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Bioprocess Engineering - 1st Edition - ISBN: 9781782421672, 9781782421689

Bioprocess Engineering

1st Edition

An Introductory Engineering and Life Science Approach

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Author: Kim Gail Clarke
Hardcover ISBN: 9781782421672
eBook ISBN: 9781782421689
Imprint: Woodhead Publishing
Published Date: 31st October 2013
Page Count: 266
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Biotechnology is an expansive field incorporating expertise in both the life science and engineering disciplines. In biotechnology, the scientist is concerned with developing the most favourable biocatalysts, while the engineer is directed towards process performance, defining conditions and strategies that will maximize the production potential of the biocatalyst. Increasingly, the synergistic effect of the contributions of engineering and life sciences is recognised as key to the translation of new bioproducts from the laboratory bench to commercial bioprocess. Fundamental to the successful realization of the bioprocess is a need for process engineers and life scientists competent in evaluating biological systems from a cross-disciplinary viewpoint. Bioprocess engineering aims to generate core competencies through an understanding of the complementary biotechnology disciplines and their interdependence, and an appreciation of the challenges associated with the application of engineering principles in a life science context. Initial chapters focus on the microbiology, biochemistry and molecular biology that underpin biocatalyst potential for product accumulation. The following chapters develop kinetic and mass transfer principles that quantify optimum process performance and scale up. The text is wide in scope, relating to bioprocesses using bacterial, fungal and enzymic biocatalysts, batch, fed-batch and continuous strategies and free and immobilised configurations.

Key Features

  • Details the application of chemical engineering principles for the development, design, operation and scale up of bioprocesses
  • Details the knowledge in microbiology, biochemistry and molecular biology relevant to bioprocess design, operation and scale up
  • Discusses the significance of these life sciences in defining optimum bioprocess performance


Professionals in biotechnology disciplines; Postgraduate and upper-level undergraduate students in bioprocess engineering and life sciences; Researchers in biotechnology R&D and operations; Project managers in commercial bioprocess environments

Table of Contents


List of figures

List of plates


About the author

Chapter 1: Historical development: from ethanol to biopharmeceuticals


Chapter 2: Microbiology


2.1 Microorganisms: the core of cellular bioprocesses

2.2 Cellular structure and sites of metabolic reactions

2.3 Classification according to carbon and energy requirements

2.4 Nutrient requirements

Chapter 3: Metabolic macromolecules


3.1 Carbohydrates

3.2 Lipids

3.3 Proteins

3.4 Nucleosides, nucleotides and nucleic acids

Chapter 4: Molecular biology


4.1 Replication, transcription and translation

4.2 Genetic regulation

4.3 Genetic modification

Chapter 5: Carbon metabolism


5.1 Energy generation, storage and transfer

5.2 Catabolic pathways: energy generation

5.3 Anabolic pathways: energy utilisation

Chapter 6: Enzymes as biocatalysts


6.1 Enzyme kinetics with no inhibition

6.2 Enzyme kinetics with inhibition

6.3 Enzyme reactors with soluble enzymes

6.4 Enzyme reactors with immobilised enzymes

Chapter 7: Microbial kinetics during batch, continuous and fed-batch processes


7.1 The nutrient medium

7.2 Batch process design equations

7.3 Continuous process design equations

7.4 Fed-batch bioprocess design equations

Chapter 8: The oxygen transfer rate and overall volumetric oxygen transfer coefficient


8.1 Oxygen transfer design equations

8.2 Measurement of the oxygen transfer rate

Chapter 9: Bioprocess scale up


9.1 Scale up with constant oxygen transfer rate

9.2 Scale up with constant mixing

9.3 Scale up with constant shear stress

9.4 Scale up with constant flow regime

Chapter 10: Bioprocess asepsis and sterility


10.1 Heat sterilisation of media and equipment

10.2 Filter sterilisation of air

Chapter 11: Downstream processing


11.1 Overview of potential recovery operations

11.2 Separation of cells and extracellular fluid

11.3 Cell rupture and separation of cell extract

11.4 Concentration and purification of soluble products



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© Woodhead Publishing 2013
31st October 2013
Woodhead Publishing
Hardcover ISBN:
eBook ISBN:

About the Author

Kim Gail Clarke

Prof Kim Gail Clarke is an academic in the Department of Process Engineering, University of Stellenbosch, and a registered Professional Engineer. She holds a PhD and a BSc (Chemical Engineering), both from the University of Cape Town, SA, and an MSc (Biological and Chemical Engineering) from the University of Birmingham, UK. She has lectured university courses in bioprocess engineering for over 15 years and has twice received the University of Stellenbosch Rector’s Award for Excellence in Education (2007 and 2010). She heads a research group in bioprocess engineering which focuses on the application of chemical engineering principles in the development and optimization of biological processes. Her research is specifically directed towards fundamental kinetic and mass transfer studies which quantify the principles governing process performance and define process strategies for optimization of the production potential of the biocatalyst.

Affiliations and Expertise

University of Stellenbosch, South Africa


"In a textbook for a graduate or senior undergraduate one-semester course, Clarke…introduces the complementary biotechnology disciplines and their interdependence, emphasizing the application of engineering principles in a life science context. She covers the historical development; microbiology; metabolic macromolecules; molecular biology; carbon metabolism; enzymes as biocatalysts; microbial kinetics during batch, continuous, and fed-batch processes;…", February 2014

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