Yeast Gene Analysis

Yeast Gene Analysis

2nd Edition - May 25, 2007

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  • Editor: Ian Stansfield
  • Hardcover ISBN: 9780123694782
  • eBook ISBN: 9780080521787

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Description

Focusing on Saccharomyces cerevisiae, the second edition of Yeast Gene Analysis represents a major reworking of the original edition, with many completely new chapters and major revisions to all previous chapters. Originally published shortly after completion of the yeast genome sequence, the new edition covers many of the major genome-wide strategies that have been developed since then such as microarray analysis of transcription, synthetic gene array studies, protein microarrays and chemical genetic approaches. It represents a valuable resource for any research laboratory using budding yeast as their experimental system in which to identify new yeast gene functions. The chapters are written in a readable style with useful background information, technical tips and specific experimental protocols included as appropriate, enabling both the novice and the experienced yeast researcher to adopt new procedures with confidence.

Key Features

  • New chapters on: Strain construction; genome-wide two-hybrid approaches; use of microarrays for transcript analysis; real-time analysis of chromosome behaviour and FRET; synthetic gene array technology and protein arrays; chemical genomics and yeast prions; RNA gene analysis and mitochondrial gene function analysis; phylogenetic footprinting; discovering human gene function and predicting yeast gene function

Readership

Microbiologists and Yeast researchers (yeast molecular biologists, cell biologists) C.elegans/mouse/other eukaryote system researchers possibly seeking to utilise yeast as a simple model system

Table of Contents

  • Introduction to functional analysis in yeast
    Yeast genetics and strain construction
    Yeast transformation
    A guided tour to PCR-based genomic manipulations of S. cerevisiae (PCR-targeting)
    Studying essential genes:
    generating and using promoter fusions and conditional alleles
    Yeast hybrid approaches
    Array-based yeast two-hybrid screening for protein-protein interactions
    Reporter genes and their uses in studying yeast gene expression
    Transcript analysis: a microarray approach
    GFP-based microscopic approaches for whole chromosome analysis in yeasts
    Immunological methods
    Measuring the proximity of proteins in living cells by fluorescence resonance energy transfer between CFP and YFP
    Identification, characterization, and phenotypic analysis of covalently linked cell wall Proteins
    Yeast protein microarrays
    Smart genetic screens
    High-throughput strain construction and systematic synthetic lethal Screening in Saccharomyces cerevisiae
    Chemical genomic tools for understanding gene function and drug action
    RNA gene analysis
    Analysis of gene function of mitochondria
    Yeast prions and their analysis in vivo
    Metabolic control in the eukaryotic cell, a Systems Biology perspective
    Phylogenetic footprinting
    Saccharomyces cerevisiae as a tool for human gene function discovery
    Bioinformatic prediction of yeast gene function
    Yeast genetic strain and plasmid collections
    Yeast gene analysis: the future

Product details

  • No. of pages: 730
  • Language: English
  • Copyright: © Academic Press 2007
  • Published: May 25, 2007
  • Imprint: Academic Press
  • Hardcover ISBN: 9780123694782
  • eBook ISBN: 9780080521787

About the Serial Volume Editor

Ian Stansfield

Affiliations and Expertise

University of Aberdeen, Foresterhill, U.K.

About the Series Volume Editor

Mike Stark

Affiliations and Expertise

University of Dundee, U.K.

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