other viral proteins B. Nucleic acid –based protection
  3. RNA-mediated protection
  4. Molecular basis of RNA-mediated protection
  5. Sequences for RNA-mediated protection
  6. Ribozymes
  7. Relationship between natural cross protection and protection in transgenic plants.
  8. Transgenic protection by satellite and DI nucleic acid C. Other forms of transgenic protection D. Field release of transgenic plants
  9. Potential risks
  10. Field performance III. Possible uses of plant viruses in gene technology A. DNA viruses as gene vectors
  11. Caulimoviruses
  12. Geminiviruses B. RNA viruses as gene vectors C. Viruses as sources of control elements for transgenic plants
  13. DNA promoters
  14. RNA promoters
  15. Translation enhancers D. Viruses for producing vaccines
  16. Vaccines using plant virus vectors
  17. Viruses for presenting heterologous peptides a. Cowpea mosaic virus b. Tobacco mosaic virus E. Viruses in plant functional genomics F. Plant viruses in nanotechnology IV. Other kingdoms V. Summary

Appendix – Profiles

Subject Index


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About the Author

Roger Hull

Roger Hull graduated in Botany from Cambridge University in 1960, and subsequently studied plant virus epidemiology at London University’s Wye College, gaining a PhD in 1964. He lectured on agricultural botany there between 1960 and 1965.

He was seconded to Makerere University in Kampala, Uganda in 1964 where he taught, and learnt tropical agricultural botany and studied the epidemiology of groundnut rosette disease. By watching aphids land on groundnut plants he gained an understanding of the edge effect of spread of virus into the field. In 1965 Roger Hull joined Roy Markham at the ARC Virus Research Unit in Cambridge, UK where he worked on biophysical and biochemical characterization of a range of viruses, especially Alfalfa mosaic virus. This work continued when he moved to the John Innes Institute, Norwich with Roy Markham in 1968. There Dr Hull became a project leader and deputy head of the Virus Research Department. In 1974 he spent a sabbatical year with Bob Shepherd in the University of California, Davis where he worked on the characterization of cauliflower mosaic virus. There he was introduced to the early stages of molecular biology which changed the direction of his research. On returning to the John Innes Institute he applied a molecular biological approach to the study of cauliflower mosaic virus elucidating that it replicated by reverse transcription, the first plant virus being shown to do so. Involvement with the Rockefeller Rice Biotechnology Program reawakened his interest in tropical agricultural problems and he led a large group studying the viruses of the rice tungro disease complex. He also promoted the use of transgenic technology to the control of virus diseases and was in the forefront in discussing biosafety issues associated with this approach. Moving from rice to bananas (plantains) his group was among those who discovered that the genome of banana streak badnavirus was integrated into the host genome and in certain cultivars was activated to give episomal infection – another first for plant viruses. He retired at the statutory age in 1997.

Dr Hull is an Honorary Professor at Peking and Fudan Universities, a Doctoris Honoris Causa at the University of Perpignan, France, and a Fellow of the American Phytopathological Society. He is an Emeritus Fellow at the John Innes Centre where he continued research on banana streak virus for five or more years after retirement. He has published over 225 peer-reviewed papers on plant virology, many reviews and four books including the previous edition of Plant Virology and Comparative Plant Virology.

In retirement Roger Hull became involved in promoting the uptake of transgenic technology by developing countries as one approach to alleviating food insecurity. He is on the International faculty of e-learning diploma course training decision makers, mainly in developing countries, in plant biotechnology regulation. His other interests are gardening, bird watching, travelling and his children and grandchildren.

Affiliations and Expertise

John Innes Center, Norwich, UK