Description

Multicellular organisms must be able to adapt to cellular events to accommodate prevailing conditions. Sensory-response circuits operate by making use of a phosphorylation control mechanism known as the "two-component system." This volume, the third in a three-volume treatment edited by the same group of editors, includes a wide range of methods, including those dealing with the Sln-1 kinase pathway, triazole sensitivity in C. albicans, and histidine kinases in cyanobacteria circadian clock.

Key Features

* Includes time-tested core methods and new innovations applicable to any researcher studing two-component signaling systems or histidine kinases
* Methods included are useful to both established researchers and newcomers to the field
* Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines

Readership

Researchers and students in biochemistry, genetics, and molecular biology

Table of Contents

  1. Characterizing cross-talk in vivo: avoiding pitfalls and over-interpretation
    Albert Siryaporn and Mark Goulian
  2. Inference of Direct Residue Contacts in Two-Component Signaling
    Bryan Lunt, Hendrik Szurmant, Andrea Procaccini, James A. Hoch, Terence Hwa and Martin Weigt
  3. Computational Modeling of Phosphotransfer Complexes in Two-Component Signaling
    Alexander Schug, Martin Weigt, James A. Hoch, Jose N. Onuchic, Terence Hwa, Hendrik Szurmant
  4. Kinetic studies of the yeast His-Asp phosphorelay signaling pathway
    Alla O. Kaserer, Babak Andi, Paul F. Cook and Ann H. West
  5. Purification of MBP-EnvZ fusion proteins using an automated system
    Ricardo Oropeza and Edmundo Calva
  6. Measurement of Response Regulator Autodephosphorylation Rates Spanning Six Orders of Magnitude
    Robert B. Bourret, Stephanie A. Thomas, Stephani C. Page, Rachel L. Creager-Allen, Aaron M. Moore, and Ruth E. Silversmith
  7. Transmembrane receptors chimeras to probe Hamp domain function
    Jürgen U. Linder and Joachim E. Schultz
  8. Light-Activated Bacterial LOV-domain Histidine Kinases
    Tong-Seung Tseng, Marcus A. Frederickson, Winslow R. Briggs and Roberto A. Bogomolni
  9. Characterization of Bacteriophytochromes from Photosynthetic Bacteria: Histidine Kinase Signaling Triggered by light and redox sensing
    Eric Giraud, Jérôme Lavergne and André Verméglio
  10. Biophysical assays for protein interactions in the Wsp sensory system and biofilm formation
    Nabanita De, Marcos V.A.S. Navarro, Qi Wang, Petya V. Krasteva and Holger Sondermann
  11. High throughput screening of bacterial protein localization
    John N. Werner and Zemer Gitai
  12. In vitro and in vivo an

Details

No. of pages:
496
Language:
English
Copyright:
© 2010
Published:
Imprint:
Academic Press
Electronic ISBN:
9780123813480
Print ISBN:
9780123813473

About the serial-volume-editors