Multicellular organisms must be able to adapt to cellular events to accommodate prevailing conditions. Sensory-response circuits operate by making use of a phosphorylation control mechanism known as the "two-component system." Sections include: Computational Analyses of Sequences and Sequence Alignments Biochemical and Genetic Assays of Individual Components of Signaling Systems Physiological Assays and Readouts

Key Features

* Presents detailed protocols * Includes troubleshooting tips


Biochemists and researchers in related life science fields.

Table of Contents

Section I. Computational Analyses of Sequences and Sequence Alignments CHAPTER 1: Comparative genomic and protein sequence analyses of a complex system controlling bacterial chemotaxis. CHAPTER 2: Two component systems in microbial communities: Approaches and resources for analyzing metagenomic data sets. CHAPTER 3: Identification of sensory and signal-transducing domains in two-component signaling systems. CHAPTER 4: Features of protein-protein interactions in two-component signaling deduced from genomic libraries. CHAPTER 5: Sporulation phosphorelay proteins and their complexes: Crystallographic characterization. CHAPTER 6: Control Analysis of Bacterial Chemotaxis Signaling. CHAPTER 7: Classification of Response Regulators based on their Surface Properties Section II. Biochemical and Genetic Assays of Individual Components of the Signaling Systems CHAPTER 8: Purification and Assays of Rhodobacter capsulatus RegB-RegA Two Component Signal Transduction System. CHAPTER 9: Purification and reconstitution of PYP-phytochrome (Ppr) with biliverdin and 4-hydroxycinnamic acid. CHAPTER 10: Oxygen and Redox-Sensing by Two-Component Systems That Regulate Behavioral Responses. Behavioral Assays and Structural Studies of Aer using in vivo Disulfide Crosslinking. CHAPTER 11: Two-Component Signaling in the Virulence of S. aureus: A Silkworm Larvae-Pathogenic Agent Infection Model of Virulence. CHAPTER 12: TonB System, In vivo Assays and Characterization CHAPTER 13: Biochemical Characterization of Plant Ethylene Receptors following Transgenic Expression in Yeast. CHAPTER 14: Structure of SixA, a histidine protein phosphatase of the ArcB HPt domain in E. coli. CHAPTER 15: Triggering and monitoring light-sensing reactions in protein cr


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© 2007
Academic Press
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