Comparative Plant Virology

Comparative Plant Virology

2nd Edition - January 23, 2009

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  • Author: Roger Hull
  • eBook ISBN: 9780080920962
  • Hardcover ISBN: 9780123741547

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Comparative Plant Virology provides a complete overview of our current knowledge of plant viruses, including background information on plant viruses and up-to-date aspects of virus biology and control. It deals mainly with concepts rather than detail. The focus will be on plant viruses but due to the changing environment of how virology is taught, comparisons will be drawn with viruses of other kingdomes, animals, fungi and bacteria. It has been written for students of plant virology, plant pathology, virology and microbiology who have no previous knowledge of plant viruses or of virology in general.

Key Features

  • Boxes highlight important information such as virus definition and taxonomy
  • Includes profiles of 32 plant viruses that feature extensively in the text
  • Full color throughout


Advanced undergraduate and graduate students in basic and applied plant virology, plant pathology, microbiology, genetics and molecular biology, biological control, ecology, evolution, and related aspects of plant science

Table of Contents

  • Section I: Introduction to Plant Viruses

    Chapter 1. What is a virus?
    I Introduction
    II History
    III Definition of a virus
    A How viruses differ from other plant pathogens
    B Are viruses alive?
    IV Classification and nomenclature of viruses
    A Virus classification
    B Families, genera and species
    C Naming of virus species
    D. Acronyms and abbreviations
    E Plant virus classification
    F Virus strains
    G Use of virus names
    V Viruses of other kingdoms
    VI Summary

    Chapter 2. Overview of plant viruses
    I. Introduction
    II Economic losses due to plant viruses
    III Virus profiles
    IV Macroscopic symptoms
    A. Local symptoms
    B. Systemic symptoms
    1. Effects on plant size
    2. Mosaic patterns and related symptoms
    3. Yellows diseases
    4. Leaf rolling
    5 Ring spot diseases
    6 Necrotic diseases
    7. Developmental abnormalities
    8 Wilting
    9. Recovery from disease
    10. Genetic effects
    C. The cryptoviruses
    D. Diseases caused by viral complexes
    E. Agents inducing virus-like symptoms
    V Histological changes
    A. Necrosis
    B. Hypoplasia
    C. Hyperplasia
    1. Cells are larger than normal
    2. Cell division in differentiated cells
    3. Abnormal division in cambial cells
    VI Cytopathological effects
    A. Effects on cell structures
    1. Nuclei
    2. Mitochondria
    3. Chloroplasts
    4. Cell walls
    5. Cell death
    B. Virus induced structures in the cytoplasm
    1. Accumulations of virus particles
    2. Aggregates of virus-encoded proteins
    3. Caulimovirus inclusions
    C. Why inclusion bodies?
    D. Cytoplasmic structures resembling those induced by viruses.
    VII The host range of viruses
    A. Limitations in host range studies
    B. Patterns of host range
    C. The determinants of host range
    1. Initial events
    2. Expression and replication
    3. Cell-to-cell movement
    4. Stimulation of host-cell defences
    VIII Viruses of other kingdoms
    IX Summary

    Chapter 3. Agents that resemble or alter plant virus diseases
    I Viroids
    A Classification of viroids
    B. Pathology of viroids
    1. Macroscopic disease symptoms
    2. Cytopathic effects
    3. Location of viroids in plants
    4. Movement in the plant
    5. Transmission
    6. Epidemiology
    C Properties of viroid RNAs
    1. Sequence and structure
    2. Replication
    3. Recombination between viroids
    D. Molecular basis for biological activity
    E. Diagnostic procedures for viroids
    II Phytoplasma
    III Satellite viruses and satellite RNAs
    A Satellite plant viruses (A-type)
    B. Satellite RNAs (satRNAs)
    1. Large satellite RNAs (B-type)
    2. Small linear satellite RNAs (C-type)
    3. Small circular satellite RNAs (D-type)
    4. Satellite-like RNAs
    a. A satellite RNA of Groundnut rosette virus (GRV).
    b Ancillary RNAs of Beet necrotic yellow vein virus (BNYVV)
    5. Molecular basis for symptom modulation
    C. Satellite DNAs
    D. Discussion
    IV Defective and defective-interfering nucleic acids
    1. Group 1: Single deletion D-RNAs
    2. Group 2: Multiple deletion D- and DI-RNAs
    3. Defective DNAs associated with DNA viruses
    V. Other kingdoms
    VI Summary

