Guide to Protein Purification, Volume 463

2nd Edition

Editors: Richard Burgess Murray Deutscher
Hardcover ISBN: 9780123745361
eBook ISBN: 9780080923178
Imprint: Academic Press
Published Date: 17th November 2009
Page Count: 900
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Table of Contents

  1. Why Purify Enzymes?
  2. Murray Deutscher and Arthur Kornberg

    Section I: Developing Purification Procedures

  3. Strategies and Considerations for Protein Purifications
  4. Stuart Linn

  5. Use of Bioinformatics in Planning a Protein Purification
  6. Richard R. Burgess

  7. Preparing A Purification Summary Table
  8. Richard R. Burgess

    Section II: General Methods for Handling Proteins and Enzymes

  9. Setting Up A Laboratory
  10. Murray P. Deutscher

  11. Buffers: Principle and Practice
  12. Vincent S. Stoll and John S. Blanchard

  13. Measurement of Enzyme Activity
  14. T. K. Harris and M. M. Keshwani

  15. Quantitation of Protein
  16. James E. Noble and Marc J. A. Bailey

  17. Concentration of Proteins and Removal of Solutes
  18. Jonathan K. Romero, David R. H. Evans and Matthew Westoby

  19. Maintaining Protein Stability
  20. Murray P. Deutscher

    Section III: Recombinant Protein Expression and Purification

  21. Selecting an Appropriate Method for Expressing a Recombinant Protein
  22. William H. Brondyk

  23. Bacterial Systems for Production of Heterologous Proteins
  24. Frank R. Collart, Ashley M. Frank and Sarah Zerbs

  25. Expression in the Yeast Pichia Pastoris
  26. James M. Cregg, Ilya Tolstorukov, Anasua Kusari, Jay Sunga, Knut Madden and Thomas Chappell

  27. Baculovirus-Insect Cell Expression Systems
  28. Donald L. Jarvis

  29. Recombinant Protein Production By Transient Gene Transfer Into Mammalian Cells
  30. Sabine Geisse and Cornelia Fux

  31. Tagging F


The 2e of this classic Guide to Protein Purification provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition’s publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology and biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This "Guide to" gives imminently practical advice to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while presents a comprehensive overview of the field today.

Key Features

  • Gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines, including biochemistry, genetics, oncology, pharmacology, dermatology and immunology
  • Assembles chapters on both common and less common relevant techniques
  • Provides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming process


Researchers, professors, and students of biochemistry, molecular biology and cell biology


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© Academic Press 2009
Academic Press
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About the Editors

Richard Burgess Editor

Murray Deutscher Editor

Affiliations and Expertise

University of Connecticut Health Center, Farmington, Connecticut, U.S.A.