The Zebrafish: Genetics, Genomics, and Transcriptomics - 4th Edition - ISBN: 9780128034743, 9780128034880

The Zebrafish: Genetics, Genomics, and Transcriptomics, Volume 135

4th Edition

Serial Volume Editors: Leonard Zon Monte Westerfield
eBook ISBN: 9780128034880
Hardcover ISBN: 9780128034743
Imprint: Academic Press
Published Date: 20th July 2016
Page Count: 604
Sales tax will be calculated at check-out Price includes VAT/GST
Price includes VAT/GST
× DRM-Free

Easy - Download and start reading immediately. There’s no activation process to access eBooks; all eBooks are fully searchable, and enabled for copying, pasting, and printing.

Flexible - Read on multiple operating systems and devices. Easily read eBooks on smart phones, computers, or any eBook readers, including Kindle.

Open - Buy once, receive and download all available eBook formats, including PDF, EPUB, and Mobi (for Kindle).

Institutional Access

Secure Checkout

Personal information is secured with SSL technology.

Free Shipping

Free global shipping
No minimum order.

Table of Contents

Part 1. Forward and Reverse Genetics
1. Multiplex Conditional Mutagenesis in Zebrafish Using the CRISPR/Cas System
2. Tol2-Mediated Transgenesis, Gene Trapping and Enhancer Trapping, and the Gal4-UAS System
3. Genetic Screens for Mutations Affecting Adult Traits and Parental-Effect Genes
4. Targeted Candidate Gene Screens Using CRISPR/Cas9 Technology
5. TALEN and CRISPR Enhanced DNA Homologous Recombination for Gene Editing in Zebrafish
6. Precise Genome Editing by Homologous Recombination
7. The Zebrafish Genome Editing Toolkit
8. Clonal Analysis of Gene Loss-of-function and Tissue-specific Gene Deletion in Zebrafish via CRISPR/Cas9 Technology
9. Tissue-Specific Gene Targeting Using CRISPR/Cas9
Part 2. Transgenesis and Functional Genomics
10. Transcriptional Regulation Using the Q System in Transgenic Zebrafish
11. Contemporary Zebrafish Transgenesis with Tol2 and Application for Cre/lox Recombination Experiments
12. Method  for Somatic Cell Nuclear Transfer in Zebrafish
13. Experimental Approaches to Studying the Nature and Impact of Splicing Variation in Zebrafish
14. PICCORO: A Technique for Manipulating the Activity of Transcription Factors with Blue Light
Part 3. Transcriptomics
15. Tomo-seq: A Method to Obtain Genome-Wide Expression Data with Spatial Resolution
16. Cell-Type Specific Transcriptomic Analysis by Thiouracil Tagging in Zebrafish
17. RT-qPCR Gene Expression Analysis in Zebrafish: Preanalytical Precautions and Use of Expressed Repetitive Elements (ERE) for Normalization
Part 4. Epigenetics
18. Genome-Wide DNA Methylation Profiling in Zebrafish
19. Histone Modifications in Zebrafish Development
20. Chromatin Immunoprecipitation and an Open Chromatin Assay in Zebrafish Erythrocytes
21. Assay for Transposase-Accessible Chromatin and Circularized Chromosome Conformation Capt


The Zebrafish: Genetics, Genomics, and Transcriptomics, Fourth Edition, is the latest volume in the Methods in Cell Biology series that looks at methods for the analysis of genetics, genomics, and transcriptomics of Zebrafish.

Chapters cover such topics as gene-trap mutagenesis, genetic Screens for mutations, gene editing in zebrafish, homologous gene targeting, genome-wide RNA tomography, and developmental epigenetics and the zebrafish interactome.

Key Features

  • Covers sections on model systems and functional studies, imaging-based approaches, and emerging studies
  • Presents chapters written by experts in the field
  • Contains cutting-edge material on the topic


Researchers and students in cell, molecular and developmental biology


No. of pages:
© Academic Press 2016
Academic Press
eBook ISBN:
Hardcover ISBN:


Praise for the Series:
"The series is invaluable for workers at all levels of cell biology." --Nature

Ratings and Reviews

About the Serial Volume Editors

Leonard Zon Serial Volume Editor

Grousbeck Professor of Pediatrics, Boston Children's Hospital / HHMI, Boston, MA, USA

Affiliations and Expertise

Boston Children's Hospital / HHMI, Boston, MA, USA

Monte Westerfield Serial Volume Editor

Professor, Department of Biology, Institute of Neuroscience, University of Oregon, Eugene, OR, USA

Affiliations and Expertise

Institute of Neuroscience, University of Oregon, Eugene, OR, USA