Mechanisms of Eukaryotic DNA Recombination

Mechanisms of Eukaryotic DNA Recombination

1st Edition - June 15, 1992

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  • Editors: Max E Gottesman, Henry J. Vogel
  • eBook ISBN: 9781483274089

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Description

Mechanisms of Eukaryotic DNA Recombination is a collection of papers that discusses advances in eukaryotic genetic recombination. Papers address issues in eukaryotic genetic recombination, particularly DNA integration in mammalian genomes, genetic recombination in Drosophila or Caenorhabditis; the manipulation of the mouse genome; genome organization; and genetic recombination in protozoa. One paper discusses chromatid interactions during intrachromosomal recombination in mammalian cells, namely, intrachromatid and sister chromatid. Another paper analyzes the implication for chromosomal recombination and gene targeting; results on extrachromosomal recombination show that circles are inefficient substrates for recombination even if only one of two substrates in an intermolecular reaction is circular. One author discusses the genetics and molecular biology of recombination, citing the work of Watson and Crick, stating that crossing-over occurs between genes (not within them). He also explains that the formation and resolution of recombination intermediaries depend on enzyme or other proteins. This book will prove invaluable to cellular biologists, microbiologists, and researchers engaged in genetics and general biology.

Table of Contents


  • Preface

    Part I Targeted DNA Integration in Mammalian Cells

    1 Chromatid Interactions during Intrachromosomal Recombination in Mammalian Cells

    Introduction

    Results and Discussion

    Conclusion

    References

    2 Studies on Extrachromosomal Homologous Recombination in Mammalian Cells: Implications for Chromosomal Recombination and Gene Targeting

    Introduction

    Results

    Discussion

    References

    3 Homologous Recombination in Embryonic Stem Cells as a Means to Generate Mice with Defined Mutations

    Introduction

    General Strategy

    Microinjection of Mutated Hox-1.1 Fragments into Embryonic Stem Cells

    Cloning of Homologous Recombined Cell Lines

    Generation of Chimeric Animals with Homologously Recombined Cells

    Discussion

    References

    4 Identification and Targeted Mutation of Developmental Genes in Mouse Embryonic Stem Cells

    Introduction

    Embryonic Stem Cell Technology

    Identification of New Domains of Gene Action in Developing Embryos

    Targeted Mutation of Cloned Genes

    Problems and Prospects

    References

    Part II DNA Insertion Phenomena

    5 Developmental Mutations Generated by Retroviral Insertional Mutagenesis

    Introduction

    Results and Discussion

    Summary and Conclusion

    References

    6 Transfer of Yeast Artificial Chromosomes into Cultured Cells: A New Method for Manipulating the Mammalian Genome

    Introduction

    Results

    Conclusions and Future Prospects

    References

    7 Sex-Dependent de Novo Methylation of the Transgene and Its Insertional Locus on Mouse Chromosome

    Introduction

    Results

    References

    8 A Role for Transcription in Antibody Switch Recombination

    Introduction

    Results and Discussion

    Model for the Mechanism of Switch Recombination

    References

    Part III Genetic Recombination in Drosophila and Caenorhabditis

    9 Genetic and Molecular Studies of a Simple Meiotic System

    Introduction

    Partner Choice and the Nature of Distributive Pairing

    The Segregation of Nonexchange Chromosomes

    Summary

    References

    10 Diversity among DrosophUa Transposable Elements and in Their Effects on Gene Expression

    Introduction

    Classes of Transposable Elements

    Regulation of Element Activity

    Alteration of Gene Expression

    Summary

    References

    11 Meiotic Recombination in Caenorhabditis elegans

    Introduction

    Homolog Recognition

    Pairing and Recombination

    Frequency of Recombination

    Distribution of Recombination Events along the Chromosome

    Disjunction

    Summary

    References

    Part Iv Genetic Recombination in Yeasts and Ustilago

    12 Saccharomyces Cerevisiae Proteins That Promote Hybrid DNA Formation In Vitro

    Introduction

    The Strand-Exchange Protein, SEP1, from Saccharomyces Cerevisiae

    Additional Factors Involved in Hybrid DNA Formation In Vitro

    Mr 34,000 Single-Stranded DNA-Binding Protein [Fragment of yRPA (yRFA)]

    Stimulatory Factor 1

    Discussion

    References

    13 Gap Repair in Vitro Catalyzed by Cell-Free Extracts from Yeast

    Introduction

    Results and Discussion

    References

    14 Meiotic Recombination in Schizosaccharomyces Pombe: Genes, Enzymes, and Sites

    Introduction

    Genes

    Enzymes

    Sites

    Summary

    References

    15 Genetics and Molecular Biology of Recombination

    Introduction

    Recombination and Heteroduplex DNA

    Mutants Defective in Recombination

    Recombination Proteins

    Molecular Analysis

    References

    Part V Genetic Recombination in Trypanosomes and Plasmodium

    16 Chromosome and Telomere Structure in Trypanosoma Brucei

    Introduction

    Organization of the Trypanosoma brucei Genome; Presence of Chromosome Homologs

    Minichromosome Structure

    Concluding Remarks

    References

    17 Use of DNA Sequence Homology and Pseudogenes for the Construction of Active Variable Surface Antigen Genes in Trypanosoma equiperdum: How Ordered Expression Is Established

    Introduction

    Early- and Late-Appearing Variable Surface Glycoproteins

    Early Genes Have Complete Silent Copies, whereas Late Silent Copy Genes Are Defective

    The Use of DNA Sequence Homology to Generate Ordered Expression of the Late-Appearing Variable Cell Surface Glycoprotein Genes

    5-3 Joining

    A Possible Molecular Mechanism for Late-Variable Surface Glycoprotein Expression-Linked Copy Formation

    Why Pseudogenes?

    References

    18 Chromosomal Size Variations in Plasmodium fcdeiparum

    Introduction

    Intragenic Recombination in a Major Antigen of Plasmodium Falciparum

    Size Polymorphisms in Plasmodium Falciparum Chromosomes

    Chromosome Size Polymorphisms Generated by Deletion of Subtelomeric Repeats

    Deletions Involving Coding Sequences

    The Multidrug Resistance Gene oi Plasmodium Falciparum and Chromosome Size Polymorphisms

    Conclusion

    References

    Index


Product details

  • No. of pages: 228
  • Language: English
  • Copyright: © Academic Press 1992
  • Published: June 15, 1992
  • Imprint: Academic Press
  • eBook ISBN: 9781483274089

About the Editors

Max E Gottesman

Henry J. Vogel

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