Two-Component Signaling Systems, Part A, Volume 422

Section I. Computational Analyses of Sequences and Sequence Alignments

CHAPTER 1: Comparative genomic and protein sequence analyses of a complex system controlling bacterial chemotaxis. CHAPTER 2: Two component systems in microbial communities: Approaches and resources for analyzing metagenomic data sets. CHAPTER 3: Identification of sensory and signal-transducing domains in two-component signaling systems. CHAPTER 4: Features of protein-protein interactions in two-component signaling deduced from genomic libraries. CHAPTER 5: Sporulation phosphorelay proteins and their complexes: Crystallographic characterization. CHAPTER 6: Control Analysis of Bacterial Chemotaxis Signaling. CHAPTER 7: Classification of Response Regulators based on their Surface Properties

Section II. Biochemical and Genetic Assays of Individual Components of the Signaling Systems

CHAPTER 8: Purification and Assays of Rhodobacter capsulatus RegB-RegA Two Component Signal Transduction System. CHAPTER 9: Purification and reconstitution of PYP-phytochrome (Ppr) with biliverdin and 4-hydroxycinnamic acid. CHAPTER 10: Oxygen and Redox-Sensing by Two-Component Systems That Regulate Behavioral Responses. Behavioral Assays and Structural Studies of Aer using in vivo Disulfide Crosslinking. CHAPTER 11: Two-Component Signaling in the Virulence of S. aureus: A Silkworm Larvae-Pathogenic Agent Infection Model of Virulence. CHAPTER 12: TonB System, In vivo Assays and Characterization CHAPTER 13: Biochemical Characterization of Plant Ethylene Receptors following Transgenic Expression in Yeast. CHAPTER 14: Structure of SixA, a histidine protein phosphatase of the ArcB HPt domain in E. coli. CHAPTER 15: Triggering and monitoring light-sensing reactions in protein crystals CHAPTER 16 CHAPTER 17: Application of Fluorescence Resonance Energy Transfer to Examine EnvZ/OmpR Interactions CHAPTER 18: Gene promoter scan (GPS) methodology for identifying and classifying co-regulated promoters. CHAPTER 19: Targeting two-component signal transduction: A novel drug discovery system. CHAPTER 20: The essential YycFG two–component system of Bacillus subtilis

Section III. Physiological Assays and Readouts

CHAPTER 21: Isolation and Characterization of Chemotaxis Mutants of the Lyme Disease Spirochete Borrelia burgdorferi Using Allelic Exchange Mutagenesis, Flow Cytometry and Cell Tracking. CHAPTER 22: Phosphorylation assays of chemotaxis two-component system proteins in Borrelia burgdorferi. CHAPTER 23: Regulation of Respiratory Genes by ResD-ResE Signal Transduction System in Bacillus subtilis. CHAPTER 24: Physiological and genetic characterization of two-component systems in Myxococcus. CHAPTER 25: Detection and measurement of two-component systems that control dimorphism and virulence in fungi. CHAPTER 26: Using Two-Component Systems and other Bacterial Regulatory Factors for the Fabrication of Synthetic Genetic Devices.

Multicellular organisms must be able to adapt to cellular events to accommodate prevailing conditions. Sensory-response circuits operate by making use of a phosphorylation control mechanism known as the "two-component system."

Sections include: Computational Analyses of Sequences and Sequence Alignments Biochemical and Genetic Assays of Individual Components of Signaling Systems Physiological Assays and Readouts

• Presents detailed protocols
• Includes troubleshooting tips

Biochemists and researchers in related life science fields.