Zone Electrophoresis - 4th Edition - ISBN: 9780815178408, 9781483279916

Zone Electrophoresis

4th Edition

Editors: Ivor Smith
eBook ISBN: 9781483279916
Imprint: Butterworth-Heinemann
Published Date: 1st January 1976
Page Count: 496
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Description

Chromatographic & Electrophoretic Techniques, Fourth Edition, Volume II: Zone Electrophoresis presents the methods of zone electrophoresis. This book discusses all the techniques that can be applied routinely.

Organized into 17 chapters, this volume begins with an overview of the fundamental principle of zone electrophoresis. This text then examines the apparatus in common use for paper electrophoresis and discusses paper electrophoresis at low voltages. Other chapters describe the apparatus that combines several advantages resulting from high voltage paper electrophoresis and thin-layer chromatography. This book discusses as well the advantages of cellulose acetate membrane filter paper as a supporting medium for zone electrophoresis. The final chapter deals with preparative acrylamide gel electrophoresis on a small scale, which may involve either continuous electrophoretic elution or extraction of the RNA from zones cut out of the gel.

This book is a valuable resource for biochemists, clinical chemists, clinicians, technicians, biophysicists, pathologists, and medical researchers.

Table of Contents


Editor and Authors

Preface

1. General Principles of Zone Electrophoresis

2. Paper Electrophoresis at Low Voltage

3. High Voltage Paper Electrophoresis

4 . Thin Layer Electrophoresis

5. Cellulose Acetate Electrophoresis and Immuno-Diffusion Techniques

6. Agar Gel Electrophoresis and Immuno-Electrophoresis

7. Starch Gel Electrophoresis

8. Separation and Visualization of Enzymes on Gels

9. Acrylamide Gel Disc Electrophoresis

Techniques of Disc Electrophoresis

Protein Patterns in Human Serum; Interpretation

10. Concentration Gradient Polyacrylamide Gel Electrophoresis

11. Isoelectric Focusing of Proteins

12. Electrophoresis of Proteins on SDS Polyacrylamide Gels

13. Quantitative Immunoelectrophoresis

14. Preparative Block Electrophoresis

15. Preparative Acrylamide Disc Electrophoresis

Section 1

Section 2

16. Models for Macromolecules, Nucleic Acids and Proteins

17. Electrophoresis of RNA in Polyacrylamide and Agarose Gels

Index

Details

No. of pages:
496
Language:
English
Copyright:
© Butterworth-Heinemann 1976
Published:
Imprint:
Butterworth-Heinemann
eBook ISBN:
9781483279916

About the Editor

Ivor Smith