Yeast Gene Analysis

Yeast Gene Analysis

1st Edition - April 15, 1998

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  • Editor: Mick Tuite
  • eBook ISBN: 9780080860558

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Established almost 30 years ago, Methods in Microbiology is the most prestigious series devoted to techniques and methodology in the field. Now totally revamped, revitalized, with a new format and expanded scope, Methods in Microbiology will continue to provide you with tried and tested, cutting edge protocols to directly benefit your research.

Key Features

Focuses on the methods most useful for the functional analysis of yeast genes
Allows researcher to identify which strategy to use without having to wade through numerous recipes
Includes techniques for mutagenesis, transcript analysis, transposon tagging, and use of reporter genes
Describes the use of tools for studying post-translational modifications in yeast
Includes useful appendices with handy basic yeast recipes and WWW addresses


Microbiologists, biotechnologists, and molecular biologists

Table of Contents

  • S.G. Oliver,<$> Introduction to Functional Analysis of the Yeast Genome. E.J. Louis,<$> Whole Chromosome Analysis. H.W. Mewes, D. Frishman, A. Zollner, and K. Heumann,<$> The Bioinformatics of the Yeast Genome. R.D.Gietz and R.A. Woods,<$> Transformation of Yeast by the Lithium Acetate/Single-Stranded Carrier DNA/PEG Method. A. Wach, A. Brachat, C. Rebischung, S. Steiner, K. Pokorni, S. te Heesen, and P. Philippsen,<$> PCR-based Gene Targeting in Saccharomyces cerevisiae<$>. M.J.R. Stark,<$> Studying Essential Genes–Generating and Using Promoter Fusions and Conditional Alleles. D.J. Garfinkel, M.J. Curcio, and V. Smith,<$> Ty Mutagenesis. A.J.P. Brown, L.M. Furness, and D. Bailey,<$> TranscriptAnalysis. N. Koloteva, J.M.X. Hughes, and J.E.G. McCarthy,<$> Reporter Genes and Their Use in Studying Yeast Gene Expression. P. Ross-Macdonald, A. Sheehan, C. Friddle, G.S. Roeder, and M. Snyder,<$> Transposon Tagging I: A Novel System for Monitoring Protein Production, Function and Localization. M. Bolotin-Fukuhara,<$> Transposon Tagging II: Exploration of Gene Function and Regulatory Networks in Yeast with the Mini-Mu Transposon. I.M. Hagan and C.J. Stirling,<$> Immunological Approaches tothe Study of Protein Localisation in Yeast. F.M. Klis, A.F.J. Ram, R.C. Montijn, J.C. Kapteyn, L.H.P. Caro, J.H. Vossen, M.A.A. Van Berkel, S.S.C. Brekelmans, and H. Van den Ende,<$> Posttranslational Modifications of Secretory Proteins. M.J.R. Stark,<$> Studying Post-Translational Modifications in Yeast–Intracellular Proteins. J. Rosamond,<$> Two-Hybrid Analysis of Protein–Protein Interactions in Yeast. J. de la Cruz, M.C. Daugeron, and P. Linder,<$> 'Smart Genetic Screens. B. Teusink, F. Baganz, H.V. Westerhoff, and S.G. Oliver,<$> Metabolic Control Analysis as a Tool in the Elucidation of the Function of Novel Genes. W.H. Mager, K. Maurer, and P.W. Piper,<$> Identifying Stress Genes. M.F. Tuite, I. Stansfield, and R.J. Planta,<$> Identifying Genes Encoding Components of the Protein Synthesis Machinery of the Yeast Saccharomyces cerevisiae<$>. M. Molina, H. Martan, M. Sanchez, and C. Nombela,<$> MAP Kinase-Mediated Signal Transduction Pathways. P.T. Magee,<$> TheAnalysis of the Candida albicans<$> Genome. P.A. Moore,<$> Applications of Yeast in the Discovery of Human Gene Function. K.D. Entian and P. Kitter,<$> Yeast Mutant and Plasmid Collections. A. Brown and M.F. Tuite,<$> Yeast Gene Analysis in the 21st Century. Appendices:<$> R.J. Planta, I. Stansfield, and M.F. Tuite,<$> The Ribosomal Protein Genes of Saccharomyces cerevisiae. A.J.P. Brown and M.F. Tuite,<$> Yeast Growth. A.J.P. Brown,<$> Useful World Wide Web Addresses for YeastResearchers. M.F. Tuite,<$> Nomenclature for Yeast Genes and Proteins. Subject Index.

Product details

  • No. of pages: 502
  • Language: English
  • Copyright: © Academic Press 1998
  • Published: April 15, 1998
  • Imprint: Academic Press
  • eBook ISBN: 9780080860558

About the Serial Volume Editor

Mick Tuite

Mick Tuite is a Professor of Molecular Biology in the Department of Biosciences at the University of Kent, UK He has published over 100 research papers and reviews in the area of yeast molecular biology and genetics and is a member of the editorial board of the journals Yeast and Molecular Microbiology.

Affiliations and Expertise

University of Kent, Canterbury, U.K.

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