Two-Component Signaling Systems, Part C

Two-Component Signaling Systems, Part C

1st Edition - March 1, 2010

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  • Editors: Melvin Simon, Brian Crane, Alexandrine Crane
  • Hardcover ISBN: 9780123813473
  • eBook ISBN: 9780123813480

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Multicellular organisms must be able to adapt to cellular events to accommodate prevailing conditions. Sensory-response circuits operate by making use of a phosphorylation control mechanism known as the "two-component system." This volume, the third in a three-volume treatment edited by the same group of editors, includes a wide range of methods, including those dealing with the Sln-1 kinase pathway, triazole sensitivity in C. albicans, and histidine kinases in cyanobacteria circadian clock.

Key Features

  • Includes time-tested core methods and new innovations applicable to any researcher studing two-component signaling systems or histidine kinases
  • Methods included are useful to both established researchers and newcomers to the field
  • Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines


Researchers and students in biochemistry, genetics, and molecular biology

Table of Contents

    1. Characterizing cross-talk in vivo: avoiding pitfalls and over-interpretation
      Albert Siryaporn and Mark Goulian
    2. Inference of Direct Residue Contacts in Two-Component Signaling
      Bryan Lunt, Hendrik Szurmant, Andrea Procaccini, James A. Hoch, Terence Hwa and Martin Weigt
    3. Computational Modeling of Phosphotransfer Complexes in Two-Component Signaling
      Alexander Schug, Martin Weigt, James A. Hoch, Jose N. Onuchic, Terence Hwa, Hendrik Szurmant
    4. Kinetic studies of the yeast His-Asp phosphorelay signaling pathway
      Alla O. Kaserer, Babak Andi, Paul F. Cook and Ann H. West
    5. Purification of MBP-EnvZ fusion proteins using an automated system
      Ricardo Oropeza and Edmundo Calva
    6. Measurement of Response Regulator Autodephosphorylation Rates Spanning Six Orders of Magnitude
      Robert B. Bourret, Stephanie A. Thomas, Stephani C. Page, Rachel L. Creager-Allen, Aaron M. Moore, and Ruth E. Silversmith
    7. Transmembrane receptors chimeras to probe Hamp domain function
      Jürgen U. Linder and Joachim E. Schultz
    8. Light-Activated Bacterial LOV-domain Histidine Kinases
      Tong-Seung Tseng, Marcus A. Frederickson, Winslow R. Briggs and Roberto A. Bogomolni
    9. Characterization of Bacteriophytochromes from Photosynthetic Bacteria: Histidine Kinase Signaling Triggered by light and redox sensing
      Eric Giraud, Jérôme Lavergne and André Verméglio
    10. Biophysical assays for protein interactions in the Wsp sensory system and biofilm formation
      Nabanita De, Marcos V.A.S. Navarro, Qi Wang, Petya V. Krasteva and Holger Sondermann
    11. High throughput screening of bacterial protein localization
      John N. Werner and Zemer Gitai
    12. In vitro and in vivo analysis of the ArcB/A redox signaling pathway
      Adrián F. Alvarez and Dimitris Georgellis
    13. Potassium sensing histidine kinase in Bacillus subtilis
      Daniel López, Erin Gontang and Roberto Kolter
    14. Two Component Systems and Regulation of Developmental Progression in Myxococcus Xanthus
      Bongsoo Lee, Andreas Schramm, and Penelope I. Higgs
    15. Two-component signaling to the stress MAP kinase cascade in fission yeast
      Susumu Morigasaki and Kazuhiro Shiozaki
    16. Genetic and Biochemical Analysis of the SLN1 Pathway in Saccharomyces cerevisiae
      Jan S. Fassler
    17. Analysis of mitogen-activated protein kinase phosphorylation in response to stimulation of histidine kinase signaling pathways in Neurospora
      Carol A. Jones and Katherine A. Borkovich
    18. Biochemical Characterization of Plant Hormone Cytokinin Receptor Histidine Kinases Using Microorganisms
      Takeshi Mizuno and Takafumi Yamashino
    19. Characterization of Pseudo-Response Regulators In Plants
      Woe-Yeon Kim, Patrice A. Salomé, Sumire Fujiwara, David E. Somers and C. Robertson McClung
    20. Reversible Histidine Phosphorylation in Mammalian Cells: A Teeter-Totter Formed by Nucleoside Diphosphate Kinase and Protein Histidine Phosphatase
      Thomas Wieland, Hans-Jörg Hippe, Katrin Ludwig, Xiao-Bo Zhou, Michael Korth and Susanne Klumpp
    21. Histidine phosphorylation in histones and in other mammalian proteins
      Paul G. Besant and P.V. Attwood



Product details

  • No. of pages: 496
  • Language: English
  • Copyright: © Academic Press 2010
  • Published: March 1, 2010
  • Imprint: Academic Press
  • Hardcover ISBN: 9780123813473
  • eBook ISBN: 9780123813480

About the Serial Volume Editors

Melvin Simon

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA

Brian Crane

Affiliations and Expertise

Cornell University, Ithaca, NY, USA

Alexandrine Crane

Affiliations and Expertise

Cornell University, Ithaca, NY, USA

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