The Zebrafish: Genetics, Genomics and Informatics, Volume 135

3rd Edition

Serial Volume Editors: H. William Detrich, III
Hardcover ISBN: 9780123748140
eBook ISBN: 9780080888576
Imprint: Academic Press
Published Date: 14th September 2011
Page Count: 528
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Table of Contents



Chapter 1: Generating Conditional Mutations in Zebrafish Using Gene-trap Mutagenesis

I. Introduction

II. Rationale for Conditional Mutations

III. Rationale for Gene-trap Mutagenesis to Generate Conditional Mutations

IV. Rationale for Vector Design

V. Methods

VI. Discussion

VII. Summary

Chapter 2: Tol2-mediated Transgenesis, Gene Trapping, Enhancer Trapping, and the Gal4-UAS System

I. Introduction

II. Transgenesis by Using the Tol2 Transposable Element

III. Tol2-mediated BAC Transgenesis

IV. Gene Trapping and Enhancer Trapping with the Tol2 Transposon System

V. Gene Trapping and Enhancer Trapping with the Gal4FF-UAS System

Chapter 3: Engineering Zinc Finger Nucleases for Targeted Mutagenesis of Zebrafish

I. Introduction

II. Methods and Resources for Engineering Zinc Finger Nucleases

III. Choice of Method to Engineer Zinc Finger Nucleases

Chapter 4: Retroviral-mediated Insertional Mutagenesis in Zebrafish

I. Introduction

II. Mutagenesis

III. Cloning the Mutated Genes

IV. Retroviral-mediated Insertional Mutagenesis as a Reverse Genetic Tool

V. Future Directions

Chapter 5: Genetic Screens for Mutations Affecting Adult Traits and Parental-effect Genes

I. Introduction

II. Strategies for Adult Trait and Parental-effect Screens

III. Selection of Lines for Genetic Screens

IV. Recovery and Maintenance of Adult and Parental-effect Mutations

V. Mapping Adult and Parental-effect Mutations

VI. Solutions, Materials, and Protocols

VII. Conclusions

Chapter 6: High-Throughput Target-Selected Gene Inactivation in Zebrafish

I. Introduction

II. Mutagenesis and Library Creation

III. PCR and Re-sequencing of Exons

IV. Analysis of Re-sequencing Data and Retrieval of M


This volume on genetics, genomics, and informatics, will cover new technologies in forward and reverse genetics, transgenesis, the zebrafish genome and mapping technologies, informatics and comparative genomics, and Infrastructure. This volume of Methods in Cell Biology will prove valuable both to seasoned zebrafish investigators as well as to those who are newly adopting the zebrafish model as part of their research armamentarium.


Seasoned zebrafish investigators as well as to those who are newly adopting the zebrafish model as part of their research armamentarium


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© Academic Press 2011
Academic Press
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About the Serial Volume Editors

H. William Detrich, III Serial Volume Editor

Professor of Biochemistry and Marine Biology at Northeastern University, promoted 1996. Joined Northeastern faculty in 1987. Previously a faculty member in Dept. of Biochemistry at the University of Mississippi Medical Center, 1983-1987.Principal Investigator in the U.S. Antarctic Program since 1984. Twelve field seasons "on the ice" since 1981. Research conducted at Palmer Station, Antarctica, and McMurdo Station, Antarctica.Research areas: Biochemical, cellular, and physiological adaptation to low and high temperatures. Structure and function of cytoplasmic microtubules and microtubule-dependent motors from cold-adapted Antarctic fishes. Regulation of tubulin and globin gene expression in zebrafish and Antarctic fishes. Role of microtubules in morphogenesis of the zebrafish embryo. Developmental hemapoiesis in zebrafish and Antarctic fishes. UV-induced DNA damage and repair in Antarctic marine organisms.

Affiliations and Expertise

Northeastern University, Boston, MA, USA