    Chapter 4. Plant virus origins and evolution
    I. Introduction
    II Virus evolution
    A Origins of viruses
    B Virus variation
    C Types of evolution
    1. Microevolution and macroevolution
    2. Sequence divergence or convergence
    3. Modular evolution
    4. Sources of viral genes
    a. Replicases
    i. RNA replicases
    ii. Reverse transcriptase
    iii DNA replicases
    b. Proteases
    c. Coat proteins
    d. Cell-to-cell movement proteins
    e. Suppressors of gene silencing
    D Selection pressures for evolution
    1. Adaptation to niches
    2. Maximizing the variation
    3. Controlling the variation
    a. Muller’s ratchet
    b. Does Muller’s ratchet operate with plant viruses?
    4. Role of selection pressure
    5. Selection pressure by host plants
    E. Timeline for evolution
    1. Non-constant rates of evolution
    2. Estimated rates of evolution
    III Evidence for virus evolution
    A. Geminiviruses
    B. Closteroviruses
    C. Luteoviruses
    IV Co-evolution of viruses with their hosts and vectors
    V Other kingdoms
    VI Summary

    Section II: What is a Virus Made of?

    Chapter 5. Architecture and assembly of virus particles
    I. Introduction
    II. Methods
    A Chemical and biochemical studies
    B. Methods for studying size and fine structure of viruses
    1. Hydrodynamic measurements
    2. Electron microscopy
    3. X-ray crystallography
    4. Neutron small-angle scattering
    5. Atomic force microscopy
    6. Mass spectrometry
    7. Serological methods
    8. Stabilizing bonds
    III Architecture of rod-shaped viruses
    A. Introduction
    B. Structure of TMV
    1. General features
    2. Virus structure
    C. Assembly of TMV
    1. Properties of the coat protein
    2. Assembly of TMV coat protein
    3. Assembly of the TMV rod
    a. Assembly in vitro
    b. Assembly in vivo
    IV Architecture of isometric viruses
    A. Introduction
    B. Possible icosahedra
    C. Clustering of subunits
    D. Quasiequivalence
    V Small icosahedral viruses
    A. Subunit structure
    B Virion structure
    1. T = 1 particles
    2. Other particles based on T = 1 symmetry
    a. Bacilliform particles based on T = 1 symmetry
    b. Geminiviruses
    3. T = 3 particles
    a. Bacilliform particles based on T = 3 symmetry
    b. Pseudo T = 3 symmetry
    4. T = 7 particles
    C The arrangement of nucleic acid within icosahedral viruses
    1. RNA structure
    2. Interactions between RNA and protein in small isometric viruses
    D. Stabilization of small isometric particles
    1. Protein-RNA stabilization
    2. Protein-protein stabilization
    3. Protein-protein + protein-RNA stabilization
    VI. More complex isometric viruses
    VII Enveloped viruses
    VIII Assembly of icosahedral viruses
    A Bromoviruses
    B. RNA selection during assembly of plant reoviruses
    IX General considerations
    X Viruses of other kingdoms
    XI Summary

    Chapter 6. Plant viral genomes
    I. Introduction
    II. General properties of plant viral genomes
    A. Information content
    B Economy of use of genomic nucleic acids
    C. The functions of viral gene products
    1. Functional proteins
    a. Proteins initiating infection
    b. Proteins that replicate the viral genome
    c. Proteins that process viral gene products
    d. Proteins that facilitate viral movement through the host
    e. Overcoming host defence systems
    f. Proteins that facilitate host to host movement
    D. Nucleic acids
    1. Multipartite genomes
    2. Nucleic acid structures
    3. Non-coding regions
    a. Eng-group structures
    b. 5’ and 3’ non-coding regions
    c. Intergenic regions
    III. Plant viral genome organization
    A. The structure of the genome
    B. Recognizing activities of viral genes
    1. Location of spontaneous or artificially-induced mutations
    2. Recombinant viruses
    3. Expression of the gene in a transgenic plant
    4. Hybrid arrest and hybrid select procedures
    5. Sequence comparison with genes of known function
    6. Functional regions within a gene
    IV Other kingdoms
    V. Summary

