Translation Initiation: Extract Systems and Molecular Genetics

Translation Initiation: Extract Systems and Molecular Genetics

1st Edition - October 15, 2007
This is the Latest Edition
  • Editor: Jon Lorsch
  • Hardcover ISBN: 9780123741912
  • eBook ISBN: 9780080889665

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Description

For over fifty years the Methods in Enzymology series has been the critically aclaimed laboratory standard and one of the most respected publications in the field of biochemistry. The highly relevant material makes it an essential publication for researchers in all fields of life and related sciences. This volume, the first of three on the topic of Translation Initiation includes articles written by leaders in the field.

Readership

Biochemists and researchers in related life science disciplines.

Table of Contents

  • Chapter 1
    Use of Reticulocyte Lysates for Mechanistic Studies of Eukaryotic Translation Initiation
    William C. Merrick and Diane Barth-Baus

    Chapter 2
    Studying translational control in Drosophila cell-free systems
    Fátima Gebauer and Matthias W. Hentze

    Chapter 3
    Use of In Vitro Translation Extract Depleted in Specific Initiation Factors for the Investigation of Translational Regulation
    Daniel R. Gallie

    Chapter 4
    Viral translation and replication in vitro
    Yuri V. Svitkin and Nahum Sonenberg

    Chapter 5
    A practical approach to isolate 48S complexes: Affinity purification and analyses
    Nicolas Locker & Peter J. Lukavsky

    Chapter 6
    Yeast phenotypic assays on translational control
    Bumjun Lee, Tsuyoshi Udagawa, Chingakham Ranjit Singh, and Katsura Asano

    Chapter 7
    Localization and characterization of protein-protein interaction sites
    Chingakham Ranjit Singh and Katsura Asano*

    Chapter 8
    In vivo stabilization of pre-initiation complexes by formaldehyde cross-linking.
    Leos Valášek, Bela Szamecz, Alan G. Hinnebusch and Klaus H. Nielsen

    Chapter 9
    Molecular Genetic Structure-Function Analysis of Translation Initiation Factor eIF5B
    Byung-Sik Shin and Thomas E Dever

    Chapter 10
    The use of fungal in vitro systems for studying translational regulation
    Cheng Wu, Nadia Amrani, Allan Jacobson, and Matthew S. Sachs

    Chapter 11
    Investigating translation initiation using Drosophila molecular genetics
    Gritta Tettweiler and Paul Lasko

    Chapter 12
    Analysis of RNA:protein interactions in vivo: Identification of RNA binding partners of nuclear factor 90
    Andrew M. Parrott, Melissa R. Walsh and Michael B. Mathews

    Chapter 13
    Approaches for Analyzing the Differential Activities and Functions of eIF4E Family Members
    Robert E. Rhoads, Tzvetanka D. Dinkova, and Rosemary Jagus

    Chapter 14
    Tethered function assays: An adaptable approach to study RNA regulatory proteins
    Jeff Coller. Mary Wickens

    Chapter 15
    Analysis of ribosomal shunting during translation initiation in eukaryotic mRNAs
    Vincent P. Mauro, Stephen A. Chappell, and John Dresios

Product details

  • No. of pages: 424
  • Language: English
  • Copyright: © Academic Press 2007
  • Published: October 15, 2007
  • Imprint: Academic Press
  • Hardcover ISBN: 9780123741912
  • eBook ISBN: 9780080889665

About the Serial Volume Editor

Jon Lorsch