Tissue Culture Technique, Second Edition, provides an introduction to tissue culture techniques. An attempt has been made to reduce all equipment and procedure to their simplest forms without omitting steps necessary to ensure successful cultures. Sufficient detail is given to enable acquisition of the essentials of the techniques and avoidance of the many pitfalls which may be encountered by beginners, and may sometimes beset those more experienced.
The first few chapters of this book are devoted to the choice and organization of the laboratory rooms and their equipment, including glassware, instruments, etc. Attention is also given to methods of preparation of supplies for use in the various techniques. The succeeding chapters describe the preparation of the culture media and the tissues, as well as the preparation of the cultures in various ways. Also discussed are the types of cells one may expect to see growing from a given tissue, methods of recording their behavior and measuring their growth, as well as their significance in the interpretation of experimental results. The last chapters treat methods of applying micrurgical, histological, and photomicrographic techniques to tissue cultures.
It is hoped that the carefully considered data presented in this book, and the many details which are the result of long experience, may be of real service to the prospective worker.
Foreword to the Second Edition Author's Preface Chapter I. Introduction to Tissue Culture Chapter II. Basic Considerations of Space and Equipment Cleanliness, Ventilation, Lighting One Room, Cubicle. Two or More Rooms Permanent Equipment and Its Care Chapter III. Details of Equipment Glassware, Instruments, Accessories Amounts Required Cleansing and Packing Chapter IV. Sterilizing Procedures General Precautions Use of Heat: Dry Heat, Autoclave, Arnold Filtration Chapter V. Physiological Solutions Preparation of Tyrode Solution, Hydrogen Ion Concentration, Osmotic Pressure Methods of Sterilization: Autoclave, Filtration Storage Sterility Tests Formula for Various Physiological Solutions Chapter VI. Plasma and Serum Preparation of Paraffined Tubes Obtaining Plasma of Chickens: From Carotid Artery, Wing, Heart—Desiccated Plasma Use of Anticoagulants Obtaining Blood of Mammals Obtaining Blood of Cold-Blooded Animals Preparation of Serum From Plasma Chapter VII. Preparation of Tissue Extracts Preparation of Small Amounts: Chick, Mammalian and Amphibian Embryonic Extracts Preparation of Large Amounts; Use of Mechanical Devices Desiccated Extracts Adult Tissue Extracts—Storage; Freezing Chapter VIII. Artificial Media Media of Known Composition Fibrinogen and Thrombin Chapter IX. Preparation of Tissues Warm Blooded: Embryonic, Adult—Normal and Malignant Cold Blooded: Vertebrate, Invertebrate Storage of Tissues Obtaining Pure Cell Cultures Chapter X. Choice of Methods for Culturing Chapter XI. Cultures on Coverslips Preparation on Single and Double Coverslips, Renewal of Medium, Transfer For Growth, Cytological Study Chapter XII. Cultures in Flasks Preparation in Carrel or Erlenmeyer, Use of Perforated Cellophane, Use of Fibrinogen Renewal of Medium, Transfer
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- © Academic Press 1950
- 1st January 1950
- Academic Press
- eBook ISBN: