For over fifty years the Methods in Enzymology series has been the critically aclaimed laboratory standard and one of the most respected publications in the field of biochemistry. The highly relevant material makes it an essential publication for researchers in all fields of life and related sciences. This volume, the first of three on the topic of Translation Initiation includes articles written by leaders in the field.


Biochemists and researchers in related life science disciplines.

Table of Contents

Chapter 1 Use of Reticulocyte Lysates for Mechanistic Studies of Eukaryotic Translation Initiation William C. Merrick and Diane Barth-Baus Chapter 2 Studying translational control in Drosophila cell-free systems Fátima Gebauer and Matthias W. Hentze Chapter 3 Use of In Vitro Translation Extract Depleted in Specific Initiation Factors for the Investigation of Translational Regulation Daniel R. Gallie Chapter 4 Viral translation and replication in vitro Yuri V. Svitkin and Nahum Sonenberg Chapter 5 A practical approach to isolate 48S complexes: Affinity purification and analyses Nicolas Locker & Peter J. Lukavsky Chapter 6 Yeast phenotypic assays on translational control Bumjun Lee, Tsuyoshi Udagawa, Chingakham Ranjit Singh, and Katsura Asano Chapter 7 Localization and characterization of protein-protein interaction sites Chingakham Ranjit Singh and Katsura Asano* Chapter 8 In vivo stabilization of pre-initiation complexes by formaldehyde cross-linking. Leos Valášek, Bela Szamecz, Alan G. Hinnebusch and Klaus H. Nielsen Chapter 9 Molecular Genetic Structure-Function Analysis of Translation Initiation Factor eIF5B Byung-Sik Shin and Thomas E Dever Chapter 10 The use of fungal in vitro systems for studying translational regulation Cheng Wu, Nadia Amrani, Allan Jacobson, and Matthew S. Sachs Chapter 11 Investigating translation initiation using Drosophila molecular genetics Gritta Tettweiler and Paul Lasko


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© 2007
Academic Press
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