Tissue Culture Technique - 2nd Edition - ISBN: 9781483233154, 9781483269030

Tissue Culture Technique

2nd Edition

Authors: Gladys Cameron
eBook ISBN: 9781483269030
Imprint: Academic Press
Published Date: 1st January 1950
Page Count: 204
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Tissue Culture Technique, Second Edition, provides an introduction to tissue culture techniques. An attempt has been made to reduce all equipment and procedure to their simplest forms without omitting steps necessary to ensure successful cultures. Sufficient detail is given to enable acquisition of the essentials of the techniques and avoidance of the many pitfalls which may be encountered by beginners, and may sometimes beset those more experienced. The first few chapters of this book are devoted to the choice and organization of the laboratory rooms and their equipment, including glassware, instruments, etc. Attention is also given to methods of preparation of supplies for use in the various techniques. The succeeding chapters describe the preparation of the culture media and the tissues, as well as the preparation of the cultures in various ways. Also discussed are the types of cells one may expect to see growing from a given tissue, methods of recording their behavior and measuring their growth, as well as their significance in the interpretation of experimental results. The last chapters treat methods of applying micrurgical, histological, and photomicrographic techniques to tissue cultures.
It is hoped that the carefully considered data presented in this book, and the many details which are the result of long experience, may be of real service to the prospective worker.

Table of Contents

Foreword to the Second Edition

Author's Preface

Chapter I. Introduction to Tissue Culture

Chapter II. Basic Considerations of Space and Equipment

Cleanliness, Ventilation, Lighting

One Room, Cubicle. Two or More Rooms

Permanent Equipment and Its Care

Chapter III. Details of Equipment

Glassware, Instruments, Accessories

Amounts Required

Cleansing and Packing

Chapter IV. Sterilizing Procedures

General Precautions

Use of Heat: Dry Heat, Autoclave, Arnold


Chapter V. Physiological Solutions

Preparation of Tyrode Solution, Hydrogen Ion Concentration, Osmotic Pressure

Methods of Sterilization: Autoclave, Filtration


Sterility Tests

Formula for Various Physiological Solutions

Chapter VI. Plasma and Serum

Preparation of Paraffined Tubes

Obtaining Plasma of Chickens: From Carotid Artery, Wing, Heart—Desiccated Plasma

Use of Anticoagulants

Obtaining Blood of Mammals

Obtaining Blood of Cold-Blooded Animals

Preparation of Serum From Plasma

Chapter VII. Preparation of Tissue Extracts

Preparation of Small Amounts: Chick, Mammalian and Amphibian Embryonic Extracts

Preparation of Large Amounts; Use of Mechanical Devices

Desiccated Extracts

Adult Tissue Extracts—Storage; Freezing

Chapter VIII. Artificial Media

Media of Known Composition

Fibrinogen and Thrombin

Chapter IX. Preparation of Tissues

Warm Blooded: Embryonic, Adult—Normal and Malignant

Cold Blooded: Vertebrate, Invertebrate

Storage of Tissues

Obtaining Pure Cell Cultures

Chapter X. Choice of Methods for Culturing

Chapter XI. Cultures on Coverslips

Preparation on Single and Double Coverslips, Renewal of Medium, Transfer

For Growth, Cytological Study

Chapter XII. Cultures in Flasks

Preparation in Carrel or Erlenmeyer, Use of Perforated Cellophane, Use of Fibrinogen

Renewal of Medium, Transfer

For Encouragement of Differentiation, Study of Metabolic Products of the Cells, Propagation of Viruses

Chapter XIII. Cultures in Roller Tubes

Preparation, Renewal of Medium, Transfer

For Study of Metabolic Products and Nutritional Requirements of Cells, Maintenance of Strains of Cells

Chapter XIV. What One Sees in Tissue Cultures

Explants and Outgrowths

Types of Cells in Outgrowths from Various Tissues

Dark Field Illumination and Phase Contrast Microscopy

Chapter XV. Some Experimental Procedures

Need for Exactitude in Preparation and Selection of Cultures, Control and Experimental

Keeping of Records, Cytological and Physiological Studies, Growth Measurements

Chapter XVI. Micromanipulation of Tissue Cultures

Chapter XVII. Histological Techniques

Vital Staining

Fixation and Staining


Chapter XVIII. Photomicrography of Tissue Cultures


A. List of Precautions to Insure Good Growth

B. Conversion Tables

C. Sources of Supply for Special Tissue Culture Materials


Supplementary References



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© Academic Press 1950
Academic Press
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About the Author

Gladys Cameron

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