    Chapter 7. Expression of viral genomes
    I Stages in virus infection cycle
    II Virus entry and uncoating
    A. Virus entry
    B. Uncoating
    1. Uncoating of TMV
    2. Uncoating of Brome mosaic virus and Southern bean mosaic virus
    3. Uncoating of Turnip yellow mosaic virus
    4. Uncoating other plant viruses
    III Initial translation of the viral genome
    IV Synthesis of mRNAs
    A. Negative-sense single-stranded RNA viruses
    B. Double-stranded RNA viruses
    C. DNA viruses
    1. Caulimoviridae
    2. Geminiviridae
    V Plant viral genome strategies
    A. The eukaryotic translation system constraint
    B. Virus strategies to overcome Eukaryotic translation constraints
    1. Strategy 1: Polyproteins
    2. Strategy 2: Sub-genomic RNAs
    3. Strategy 3: Multipartite genomes
    4. Strategy 4. Splicing
    5. Strategy 5: Translation of both viral and complementary strands (ambisense)
    6. Strategy 6: Internal initiation
    7. Strategy 7: Leaky scanning
    a. Two initiation sites on one ORF (two start)
    b. Overlapping ORFs
    c. Two or more consecutive ORFs
    8. Strategy 8: Non-AUG start codons
    9. Strategy 9: Transactivation
    10. Strategy 10: Translational (ribosome) shunt
    11. Strategy 11: Read-through proteins
    12. Strategy 12: Frameshift proteins
    C. Control of translation
    1. Cap but no Poly(A) tail
    2. Poly(A) tail but no cap
    3. Neither cap or Poly (A) tail
    4. Cap snatching
    5. 5’ UTR
    D. Discussion
    VI Other kingdoms
    VII Summary

    Chapter 8 Virus replication
    I. Host functions used by plant viruses
    II. Methods for studying viral replication
    III. Replication of plus-sense single-stranded RNA viruses
    A. Viral templates
    B. Replicase
    1. RNA-dependent RNA polymerase
    2. Helicases
    3. Methyl transferase activity
    4. Organization of functional domains in viral ORFs
    C. Sites of replication
    D. Mechanism of replication
    E. Discussion
    IV. Replication of negative-sense single-stranded RNA viruses
    V. Replication of double-stranded RNA viruses
    VI. Replication of reverse transcribing viruses
    A. Introduction
    B. Reverse transcriptase
    C. Replication of “caulimoviruses”
    1. Replication pathway
    2. Inclusion bodies
    VII. Replication of single-stranded DNA viruses
    A. Geminivirus replication
    B. Geminivirus Rep proteins
    VIII Faults in replication
    A. Mutation
    B. Recombination
    1. DNA virus recombination
    2. RNA virus recombination
    3. Recombination and integrated viral sequences
    IX Other kingdoms
    X Summary

    Section III: How do Plant Viruses Work?

    Chapter 9 Virus-host interactions: 1. Plant level
    I Movement and final distribution
    A. Intracellular movement
    B. Intercellular movement
    1. Plasmodesmata
    2. Movement proteins (MPs)
    3. What actually moves
    4. Cell-to-cell movement of viroids
    5. Complementation
    6. Rate of cell-to-cell movement
    C Systemic movement
    1. Steps in systemic movement
    2. Form in which virus is transported
    3. Rate of systemic movement
    4. Movement in the xylem
    D. Final distribution in the plant
    E. Outstanding questions on plant virus movement
    II Effects on plant metabolism
    A. Nucleic acids and proteins
    B. Lipids
    C. Carbohydrates
    D. Photosynthesis
    E. Respiration
    F. Transpiration
    G. Low molecular weight compounds
    III Processes involved in symptom production
    A. Sequestration of raw materials
    B. Effects on growth
    C. Effects on chloroplasts
    D. Mosaic symptoms
    E. Role of membranes
    IV Other kingdoms
    V Summary

