Laboratory Fish in Biomedical Research

Laboratory Fish in Biomedical Research

Biology, Husbandry and Research Applications for Zebrafish, Medaka, Killifish, Cavefish, Stickleback, Goldfish and Danionella Translucida

1st Edition - August 28, 2021

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  • Editors: Livia d'Angelo, Paolo de Girolamo
  • Paperback ISBN: 9780128210994
  • eBook ISBN: 9780128212455

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Fish, and particularly zebrafish, have become the fastest-growing segment of the research population. They offer several advantages, in terms of biology and technologies to apply, and thus are employed in numerous research fields. Laboratory Fish in Biomedical Research: Biology, Husbandry and Research Applications for Zebrafish, Medaka, Killifish, Swordtail Fish, Cavefish, Stickleback, Goldfish and Danionella Translucida addresses the relevant and increasing need to collect cutting-edge knowledge on husbandry, maintenance, welfare and experimental protocols of the most common freshwater species under standard laboratory conditions.

Key Features

  • Provides husbandry and management protocols, devices and water systems
  • Shows strength and weakness of breeding
  • Explores potential scientific applications and experimental protocols. with regards to the most used freshwater fish used for scientific purposes


Academies, Institutes of research, Foundations involved in education and training in the field of laboratory animal sciences (LAS), National and International Associations/Scientific Societies involved in the field of LAS. Companies (i) producing laboratory aquatics infrastructures and devices, (ii) offering services for health monitoring. Scientists, Technicians, Laboratory managers, Animal caretaker, Veterinarians

Table of Contents

  • Cover image
  • Title page
  • Table of Contents
  • Copyright
  • Contributors
  • Fish as model systems
  • Section 1. Zebrafish (Danio rerio)
  • Chapter 1. Biology and research applications
  • The biology of the zebrafish
  • The history of the zebrafish as laboratory model
  • The zebrafish as a model for development and pathology
  • Chapter 2. Housing and maintenance of zebrafish, new technologies in laboratory aquatic systems and considerations for facility design
  • Introduction to the model
  • Aquatic concepts
  • Establishing biofiltration
  • Husbandry
  • Chapter 3. Breeding and larviculture of zebrafish (Danio rerio)
  • Introduction
  • Should we mimic nature, or should we ignore it in laboratory breeding and husbandry of zebrafish: theoretical considerations from the perspective of experimental biology
  • Zebrafish in nature
  • Preparing zebrafish for breeding
  • The breeding tank
  • Raising zebrafish juveniles is not as easy as often stated
  • Hatching the eggs and caring for the larvae
  • Caring for young juveniles
  • Standardization of breeding and larviculture practices: the question of replicability and reproducibility
  • Chapter 4. Health monitoring, disease, and clinical pathology
  • Introduction
  • Controlling and monitoring population health
  • Pathogens and diseases to monitor
  • Fish and environmental samples
  • Diagnostic assays
  • Determining the number of samples
  • Introduction of zebrafish colonies and biosecurity
  • Conclusion
  • Chapter 5. The welfare of zebrafish
  • Introduction
  • Measuring welfare in zebrafish
  • Husbandry
  • Refinements in regulated procedures
  • Conclusion: the state of zebrafish welfare today
  • Chapter 6. Analgesia, anesthesia, and euthanasia in zebrafish
  • Analgesia
  • Anesthesia
  • Euthanasia
  • Conclusions
  • Chapter 7. Transgenesis, mutagenesis, knockdown, and genetic colony management
  • Introduction
  • Ethical issues in genetic manipulation of laboratory fish
  • Generation and management of morphants and CRISPants
  • Conclusions
  • Chapter 8. Sperm cryopreservation, in vitro fertilization, and embryo freezing
  • Introduction
  • Materials and methods
  • Conclusion
  • Section 2. Other small freshwater fish
  • Chapter 9. Medaka as a model teleost: characteristics and approaches of genetic modification
  • Medaka
  • Basic approaches of generation of transgenic/knockout medaka
  • Introduction of new transgenic methods
  • Chapter 10. Integrated analyses using medaka as a powerful model animal toward understanding various aspects of reproductive regulation
  • Medaka as a powerful model animal for research fields of reproduction
  • Mechanisms of reproductive systems clarified by histological and physiological analyses
  • Histological studies using medaka
  • The present scheme of HPG axis regulation clarified by multidisciplinary analyses in vertebrates
  • Endocrine studies using medaka
  • Neurophysiological studies using medaka: patch clamp recording and calcium imaging
  • Medaka provides clues to the understanding of general mechanisms of reproduction in teleosts and even in vertebrates
  • Chapter 11. The African turquoise killifish (Nothobranchius furzeri): biology and research applications
  • Introduction
  • Large-scale husbandry (including water systems and water parameters)
  • Breeding strategies for genome engineering, including egg collection and incubation
  • Efficient genome engineering approaches
  • Fish dissection
  • The African turquoise killifish: available toolbox
  • Summary and future perspectives
  • Materials
  • Chapter 12. Challenges in keeping annual killifish
  • Introduction
  • Available strains and species
  • Housing and breeding
  • Embryo development
  • Diet
  • Diseases
  • Summary of the challenges
  • Chapter 13. Mexican tetra (Astyanax mexicanus): biology, husbandry, and experimental protocols
  • Biology and natural history
  • Husbandry
  • Colony management
  • Animal welfare and health management
  • Procedures
  • Chapter 14. The housing, care, and use of a laboratory three-spined stickleback colony
  • Introduction
  • Supply, quarantine, and disinfection
  • Basic husbandry
  • Establishing and maintaining a successful colony
  • Procedures
  • Conclusion
  • Chapter 15. Goldfish (Carassius auratus): biology, husbandry, and research applications
  • Taxonomy and phylogeny
  • Morphology/anatomy
  • Geography and natural habitat
  • Behavior
  • Husbandry
  • Goldfish in biomedical research
  • Chapter 16. Danionella translucida, a tankful of new opportunities
  • Biology and ecology of Danionella translucida
  • Neuroscience
  • Development and evolution
  • Animal husbandry: early larval stages
  • Animal husbandry: early stages to adulthood
  • Breeding
  • Supplementary data
  • Supplementary data
  • Section 3. Databases
  • Chapter 17. Fish inventory databases
  • Introduction
  • Fish characterization and grouping
  • Record browsing and spatial tracking
  • Performing actions and monitoring parameters
  • Statistics and reporting
  • Advanced features
  • Examples of current solutions
  • Future of fish databases
  • Index

Product details

  • No. of pages: 474
  • Language: English
  • Copyright: © Academic Press 2021
  • Published: August 28, 2021
  • Imprint: Academic Press
  • Paperback ISBN: 9780128210994
  • eBook ISBN: 9780128212455

About the Editors

Livia d'Angelo

Livia D’Angelo is veterinarian by background, she is researcher at University of Naples. Her research activities are mainly focused on the neurobiology of two freshwater fish, zebrafish and the turquoise killifish. She is also deeply involved in the laboratory animal sciences field, with respect to husbandry and management of aquatic research organisms.

Affiliations and Expertise

University of Naples Federico II, Napoli, Italy

Paolo de Girolamo

Paolo de Girolamo is a veterinarian by background, he is full Professor at University of Naples, and he is in charge of Chief of Veterinary Officer and Ethical Animal Care and Use Committee. His research interests have been since long addressed to the neurobiology and gut-brain axis interplay in teleostean fish (goldfish, zebrafish, turquoise killifish etc.).

Affiliations and Expertise

University of Naples Federico II, Napoli, Italy

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