The integration of confocal microscopy and volume investigation has led to an unprecedented ability to examine spatial relationships between cellular structure and function. The goal of this book is to familiarize the reader with these new technologies and to demonstrate their applicability to a wide range of biological and clinical problems.

Key Features

@introbul:Key Features @bul:* Volume investigation * Three-dimensional reconstruction * Fluroescent probe design * Biological applications of confocal microscopy, including calcium imaging, receptor movement, and diagnostic pathology * Confocal data display and analysis * Twenty-eight pages of color


AUDIENCE: Cell biologists, molecular biologists, neurobiologists, and biomedical researchers.

Table of Contents

Preface. Confocal Microscopy: Practical Considerations: Introduction to Confocal Three-Dimensional Volume Investigation. Background Rejection and Optimization of Signal to Noise in Confocal Microscopy. Sources of Noise in Three-Dimensional Microscopical Data Sets. Simultaneous Ultraviolet and Visible Wavelength Confocal Microscopy. Fluorescent Labels for Confocal Microscopy. Display Methods for Gray-Scale, Voxel-Based Data Sets. Three-Dimensional Volume Reconstitution in Confocal Microscopy: Practical Considerations. Biological Applications of Confocal Microscopy: Imagine Ion Channels in Live Central Neurons Using Fluorescent Ligands. Imaging Ion Channels in Live Central Neurons Using Fluorescent Ligands II. Labeling of Cells and Tissues. Expression of G Protein-Coupled Receptors n Baculovirus/Sf9 Cells: Imaging Receptor Distribution by Confocal Fluorescence Microscopy. Dynamic Intracellular Calcium Compartments: Confocal Microscopy Using Fluo-3 in Cells and Organelles. Confocal Microscopy of Living Eggs and Embryos. Spatial Organization of Microtubules in PC12 Cells: Three-Dimensional Electron Microscopy and Confocal Microscopy Applied to Volume Investigation. Confocal Imaging of Living Neurons and Organelles. Confocal Microscopy in Diagnostic Cytology. Confocal Microscopy in Diagnostic Pathology. Alternative Approaches and Future Applications: Ultrathin Optical Sectioning and Dynamic Volume Investigation with Conventional Light Microscopy. Serial Electron Microscopy as an Alternative or Complement to Confocal Microscopy for the Study of Synapses and Dendritic Spines in the Central Nervous System. Confocal Scanning Opthal


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© 1994
Academic Press
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