    Chapter 10. Virus-plant interactions: 2. Molecular level
    I. Introduction
    II. Host response to inoculation
    A. Immunity
    B. Subliminal infection
    C. Non-permissive infection
    1. Local infection
    a. Host protein changes in hypersensitive response
    b. Local acquired resistance
    2. Systemic infection
    3. Systemic acquired resistance
    4. Programmed cell death
    D. Permissive infection
    1. Systemic host response
    2. Virus genes involved
    III Interactions between viruses
    A. Interactions between related viruses
    B. Interactions between unrelated viruses
    1. Complete dependence for disease
    2. Incomplete dependence for disease
    3. Synergistic effects on viral replication
    4. Effects on virus movement
    C. Interactions between viruses and other plant pathogens
    IV Other kingdoms
    V. Summary

    Chapter 11. Virus-plant interactions – 2. RNA silencing
    I. Introduction
    II. Mechanism of silencing
    A. The basic pathway
    B. Components of the system
    1. dsRNA
    2. Dicer
    3. Products of Dicer
    4. RISC
    C. Results of the system
    III. Systemic silencing
    IV. Overcoming silencing
    A. Suppression of silencing
    1. Protein suppressors of silencing
    2. Nucleic acid suppressors of silencing
    B. Avoidance of silencing
    V. Silencing and symptoms
    A. Recovery
    B. Dark-green islands and mosaics
    C. miRNA
    D. siRNA effects
    E. Synergistic effects
    F. Other activities of silencing suppressors
    VI. Transcriptional and translational silencing
    VII. Evolutionary aspects
    VIII. RNA silencing in animal and other viruses
    IX. Summary

    Section IV: Plant Viruses in Agriculture and Industry

    Chapter 12. Plant to plant movement
    I. Introduction
    II. Transmission via plant material
    A. Mechanical transmission
    B. Seed transmission
    C. Pollen transmission
    D. Vegetative transmission
    E. Grafting
    III. Transmission by invertebrates
    A. Relationship between plant viruses and insects
    B. Non-persistent transmission by insects
    1. Features of non-persistent transmission
    2. Virus-vector relationships
    a. Direct capsid interaction
    b. Indirect interaction involving helper components
    C. Persistent transmission by insects
    1. Circulative viruses
    a. Features of circulative virus-vector interaction
    b. Dependent transmission
    2. Propagative viruses
    3. Thrip transmission of Tospoviruses
    D. Transmission by beetles
    E. Nematode transmission of viruses
    1. Features of nematode transmission
    2. Virus-nematode relationships
    IV. Fungal transmission of viruses
    V. Other Kingdoms
    VI. Summary

    Chapter 13. Plant viruses in the field: Diagnosis, epidemiology and ecology
    I. Diagnosis
    A. Introduction
    B. Methods involving biology of the virus
    1. Indicator hosts
    2. Host range
    3. Methods of transmission
    4. Cytological effects
    5. Mixed infections.
    C. Methods depending on physical properties of the virus particle
    1. Physical properties
    2. Electron microscopy
    D. Methods depending on properties of viral proteins
    1. Serology
    2. Types of antisera
    3. Methods for detecting antibody-virus combination
    a. ELISA procedures
    b. Serologically specific electron microscopy
    c. Electrophoretic procedures
    d. Dot blots
    E. Methods involving properties of the viral nucleic acid
    1. Type and size of nucleic acid
    2. Cleavage patterns of DNA
    3. Hybridization procedures
    4. Dot blots
    5. Polymerase chain reaction
    6. DNA microarray
    F. Decision making on diagnosis
    II Epidemiology and ecology
    A. Epidemiology of viruses in agriculture
    1. Primary infections
    2. Secondary spread
    B. Plant viruses in the natural environment
    C. Emergence of new viruses
    III Other kingdoms
    IV Summary

