Autophagy in Mammalian Systems, Part B

Autophagy in Mammalian Systems, Part B

1st Edition - January 27, 2009

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  • Editor: Daniel Klionsky
  • eBook ISBN: 9780080923253
  • Hardcover ISBN: 9780123745477

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This is the companion volume to Daniel Klionsky’s Autophagy: Lower Eukaryotes, which features the basic methods in autophagy covering yeasts and alternative fungi (aspergillus, podospora, magnaporthe). Klionsky is one of the leading authorities in the field. He is the editor-in-chief of Autophagy. The November 2007 issue of Nature Reviews highlighted his article, “Autophagy: From phenomenology to molecular understanding in less than a decade.” He is currently editing guidelines for the field, with 230 contributing authors, that will publish in Autophagy.Particularly in times of stress, like starvation and disease, higher organisms have an internal mechanism in their cells for chewing up and recycling parts of themselves. The process of internal “house cleaning” in the cell is called autophagy – literally self-eating. Breakthroughs in understanding the molecular basis of autophagy came after the cloning of ATG1 (autophagy-related gene 1) in yeast. (To date, 30 additional yeast genes have been identified.) These ATG genes in yeast were the stepping stones to the explosion of research into the molecular analysis of autophagy in higher eukaryotes. In the future, this research will help to design clinical approaches that can turn on autophagy and halt tumor growth.


Researchers in biochemistry, cell and molecular biology, oncology, pharmacology

Table of Contents

  • Chapter 1
    LC3-based assay for monitoring autophagy
    Shunsuke Kimura, Naonobu Fujita, Takeshi Noda and Tamotsu Yoshimori

    Chapter 2
    Analysis of autophagy using GFP-LC3 transgenic mice
    Noboru Mizushima

    Chapter 3
    Photoactivatable GFP
    Jennifer Lippincott-Schwartz and Dale Hailey

    Chapter 4
    Assaying for autophagic protein degradation
    Fred Meijer

    Chapter 5
    Sequestration assays for mammalian autophagy
    Per O. Seglen

    Chapter 6
    Incorporation of monodansylcadaverine (MDC) as an assay to assess autophagy induction and monitoring fusion with a degradative compartment
    Maria Isabel Colombo

    Chapter 7
    The GST-BHMT assay and related assays for autophagy
    Carol A. Mercer and Patrick B. Dennis

    Chapter 8
    Redox and autophagy-ROS as an indicator for autophagic activity
    Ruth Scherz-Shouval and Zvulun Elazar

    Chapter 9
    FACS analysis of autophagy
    Elena Shvets, Ephraim Fass and Zvulun Elazar

    Chapter 10
    Electron microscopy in mammals
    Päivi Ylä-Anttila and Eeva-Liisa Eskelinen

    Chapter 11
    Monitoring mTOR activity
    Ken Inoki

    Chapter 12
    Using p62 as a marker for autophagy.
    Terje Johansen

    Chapter 13
    Cytosolic LC3 ratio as a quantitative index of macroautophagy
    Motoni Kadowaki and Md. Razaul Karim

    Chapter 14
    Pexophagy in mammalian cells
    Junji Ezaki,Takashi Ueno, Eiki Kominami and Masaaki Komatsu

    Chapter 15
    Mitophagy in mammalian cells.
    Ji Zhang and Paul A. Ney

    Chapter 16
    Quantification of WIPI-1/Atg18 puncta formation for assessing mammalian autophagy
    Tassula Proikas-Cezanne and Simon G. Pfisterer

    Chapter 17
    Correlative light and electron microscopy (CLEM) using GFP-LC3 and other autophagosome markers
    Sharon Tooze and Minoo Razi

    Chapter 18
    Nanotechnology assays for autophagy
    Oleksandr Seleverstov

    Chapter 19
    Photoconvertible KAEDA-LC3 for monitoring autophagic flux
    Christoph Goemans and Aviva Tolkovsky

    Chapter 20
    Methods to monitor Chaperone-mediated autophagy
    Ana Maria Cuervo and Susmita Kaushik

    Chapter 21
    Autophagy in response to Salmonella enterica serovar Typhimurium infection
    Cheryl Birmingham and John Brumell

    Chapter 22
    Monitoring autophagy during Mycobacterium tuberculosis infection
    Vojo Deretic

    Chapter 23
    Shigella, Listeria and Streptococcus induction of autophagy
    Michinaga Ogawa,Yuko Yoshikawa, Ichiro Nakagawa,
    Chihiro Sasakawa, and Trinad Chakraborty

    Chapter 24
    Measuring the impact of infection on autophagy in macrophages
    Jean-Francois Dubuisson, Brenda Byrne and Michele Swanson

Product details

  • No. of pages: 560
  • Language: English
  • Copyright: © Academic Press 2009
  • Published: January 27, 2009
  • Imprint: Academic Press
  • eBook ISBN: 9780080923253
  • Hardcover ISBN: 9780123745477

About the Editor

Daniel Klionsky

Affiliations and Expertise

Life Sciences Institute, University of Michigan, USA

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