The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences.


Biochmemists, biophysicists, cell biologists, molecular biologists, geneticists, developmental biologists

Table of Contents

Dynamic GFP Sensors for High-Content Analysis of the Cell Cycle High Content Fluorescence-based Screening for Epigenetic Modulators Development of Assays for Nuclear Receptor Modulators Using Fluorescently Tagged Proteins The Ligand-Independent Translocation Assay: An Enabling Technology for Screening Orphan GPCRs by Arrestin Recruitment High Content Screening of Known G Protein-Coupled Receptors by Arrestin Translocation Cell Imaging Assays for G protein-coupled Receptor Internalization: Application to High Throughput Screening High throughput confocal microscopy for beta arrestin-GFP translocation GPCR assays using the Evotec Opera G-protein coupled receptor internalization assays in the High Content Screening Format Screening for Activators of the Wnt/Fzd Pathway by Automated Fluorescent Microscopy A live-cell, image based approach to understanding the enzymology and pharmacology of 2-bromopalmitate and palmitoylation Quantitative single cell studies of nuclear receptor function using high-resolution HTM and image analyses Tracking the motion of individual proteins with semiconductor quantum dots Development and Application of Automatic High Resolution Light Microscopy for Cell Based Screens Adenoviral Sensors for High-Content Cellular Analysis Cell based assays in primary endothelial cells to study multiple steps in inflammation Development and Implementation of Multiplexed Cell-Based Imaging Assays High throughput screening for modulators of stem cell differentiation High Content Kinetic Calcium Imaging in Drug Sensitive and Drug Resistant Human Breast Cancer Cells Measurement and Analysis of Calcium Signaling in Heterogeneous Cell Cultures Mulipliex Analysis of Inflammatory Signalling Pathways using a High Content Ima


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© 2006
Academic Press
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