    Chapter 14. Conventional control
    I Introduction
    II Avoiding infection
    A. Removal of sources of infection
    B. Virus-free seed
    C. Virus-free vegetative stocks
    D Modified agronomic practices
    E. Quarantine regulations
    III. Stopping the vector
    A. Airborne vectors
    1. Insecticides
    2. Insect deterrents
    3. Agronomic techniques
    B. Soilborne vectors
    1. Nematodes
    2. Fungi
    IV. Protecting the plant
    A. Protection by a plant pathogen
    B. Antiviral chemicals.
    V. Conventional resistance to plant viruses
    A. Introduction
    B. Genetics of resistance to viruses
    C. Tolerance
    D. Use of conventional resistance for control
    1. Immunity
    2. Field resistance
    3. Tolerance
    VI. Strategies for control
    VII. Other Kingdoms
    VII. Summary

    Chapter 15. Transgenic plants and viruses
    I. Transgenic protection against plant viruses
    A. Introduction
    B. Natural resistance genes
    II Pathogen-derived resistance
    A. Protein-based protection
    1. Transgenic plants expressing viral coat protein
    2. Other viral proteins
    B. Nucleic acid –based protection
    1. RNA-mediated protection
    2. Molecular basis of RNA-mediated protection
    3. Sequences for RNA-mediated protection
    4. Ribozymes
    5. Relationship between natural cross protection and protection in transgenic plants.
    6. Transgenic protection by satellite and DI nucleic acid
    C. Other forms of transgenic protection
    D. Field release of transgenic plants
    1. Potential risks
    2. Field performance
    III. Possible uses of plant viruses in gene technology
    A. DNA viruses as gene vectors
    1. Caulimoviruses
    2. Geminiviruses
    B. RNA viruses as gene vectors
    C. Viruses as sources of control elements for transgenic plants
    1. DNA promoters
    2. RNA promoters
    3. Translation enhancers
    D. Viruses for producing vaccines
    1. Vaccines using plant virus vectors
    2. Viruses for presenting heterologous peptides
    a. Cowpea mosaic virus
    b. Tobacco mosaic virus
    E. Viruses in plant functional genomics
    F. Plant viruses in nanotechnology
    IV. Other kingdoms
    V. Summary

    Appendix – Profiles

    Subject Index

Product details

  • No. of pages: 400
  • Language: English
  • Copyright: © Academic Press 2009
  • Published: January 23, 2009
  • Imprint: Academic Press
  • eBook ISBN: 9780080920962
  • Hardcover ISBN: 9780123741547

About the Author

Roger Hull

Roger Hull
Roger Hull graduated in botany from Cambridge University and undertook his graduate studies in plant virus diagnostics and epidemiology at London University. He lectured on agricultural botany there and at Makerere University in Uganda. In 1965 he moved to fundamental studies of plant viruses, first at Cambridge in the United Kingdom and then at the John Innes Institute (now Centre) in Norwich. He spent a sabbatical year (1974) at University of California, Davis, where he learnt the fundamentals of the newly developing molecular biology technology. He applied to this to plant virus characterisation, diagnostics and virus control, especially in tropical crops such as rice and plantain bananas. He retired in 1997 but continued research, lecturing and book writing. Dr Hull was an honorary professor at University of East Anglia in the UK and Peking and Fudan Universities in China, a Doctoris Honoris Causa at the University of Perpignan in France, and a Fellow of the American Phytopathological Society. He has published over 250 peer-reviewed papers on plant virology and more than 40 reviews in scientific journals, and has authored five books. In retirement Roger Hull also became involved in promoting the uptake of transgenic technology by developing countries as one approach to alleviating food insecurity. He was on the International faculty of the e-learning diploma course training decision makers, mainly in developing countries, in plant biotechnology regulation.

Affiliations and Expertise

Retired from John Innes Centre, Norwich, United Kingdom

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