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Chapter 1 LC3-based assay for monitoring autophagy Shunsuke Kimura, Naonobu Fujita, Takeshi Noda and Tamotsu Yoshimori
Chapter 2 Analysis of autophagy using GFP-LC3 transgenic mice Noboru Mizushima
Chapter 3 Photoactivatable GFP Jennifer Lippincott-Schwartz and Dale Hailey
Chapter 4 Assaying for autophagic protein degradation Fred Meijer
Chapter 5 Sequestration assays for mammalian autophagy Per O. Seglen
Chapter 6 Incorporation of monodansylcadaverine (MDC) as an assay to assess autophagy induction and monitoring fusion with a degradative compartment Maria Isabel Colombo
Chapter 7 The GST-BHMT assay and related assays for autophagy Carol A. Mercer and Patrick B. Dennis
Chapter 8 Redox and autophagy-ROS as an indicator for autophagic activity Ruth Scherz-Shouval and Zvulun Elazar
Chapter 9 FACS analysis of autophagy Elena Shvets, Ephraim Fass and Zvulun Elazar
Chapter 10 Electron microscopy in mammals Päivi Ylä-Anttila and Eeva-Liisa Eskelinen
Chapter 11 Monitoring mTOR activity Ken Inoki
Chapter 12 Using p62 as a marker for autophagy. Terje Johansen
Chapter 13 Cytosolic LC3 ratio as a quantitative index of macroautophagy Motoni Kadowaki and Md. Razaul Karim
Chapter 14 Pexophagy in mammalian cells Junji Ezaki,Takashi Ueno, Eiki Kominami and Masaaki Komatsu
Chapter 15 Mitophagy in mammalian cells. Ji Zhang and Paul A. Ney
Chapter 16 Quantification of WIPI-1/Atg18 puncta formation for assessing mammalian autophagy Tassula Proikas-Cezanne and Simon G. Pfisterer
Chapter 17 Correlative light and electron microscopy (CLEM) using GFP-LC3 and other autophagosome markers Sharon Tooze and Minoo Razi
Chapter 18 Nanotechnology assays for autophagy Oleksandr Seleverstov
Chapter 19 Photoconvertible KAEDA-LC3 for monitoring autophagic flux Christoph Goemans and Aviva Tolkovsky
Chapter 20 Methods to monitor Chaperone-mediated autophagy Ana Maria Cuervo and Susmita Kaushik
Chapter 21 Autophagy in response to Salmonella enterica serovar Typhimurium infection Cheryl Birmingham and John Brumell
Chapter 22 Monitoring autophagy during Mycobacterium tuberculosis infection Vojo Deretic
Chapter 23 Shigella, Listeria and Streptococcus induction of autophagy Michinaga Ogawa,Yuko Yoshikawa, Ichiro Nakagawa, Chihiro Sasakawa, and Trinad Chakraborty
Chapter 24 Measuring the impact of infection on autophagy in macrophages Jean-Francois Dubuisson, Brenda Byrne and Michele Swanson
This is the companion volume to Daniel Klionsky’s Autophagy: Lower Eukaryotes, which features the basic methods in autophagy covering yeasts and alternative fungi (aspergillus, podospora, magnaporthe). Klionsky is one of the leading authorities in the field. He is the editor-in-chief of Autophagy. The November 2007 issue of Nature Reviews highlighted his article, “Autophagy: From phenomenology to molecular understanding in less than a decade.” He is currently editing guidelines for the field, with 230 contributing authors, that will publish in Autophagy.
Particularly in times of stress, like starvation and disease, higher organisms have an internal mechanism in their cells for chewing up and recycling parts of themselves. The process of internal “house cleaning” in the cell is called autophagy – literally self-eating. Breakthroughs in understanding the molecular basis of autophagy came after the cloning of ATG1 (autophagy-related gene 1) in yeast. (To date, 30 additional yeast genes have been identified.) These ATG genes in yeast were the stepping stones to the explosion of research into the molecular analysis of autophagy in higher eukaryotes. In the future, this research will help to design clinical approaches that can turn on autophagy and halt tumor growth.
Researchers in biochemistry, cell and molecular biology, oncology, pharmacology
- No. of pages:
- © Academic Press 2009
- 27th January 2009
- Academic Press
- Hardcover ISBN:
- eBook ISBN:
Life Sciences Institute, University of Michigan, USA
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