Focusing on Saccharomyces cerevisiae, the second edition of Yeast Gene Analysis represents a major reworking of the original edition, with many completely new chapters and major revisions to all previous chapters. Originally published shortly after completion of the yeast genome sequence, the new edition covers many of the major genome-wide strategies that have been developed since then such as microarray analysis of transcription, synthetic gene array studies, protein microarrays and chemical genetic approaches. It represents a valuable resource for any research laboratory using budding yeast as their experimental system in which to identify new yeast gene functions. The chapters are written in a readable style with useful background information, technical tips and specific experimental protocols included as appropriate, enabling both the novice and the experienced yeast researcher to adopt new procedures with confidence.

Key Features

New chapters on: * strain construction * genome-wide two-hybrid approaches * use of microarrays for transcript analysis * real-time analysis of chromosome behaviour and FRET * synthetic gene array technology and protein arrays * chemical genomics and yeast prions * RNA gene analysis and mitochondrial gene function analysis * phylogenetic footprinting * discovering human gene function and predicting yeast gene function


Microbiologists and Yeast researchers (yeast molecular biologists, cell biologists) C.elegans/mouse/other eukaryote system researchers possibly seeking to utilise yeast as a simple model system

Table of Contents

Preface Introduction to functional analysis in yeast Yeast genetics and strain construction Yeast transformation A guided tour to PCR-based genomic manipulations of S. cerevisiae (PCR-targeting) Studying essential genes: generating and using promoter fusions and conditional alleles Yeast hybrid approaches Array-based yeast two-hybrid screening for protein-protein interactions Reporter genes and their uses in studying yeast gene expression Transcript analysis: a microarray approach GFP-based microscopic approaches for whole chromosome analysis in yeasts Immunological methods Measuring the proximity of proteins in living cells by fluorescence resonance energy transfer between CFP and YFP Identification, characterization, and phenotypic analysis of covalently linked cell wall Proteins Yeast protein microarrays Smart genetic screens High-throughput strain construction and systematic synthetic lethal Screening in Saccharomyces cerevisiae Chemical genomic tools for understanding gene function and drug action RNA gene analysis Analysis of gene function of mitochondria Yeast prions and their analysis in vivo Metabolic control in the eukaryotic cell, a Systems Biology perspective Phylogenetic footprinting Saccharomyces cerevisiae as a tool for human gene function discovery Bioinformatic prediction of yeast gene function Yeast genetic strain and plasmid collections Yeast gene analysis: the future


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© 2007
Academic Press
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