Bioprocess Engineering

An Introductory Engineering and Life Science Approach

1st Edition - October 31, 2013

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Author: Kim Gail Clarke
eBook ISBN: 9781782421689

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Description

Biotechnology is an expansive field incorporating expertise in both the life science and engineering disciplines. In biotechnology, the scientist is concerned with developing the most favourable biocatalysts, while the engineer is directed towards process performance, defining conditions and strategies that will maximize the production potential of the biocatalyst. Increasingly, the synergistic effect of the contributions of engineering and life sciences is recognised as key to the translation of new bioproducts from the laboratory bench to commercial bioprocess. Fundamental to the successful realization of the bioprocess is a need for process engineers and life scientists competent in evaluating biological systems from a cross-disciplinary viewpoint. Bioprocess engineering aims to generate core competencies through an understanding of the complementary biotechnology disciplines and their interdependence, and an appreciation of the challenges associated with the application of engineering principles in a life science context. Initial chapters focus on the microbiology, biochemistry and molecular biology that underpin biocatalyst potential for product accumulation. The following chapters develop kinetic and mass transfer principles that quantify optimum process performance and scale up. The text is wide in scope, relating to bioprocesses using bacterial, fungal and enzymic biocatalysts, batch, fed-batch and continuous strategies and free and immobilised configurations.

Key Features

Details the application of chemical engineering principles for the development, design, operation and scale up of bioprocesses
Details the knowledge in microbiology, biochemistry and molecular biology relevant to bioprocess design, operation and scale up
Discusses the significance of these life sciences in defining optimum bioprocess performance

Readership

Professionals in biotechnology disciplines; Postgraduate and upper-level undergraduate students in bioprocess engineering and life sciences; Researchers in biotechnology R&D and operations; Project managers in commercial bioprocess environments

Dedication

List of figures

List of plates

Preface

About the author

Chapter 1: Historical development: from ethanol to biopharmeceuticals

Abstract:

Chapter 2: Microbiology

Abstract:

2.1 Microorganisms: the core of cellular bioprocesses

2.2 Cellular structure and sites of metabolic reactions

2.3 Classification according to carbon and energy requirements

2.4 Nutrient requirements

Chapter 3: Metabolic macromolecules

Abstract:

3.1 Carbohydrates

3.2 Lipids

3.3 Proteins

3.4 Nucleosides, nucleotides and nucleic acids

Chapter 4: Molecular biology

Abstract:

4.1 Replication, transcription and translation

4.2 Genetic regulation

4.3 Genetic modification

Chapter 5: Carbon metabolism

Abstract:

5.1 Energy generation, storage and transfer

5.2 Catabolic pathways: energy generation

5.3 Anabolic pathways: energy utilisation

Chapter 6: Enzymes as biocatalysts

Abstract:

6.1 Enzyme kinetics with no inhibition

6.2 Enzyme kinetics with inhibition

6.3 Enzyme reactors with soluble enzymes

6.4 Enzyme reactors with immobilised enzymes

Chapter 7: Microbial kinetics during batch, continuous and fed-batch processes

Abstract:

7.1 The nutrient medium

7.2 Batch process design equations

7.3 Continuous process design equations

7.4 Fed-batch bioprocess design equations

Chapter 8: The oxygen transfer rate and overall volumetric oxygen transfer coefficient

Abstract:

8.1 Oxygen transfer design equations

8.2 Measurement of the oxygen transfer rate

Chapter 9: Bioprocess scale up

Abstract:

9.1 Scale up with constant oxygen transfer rate

9.2 Scale up with constant mixing

9.3 Scale up with constant shear stress

9.4 Scale up with constant flow regime

Chapter 10: Bioprocess asepsis and sterility

Abstract:

10.1 Heat sterilisation of media and equipment

10.2 Filter sterilisation of air

Chapter 11: Downstream processing

Abstract:

11.1 Overview of potential recovery operations

11.2 Separation of cells and extracellular fluid

11.3 Cell rupture and separation of cell extract

11.4 Concentration and purification of soluble products

Index

Product details

No. of pages: 266
Language: English
Published: October 31, 2013
Imprint: Woodhead Publishing
eBook ISBN: 9781782421689

About the Author

Kim Gail Clarke

Prof Kim Gail Clarke is an academic in the Department of Process Engineering, University of Stellenbosch, and a registered Professional Engineer. She holds a PhD and a BSc (Chemical Engineering), both from the University of Cape Town, SA, and an MSc (Biological and Chemical Engineering) from the University of Birmingham, UK. She has lectured university courses in bioprocess engineering for over 15 years and has twice received the University of Stellenbosch Rector’s Award for Excellence in Education (2007 and 2010). She heads a research group in bioprocess engineering which focuses on the application of chemical engineering principles in the development and optimization of biological processes. Her research is specifically directed towards fundamental kinetic and mass transfer studies which quantify the principles governing process performance and define process strategies for optimization of the production potential of the biocatalyst.

Affiliations and Expertise

University of Stellenbosch, South Africa

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