Ultrasensitive and Rapid Enzyme Immunoassay - 1st Edition - ISBN: 9780444502025, 9780080858968

Ultrasensitive and Rapid Enzyme Immunoassay, Volume 27

1st Edition

Authors: E. Ishikawa
Paperback ISBN: 9780444502025
eBook ISBN: 9780080858968
Imprint: Elsevier Science
Published Date: 30th September 1999
Page Count: 333
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Table of Contents

Chapter 1. Preface. Introduction: classification of immunoassays.

  1. History of ultrasensitive enzyme immunoassay.
  2. Factors limiting the sensitivity of noncompetitive heterogeneous solid phase enzyme immunoasssays.
  3. Methods to minimize effects of factors limiting the sensitivity.
  4. Principle of the immune complex transfer enzyme immunoassay.
  5. Potential of the immune complex transfer enzyme immunoassay to improve the sensitivity and its limitations.
  6. Ultrasensitive and rapid enzyme immunoassay.
  7. Protocol of immune complex transfer enzyme immunoassay.
  8. Ultrasensitive immunoassay for haptens.
  9. Properties of the reagents for enzyme-labeling.
  10. Enzyme-labeling of antibodies.
  11. Preparation and affinity-purification of IgG fragments.
  12. Protocol for enzyme-labeling of antibodies.
  13. Protocol for labeling avidin with 2,4-dinitrophenyl groups and &bgr;-D-galactosidase.
  14. Enzyme-labeling of antigens.
  15. Protocol for enzyme-labeling of antigens.
  16. Assays of enzymes. References. Subject Index.

Description

Chapter 1. Preface. Introduction: classification of immunoassays.

  1. History of ultrasensitive enzyme immunoassay.
  2. Factors limiting the sensitivity of noncompetitive heterogeneous solid phase enzyme immunoasssays.
  3. Methods to minimize effects of factors limiting the sensitivity.
  4. Principle of the immune complex transfer enzyme immunoassay.
  5. Potential of the immune complex transfer enzyme immunoassay to improve the sensitivity and its limitations.
  6. Ultrasensitive and rapid enzyme immunoassay.
  7. Protocol of immune complex transfer enzyme immunoassay.
  8. Ultrasensitive immunoassay for haptens.
  9. Properties of the reagents for enzyme-labeling.
  10. Enzyme-labeling of antibodies.
  11. Preparation and affinity-purification of IgG fragments.
  12. Protocol for enzyme-labeling of antibodies.
  13. Protocol for labeling avidin with 2,4-dinitrophenyl groups and &bgr;-D-galactosidase.
  14. Enzyme-labeling of antigens.
  15. Protocol for enzyme-labeling of antigens.
  16. Assays of enzymes. References. Subject Index.

Details

No. of pages:
333
Language:
English
Copyright:
© Elsevier Science 1999
Published:
Imprint:
Elsevier Science
eBook ISBN:
9780080858968
Paperback ISBN:
9780444502025

About the Authors

E. Ishikawa Author

Affiliations and Expertise

Department of Biochemistry, Miyazaki Medical College, Miyazaki 889-1692, Japan