Bioprocess Engineering

Bioprocess Engineering

An Introductory Engineering and Life Science Approach

1st Edition - October 31, 2013

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  • Author: Kim Gail Clarke
  • eBook ISBN: 9781782421689

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Biotechnology is an expansive field incorporating expertise in both the life science and engineering disciplines. In biotechnology, the scientist is concerned with developing the most favourable biocatalysts, while the engineer is directed towards process performance, defining conditions and strategies that will maximize the production potential of the biocatalyst. Increasingly, the synergistic effect of the contributions of engineering and life sciences is recognised as key to the translation of new bioproducts from the laboratory bench to commercial bioprocess. Fundamental to the successful realization of the bioprocess is a need for process engineers and life scientists competent in evaluating biological systems from a cross-disciplinary viewpoint. Bioprocess engineering aims to generate core competencies through an understanding of the complementary biotechnology disciplines and their interdependence, and an appreciation of the challenges associated with the application of engineering principles in a life science context. Initial chapters focus on the microbiology, biochemistry and molecular biology that underpin biocatalyst potential for product accumulation. The following chapters develop kinetic and mass transfer principles that quantify optimum process performance and scale up. The text is wide in scope, relating to bioprocesses using bacterial, fungal and enzymic biocatalysts, batch, fed-batch and continuous strategies and free and immobilised configurations.

Key Features

  • Details the application of chemical engineering principles for the development, design, operation and scale up of bioprocesses
  • Details the knowledge in microbiology, biochemistry and molecular biology relevant to bioprocess design, operation and scale up
  • Discusses the significance of these life sciences in defining optimum bioprocess performance


Professionals in biotechnology disciplines; Postgraduate and upper-level undergraduate students in bioprocess engineering and life sciences; Researchers in biotechnology R&D and operations; Project managers in commercial bioprocess environments

Table of Contents

  • Dedication

    List of figures

    List of plates


    About the author

    Chapter 1: Historical development: from ethanol to biopharmeceuticals


    Chapter 2: Microbiology


    2.1 Microorganisms: the core of cellular bioprocesses

    2.2 Cellular structure and sites of metabolic reactions

    2.3 Classification according to carbon and energy requirements

    2.4 Nutrient requirements

    Chapter 3: Metabolic macromolecules


    3.1 Carbohydrates

    3.2 Lipids

    3.3 Proteins

    3.4 Nucleosides, nucleotides and nucleic acids

    Chapter 4: Molecular biology


    4.1 Replication, transcription and translation

    4.2 Genetic regulation

    4.3 Genetic modification

    Chapter 5: Carbon metabolism


    5.1 Energy generation, storage and transfer

    5.2 Catabolic pathways: energy generation

    5.3 Anabolic pathways: energy utilisation

    Chapter 6: Enzymes as biocatalysts


    6.1 Enzyme kinetics with no inhibition

    6.2 Enzyme kinetics with inhibition

    6.3 Enzyme reactors with soluble enzymes

    6.4 Enzyme reactors with immobilised enzymes

    Chapter 7: Microbial kinetics during batch, continuous and fed-batch processes


    7.1 The nutrient medium

    7.2 Batch process design equations

    7.3 Continuous process design equations

    7.4 Fed-batch bioprocess design equations

    Chapter 8: The oxygen transfer rate and overall volumetric oxygen transfer coefficient


    8.1 Oxygen transfer design equations

    8.2 Measurement of the oxygen transfer rate

    Chapter 9: Bioprocess scale up


    9.1 Scale up with constant oxygen transfer rate

    9.2 Scale up with constant mixing

    9.3 Scale up with constant shear stress

    9.4 Scale up with constant flow regime

    Chapter 10: Bioprocess asepsis and sterility


    10.1 Heat sterilisation of media and equipment

    10.2 Filter sterilisation of air

    Chapter 11: Downstream processing


    11.1 Overview of potential recovery operations

    11.2 Separation of cells and extracellular fluid

    11.3 Cell rupture and separation of cell extract

    11.4 Concentration and purification of soluble products


Product details

  • No. of pages: 266
  • Language: English
  • Copyright: © Woodhead Publishing 2013
  • Published: October 31, 2013
  • Imprint: Woodhead Publishing
  • eBook ISBN: 9781782421689

About the Author

Kim Gail Clarke

Prof Kim Gail Clarke is an academic in the Department of Process Engineering, University of Stellenbosch, and a registered Professional Engineer. She holds a PhD and a BSc (Chemical Engineering), both from the University of Cape Town, SA, and an MSc (Biological and Chemical Engineering) from the University of Birmingham, UK. She has lectured university courses in bioprocess engineering for over 15 years and has twice received the University of Stellenbosch Rector’s Award for Excellence in Education (2007 and 2010). She heads a research group in bioprocess engineering which focuses on the application of chemical engineering principles in the development and optimization of biological processes. Her research is specifically directed towards fundamental kinetic and mass transfer studies which quantify the principles governing process performance and define process strategies for optimization of the production potential of the biocatalyst.

Affiliations and Expertise

University of Stellenbosch, South Africa

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