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Autophagy in Mammalian Systems, Part B - 1st Edition - ISBN: 9780123745477, 9780080923253

Autophagy in Mammalian Systems, Part B, Volume 452

1st Edition

Editor: Daniel Klionsky
Hardcover ISBN: 9780123745477
eBook ISBN: 9780080923253
Imprint: Academic Press
Published Date: 27th January 2009
Page Count: 560
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Table of Contents

Chapter 1 LC3-based assay for monitoring autophagy Shunsuke Kimura, Naonobu Fujita, Takeshi Noda and Tamotsu Yoshimori

Chapter 2 Analysis of autophagy using GFP-LC3 transgenic mice Noboru Mizushima

Chapter 3 Photoactivatable GFP Jennifer Lippincott-Schwartz and Dale Hailey

Chapter 4 Assaying for autophagic protein degradation Fred Meijer

Chapter 5 Sequestration assays for mammalian autophagy Per O. Seglen

Chapter 6 Incorporation of monodansylcadaverine (MDC) as an assay to assess autophagy induction and monitoring fusion with a degradative compartment Maria Isabel Colombo

Chapter 7 The GST-BHMT assay and related assays for autophagy Carol A. Mercer and Patrick B. Dennis

Chapter 8 Redox and autophagy-ROS as an indicator for autophagic activity Ruth Scherz-Shouval and Zvulun Elazar

Chapter 9 FACS analysis of autophagy Elena Shvets, Ephraim Fass and Zvulun Elazar

Chapter 10 Electron microscopy in mammals Päivi Ylä-Anttila and Eeva-Liisa Eskelinen

Chapter 11 Monitoring mTOR activity Ken Inoki

Chapter 12 Using p62 as a marker for autophagy. Terje Johansen

Chapter 13 Cytosolic LC3 ratio as a quantitative index of macroautophagy Motoni Kadowaki and Md. Razaul Karim

Chapter 14 Pexophagy in mammalian cells Junji Ezaki,Takashi Ueno, Eiki Kominami and Masaaki Komatsu

Chapter 15 Mitophagy in mammalian cells. Ji Zhang and Paul A. Ney

Chapter 16 Quantification of WIPI-1/Atg18 puncta formation for assessing mammalian autophagy Tassula Proikas-Cezanne and Simon G. Pfisterer

Chapter 17 Correlative light and electron microscopy (CLEM) using GFP-LC3 and other autophagosome markers Sharon Tooze and Minoo Razi

Chapter 18 Nanotechnology assays for autophagy Oleksandr Seleverstov

Chapter 19 Photoconvertible KAEDA-LC3 for monitoring autophagic flux Christoph Goemans and Aviva Tolkovsky

Chapter 20 Methods to monitor Chaperone-mediated autophagy Ana Maria Cuervo and Susmita Kaushik

Chapter 21 Autophagy in response to Salmonella enterica serovar Typhimurium infection Cheryl Birmingham and John Brumell

Chapter 22 Monitoring autophagy during Mycobacterium tuberculosis infection Vojo Deretic

Chapter 23 Shigella, Listeria and Streptococcus induction of autophagy Michinaga Ogawa,Yuko Yoshikawa, Ichiro Nakagawa, Chihiro Sasakawa, and Trinad Chakraborty

Chapter 24 Measuring the impact of infection on autophagy in macrophages Jean-Francois Dubuisson, Brenda Byrne and Michele Swanson


This is the companion volume to Daniel Klionsky’s Autophagy: Lower Eukaryotes, which features the basic methods in autophagy covering yeasts and alternative fungi (aspergillus, podospora, magnaporthe). Klionsky is one of the leading authorities in the field. He is the editor-in-chief of Autophagy. The November 2007 issue of Nature Reviews highlighted his article, “Autophagy: From phenomenology to molecular understanding in less than a decade.” He is currently editing guidelines for the field, with 230 contributing authors, that will publish in Autophagy.

Particularly in times of stress, like starvation and disease, higher organisms have an internal mechanism in their cells for chewing up and recycling parts of themselves. The process of internal “house cleaning” in the cell is called autophagy – literally self-eating. Breakthroughs in understanding the molecular basis of autophagy came after the cloning of ATG1 (autophagy-related gene 1) in yeast. (To date, 30 additional yeast genes have been identified.) These ATG genes in yeast were the stepping stones to the explosion of research into the molecular analysis of autophagy in higher eukaryotes. In the future, this research will help to design clinical approaches that can turn on autophagy and halt tumor growth.


Researchers in biochemistry, cell and molecular biology, oncology, pharmacology


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© Academic Press 2009
27th January 2009
Academic Press
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About the Editor

Daniel Klionsky

Affiliations and Expertise

Life Sciences Institute, University of Michigan, USA