Eukaryotic RNases and their Partners in RNA Degradation and Biogenesis - 1st Edition - ISBN: 9780124047402, 9780124047457

Eukaryotic RNases and their Partners in RNA Degradation and Biogenesis, Volume 31

1st Edition

Part A

Serial Volume Editors: Guillaume Chanfreau Fuyuhiko Tamanoi
eBook ISBN: 9780124047457
Hardcover ISBN: 9780124047402
Imprint: Academic Press
Published Date: 16th October 2012
Page Count: 278
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Table of Contents


Chapter One. Biochemistry and Function of RNA Exosomes

1. Introduction

2. Cellular Functions of the Exosome

3. Composition of Exosomes and Their Related Complexes

4. Exosome Cofactors

5. Concluding Remarks


Chapter Two. Plant Exosomes and Cofactors

1. Introduction

2. Composition of the Plant Exosome Core Complex

3. Exosome-Related Activities and Cofactors in Plants

4. Impact of Exosome-Mediated Degradation in Plants

5. Final Remarks


Chapter Three. Structure and Activities of the Eukaryotic RNA Exosome

1. Introduction

2. Global Architecture of the Eukaryotic Exosome Core

3. RNase PH-Like Domains Comprise a PH-Like Ring in Eukaryotic Exosomes

4. S1 and KH Domains Cap the PH-Like Ring

5. RRP44, a Hydrolytic Endoribonuclease and Processive Exoribonuclease

6. RRP44 and the 10-Component Exosome

7. RRP6, a Eukaryotic Exosome Subunit with Distributive Hydrolytic Activities

8. RRP44, RRP6, and the 11-Component Nuclear Exosome

9. Conclusions


Chapter Four. TRAMP Stimulation of Exosome

1. TRAMP Is a Cofactor of Nuclear Exosome

2. RNA Substrate Repertoire of TRAMP

3. TRAMP Biochemistry and Structure

4. TRAMP Complex in Activation of the Exosome

5. TRAMP Complexes in Different Organisms


Chapter Five. XRN1: A Major 5′ to 3′ Exoribonuclease in Eukaryotic Cells

1. Introduction

2. XRN1 in mRNA Decay

3. XRN1 in mRNA Quality Control

4. XRN1 in miRNA-Mediated Decay

5. XRN1 in siRNA-Mediated Decay

6. Localization of XRN1 in Cells

7. XRN1 in lncRNA Decay

8. XRN1 in tRNA Quality Control

9. XRN1 in rRNA and snoRNA Processing

10. Regulation of XRN1 Activity

11. Summary


Chapter Six. Structures of 5′–3′ Exoribonucleases

1. Introduction

2. Crystal Structure of RAT1/XRN2

3. Crystal Structures of XRN1

4. Structural Homologs of XRNs

5. Active Site of XRNs

6. Structure of the RAT1–RAI1 Complex

7. Structure of RAI1/DOM3Z

8. Perspectives


Chapter Seven. Rat1 and Xrn2: The Diverse Functions of the Nuclear Rat1/Xrn2 Exonuclease

1. Introduction

2. The Life Story of RAT1/XRN2

3. Sequence and Structural Data

4. One Protein, Many Functions

5. Overlapping Functions of RAT1 and XRN1

6. XRN2 as a Silencing Suppressor

7. Perspectives


Chapter Eight. Normal and Aberrantly Capped mRNA Decapping

1. Introduction

2. mRNA-Decapping Proteins in the Exonucleolytic Pathway of mRNA Decay

3. Presence of an Aberrant Cap-Decapping Protein in S. cerevisiae

4. Additional Potential Functions of RAI1

5. Future Directions


Chapter Nine. Activity and Function of Deadenylases

1. Introduction

2. The Poly(A) Nuclease (PAN)

3. The Poly(A)-Specific Ribonuclease

4. The CCR4–NOT Complex


Chapter Ten. The Diverse Functions of Fungal RNase III Enzymes in RNA Metabolism

1. Introduction

2. Phylogenetic Distribution and Conservation of RNase III Enzymes in Fungi

3. Ribosomal RNA Processing and RNA Polymerase I Transcriptional Termination

4. Small Nuclear RNAs Processing

5. Functions in Small Nucleolar RNAs Processing

6. (Pre)-mRNA Surveillance, Degradation, and Regulation

7. RNA Polymerase II Termination

8. Conclusions and Perspectives


Author Index

Subject Index


This special issue of The Enzymes is targeted towards researchers in biochemistry, molecular and cell biology, pharmacology, and cancer. This volume discusses Eukaryotic RNases and their partners in RNA degradation and biogenesis.

Key Features

  • Contributions from leading authorities
  • Informs and updates on all the latest developments in the field


Researchers in biochemistry, molecular and cell biology, pharmacology, and cancer


No. of pages:
© Academic Press 2012
Academic Press
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Hardcover ISBN:

About the Serial Volume Editors

Guillaume Chanfreau Serial Volume Editor

Dr Guillaume Chanfreau obtained his Bachelor Degree from Université Claude Bernard Lyon and ENS Lyon and his PhD in Microbiology from Université Paris VI. During his PhD, mentored by Alain Jacquier, he characterized the mechanism of splicing of group II intron ribozymes. Dr Chanfreau then completed postdoctoral training at UCSF with Christine Guthrie, where he identified novel factors involved in 3’-end processing of mRNAs and small RNAs. After this postdoctoral training, he was recruited at UCLA as a professor in the Department of Chemistry and Biochemistry where he teaches a Biochemistry upper division course focused on DNA, RNA and protein synthesis. At UCLA, Dr Chanfreau has developed a research program focused on understanding the mechanisms of RNA degradation and RNA processing, and how these processes contribute to regulate gene expression.

Affiliations and Expertise

University of California, Los Angeles, USA

Fuyuhiko Tamanoi Serial Volume Editor

Fuyu Tamanoi is a biochemist who has served on the UCLA School of Medicine and UCLA College faculty since he joined the Department of Microbiology, Immunology & Molecular Genetics in 1993. He became a full professor in 1997. Since 1996, he has been a Director of Signal Transduction Program Area at Jonsson Comprehensive Cancer Center. Dr. Tamanoi earned his B.S. and M.S. in Biochemistry at the University of Tokyo. He received PhD in Molecular Biology at Nagoya University in 1977. He was a postdoctoral fellow at Harvard Medical School, where he worked on bacteriophage DNA replication. From 1980 to 1985, he was a senior staff investigator at Cold Spring Harbor Laboratory, where he worked on adenovirus DNA replication. From 1985 to 1993, he was an Assistant Professor and then Associate Professor at the University of Chicago, where he initiated studies on lipid modification of the Ras family proteins. His laboratory research centers on signal transduction and signal transduction inhibitors. He is currently exploring ways to deliver signal transduction inhibitors using nanoparticles.

Affiliations and Expertise

Professor and Vice Chair, Dept. of Microbiology, Immunology & Molecular Genetics, University of California, Los Angeles, USA Director, Signal Transduction Program Area, Jonsson Comprehensive Cancer Center, USA