Three-Dimensional Confocal Microscopy: Volume Investigation of Biological Specimens

1st Edition

Volume Investigation of Biological Specimens

Series Volume Editors: John Stevens Linda Mills Judy Trogadis
Hardcover ISBN: 9780126683301
eBook ISBN: 9780323138963
Imprint: Academic Press
Published Date: 1st September 1994
Page Count: 507
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Table of Contents

Preface. Confocal Microscopy: Practical Considerations: Introduction to Confocal Three-Dimensional Volume Investigation. Background Rejection and Optimization of Signal to Noise in Confocal Microscopy. Sources of Noise in Three-Dimensional Microscopical Data Sets. Simultaneous Ultraviolet and Visible Wavelength Confocal Microscopy. Fluorescent Labels for Confocal Microscopy. Display Methods for Gray-Scale, Voxel-Based Data Sets. Three-Dimensional Volume Reconstitution in Confocal Microscopy: Practical Considerations. Biological Applications of Confocal Microscopy: Imagine Ion Channels in Live Central Neurons Using Fluorescent Ligands. Imaging Ion Channels in Live Central Neurons Using Fluorescent Ligands II. Labeling of Cells and Tissues. Expression of G Protein-Coupled Receptors n Baculovirus/Sf9 Cells: Imaging Receptor Distribution by Confocal Fluorescence Microscopy. Dynamic Intracellular Calcium Compartments: Confocal Microscopy Using Fluo-3 in Cells and Organelles. Confocal Microscopy of Living Eggs and Embryos. Spatial Organization of Microtubules in PC12 Cells: Three-Dimensional Electron Microscopy and Confocal Microscopy Applied to Volume Investigation. Confocal Imaging of Living Neurons and Organelles. Confocal Microscopy in Diagnostic Cytology. Confocal Microscopy in Diagnostic Pathology. Alternative Approaches and Future Applications: Ultrathin Optical Sectioning and Dynamic Volume Investigation with Conventional Light Microscopy. Serial Electron Microscopy as an Alternative or Complement to Confocal Microscopy for the Study of Synapses and Dendritic Spines in the Central Nervous System. Confocal Scanning Opthal

Description

The integration of confocal microscopy and volume investigation has led to an unprecedented ability to examine spatial relationships between cellular structure and function. The goal of this book is to familiarize the reader with these new technologies and to demonstrate their applicability to a wide range of biological and clinical problems.

Key Features

@introbul:Key Features @bul:* Volume investigation

  • Three-dimensional reconstruction
  • Fluroescent probe design
  • Biological applications of confocal microscopy, including calcium imaging, receptor movement, and diagnostic pathology
  • Confocal data display and analysis
  • Twenty-eight pages of color

Readership

AUDIENCE: Cell biologists, molecular biologists, neurobiologists, and biomedical researchers.


Details

No. of pages:
507
Language:
English
Copyright:
© Academic Press 1994
Published:
Imprint:
Academic Press
eBook ISBN:
9780323138963
Hardcover ISBN:
9780126683301

About the Series Volume Editors

John Stevens Series Volume Editor

Affiliations and Expertise

Toronto Western Hospital and University of Toronto, Toronto, Ontario, Canada

Linda Mills Series Volume Editor

Affiliations and Expertise

Toronto Western Hospital and University of Toronto, Toronto, Ontario, Canada

Judy Trogadis Series Volume Editor

Affiliations and Expertise

Toronto Western Hospital and University of Toronto, Toronto, Ontario, Canada