Tissue Culture Technique

Tissue Culture Technique

2nd Edition - January 1, 1950

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  • Author: Gladys Cameron
  • eBook ISBN: 9781483269030

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Tissue Culture Technique, Second Edition, provides an introduction to tissue culture techniques. An attempt has been made to reduce all equipment and procedure to their simplest forms without omitting steps necessary to ensure successful cultures. Sufficient detail is given to enable acquisition of the essentials of the techniques and avoidance of the many pitfalls which may be encountered by beginners, and may sometimes beset those more experienced. The first few chapters of this book are devoted to the choice and organization of the laboratory rooms and their equipment, including glassware, instruments, etc. Attention is also given to methods of preparation of supplies for use in the various techniques. The succeeding chapters describe the preparation of the culture media and the tissues, as well as the preparation of the cultures in various ways. Also discussed are the types of cells one may expect to see growing from a given tissue, methods of recording their behavior and measuring their growth, as well as their significance in the interpretation of experimental results. The last chapters treat methods of applying micrurgical, histological, and photomicrographic techniques to tissue cultures. It is hoped that the carefully considered data presented in this book, and the many details which are the result of long experience, may be of real service to the prospective worker.

Table of Contents

  • Foreword to the Second Edition

    Author's Preface

    Chapter I. Introduction to Tissue Culture

    Chapter II. Basic Considerations of Space and Equipment

    Cleanliness, Ventilation, Lighting

    One Room, Cubicle. Two or More Rooms

    Permanent Equipment and Its Care

    Chapter III. Details of Equipment

    Glassware, Instruments, Accessories

    Amounts Required

    Cleansing and Packing

    Chapter IV. Sterilizing Procedures

    General Precautions

    Use of Heat: Dry Heat, Autoclave, Arnold


    Chapter V. Physiological Solutions

    Preparation of Tyrode Solution, Hydrogen Ion Concentration, Osmotic Pressure

    Methods of Sterilization: Autoclave, Filtration


    Sterility Tests

    Formula for Various Physiological Solutions

    Chapter VI. Plasma and Serum

    Preparation of Paraffined Tubes

    Obtaining Plasma of Chickens: From Carotid Artery, Wing, Heart—Desiccated Plasma

    Use of Anticoagulants

    Obtaining Blood of Mammals

    Obtaining Blood of Cold-Blooded Animals

    Preparation of Serum From Plasma

    Chapter VII. Preparation of Tissue Extracts

    Preparation of Small Amounts: Chick, Mammalian and Amphibian Embryonic Extracts

    Preparation of Large Amounts; Use of Mechanical Devices

    Desiccated Extracts

    Adult Tissue Extracts—Storage; Freezing

    Chapter VIII. Artificial Media

    Media of Known Composition

    Fibrinogen and Thrombin

    Chapter IX. Preparation of Tissues

    Warm Blooded: Embryonic, Adult—Normal and Malignant

    Cold Blooded: Vertebrate, Invertebrate

    Storage of Tissues

    Obtaining Pure Cell Cultures

    Chapter X. Choice of Methods for Culturing

    Chapter XI. Cultures on Coverslips

    Preparation on Single and Double Coverslips, Renewal of Medium, Transfer

    For Growth, Cytological Study

    Chapter XII. Cultures in Flasks

    Preparation in Carrel or Erlenmeyer, Use of Perforated Cellophane, Use of Fibrinogen

    Renewal of Medium, Transfer

    For Encouragement of Differentiation, Study of Metabolic Products of the Cells, Propagation of Viruses

    Chapter XIII. Cultures in Roller Tubes

    Preparation, Renewal of Medium, Transfer

    For Study of Metabolic Products and Nutritional Requirements of Cells, Maintenance of Strains of Cells

    Chapter XIV. What One Sees in Tissue Cultures

    Explants and Outgrowths

    Types of Cells in Outgrowths from Various Tissues

    Dark Field Illumination and Phase Contrast Microscopy

    Chapter XV. Some Experimental Procedures

    Need for Exactitude in Preparation and Selection of Cultures, Control and Experimental

    Keeping of Records, Cytological and Physiological Studies, Growth Measurements

    Chapter XVI. Micromanipulation of Tissue Cultures

    Chapter XVII. Histological Techniques

    Vital Staining

    Fixation and Staining


    Chapter XVIII. Photomicrography of Tissue Cultures


    A. List of Precautions to Insure Good Growth

    B. Conversion Tables

    C. Sources of Supply for Special Tissue Culture Materials


    Supplementary References


Product details

  • No. of pages: 204
  • Language: English
  • Copyright: © Academic Press 1950
  • Published: January 1, 1950
  • Imprint: Academic Press
  • eBook ISBN: 9781483269030

About the Author

Gladys Cameron

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