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Three Phase Partitioning - 1st Edition - ISBN: 9780128244180

Three Phase Partitioning

1st Edition

Applications in Separation and Purification of Biological Molecules and Natural Products

Editors: Munishwar Gupta Ipsita Roy
Paperback ISBN: 9780128244180
Imprint: Elsevier
Published Date: 1st July 2021
Page Count: 276
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Three Phase Partitioning: Applications in Separation and Purification of Biological Molecules and Natural Products presents applications in diverse areas of both chemical technology and biotechnology. This book serves as a single resource for learning about both the economical, facile and scalable processes, along with their potential for applications in the separation and purification of materials and compounds across the entire spectra of chemical and biological nature. The book begins by explaining the origins and fundamentals of TPP and continues with chapters on related applications, ranging from the purification of parasite recombinant proteases to oil extraction from oilseeds and oleaginous microbes, and more.

Key Features

  • Written by researchers who have been pioneers in developing and utilizing three phase partitioning
  • Focuses on applications, with chapters detailing relevance to a wide variety of areas and numerous practical examples
  • Designed to give laboratory workers the information needed to undertake the challenge of designing successful three-phase partitioning protocols


Research scholars and scientists working in various fields of recovery, separation and purification of proteins/enzymes, oils, carbohydrates and small molecular weight bioactive organic compounds in both academia and industrial sectors. Researchers working in extraction, separation and purification of natural products and biomolecules; industries looking to produce hyperactive enzymes for various applications

Table of Contents

  1. The origins of Three Phase Partitioning
    2. Three Phase Partitioning: How it got applied to areas other than protein concentration and purification
    3. The multiple facets of three phase partitioning in the purification, concentration and preservation of proteins
    4. Enzymes recovery by Three Phase Partitioning: an overview
    5. Emulsion gel formation in three phase partitioning
    6. Using three phase partitioning to purify parasite recombinant proteases
    7. Partitioning of plant peroxidases by Three Phase Partitioning
    8. Process optimization and purification of naringinase and asparaginase enzymes using three phase partitioning (TPP)
    9. Macro-[affinity ligand] facilitated three phase partitioning [MLFTPP]. Converting Three Phase Partitioning into an affinity-based process
    10. Applications of Three Phase Partitioning and Macro-[affinity ligand] facilitated three phase partitioning (MLFTPP) in protein refolding
    11. Three phase partitioning-based strategies for highly efficient separation of bioactive polysaccharides from natural resources
    12. Using Three Phase Partitioning to isolate carbohydrates of different molecular weights
    13. Technologies for oil extraction from oilseeds and oleaginous microbes: A comprehensive review
    14. Three Phase Partitioning as an extraction technique for oleageneous materials
    15. Three phase partitioning as a method for isolation of bioactive compounds from plant and animal tissues
    16. Three-phase partitioning for simultaneous extraction and purification of biomolecules


No. of pages:
© Elsevier 2021
1st July 2021
Paperback ISBN:

About the Editors

Munishwar Gupta

Dr. Munishwar Nath Gupta earned his PhD from Indian Institute of Science, Bengaluru, and completed post-doctoral positions at Massachusetts Institute of Technology (USA), University of Minnesota (USA), Lund University (Sweden), and University of Technology of Compiegne (France). He has taught chemistry, biochemistry, and biotechnology at Indian Institute of Technology, Delhi, between 1975-2016. He was awarded the National Science Talent fellowship (India) and Fellowships of National Academy of Sciences and Indian National Science Academy. He has edited three books on thermostability of enzymes, non-aqueous enzymology and affinity-based separation methods (published by Springer/Birkhauser). He was an Associate Editor of Biocatalysis and Biotransformation (Taylor and Francis) and founding and former editor-in-chief of Sustainable Chemical Processes (Springer). He’s served on editorial boards of several national and international journals and acted as a consultant to Novozyme (Denmark), Dabur (India), and other international companies. His research interests include applied biocatalysis and interfaces of biochemistry with nanotechnology.

Affiliations and Expertise

Formerly post-doctoral positions: Massachusetts Institute of Technology, USA, University of Minnesota, USA, Lund University, Sweden, University of Technology of Compiegne, France

Ipsita Roy

Dr. Ipsita Roy earned her PhD from Indian Institute of Technology, Delhi, completed a post-doctoral position at Indian Institute of Technology, Delhi, and conducted an Alexander von Humboldt research fellowship at the University of Bonn (Germany). She has been teaching biotechnology and biochemistry at the postgraduate level at National Institute of Pharmaceutical Education and Research, S.A.S. Nagar, since 2005. Her research interests include stabilization of pharmaceutically important and disease-relevant proteins; aptamer technology; downstream processing of proteins.

Affiliations and Expertise

Department of Biotechnology, National Institute of Pharmaceutical Education and Research (NIPER), India

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