The Zebrafish: Disease Models and Chemical Screens

The Zebrafish: Disease Models and Chemical Screens

3rd Edition - September 9, 2011
  • Editor: H. Detrich, III
  • Hardcover ISBN: 9780123813206
  • eBook ISBN: 9780123813213

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This volume of Methods in Cell Biology is the 3e, and provides comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 2004. This new volume on Disease Models and Chemical Screens, covers two rapidly emerging and compelling applications of the zebrafish.

Key Features

  • Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls
  • This volume concentrates on Disease Models and Chemical Screens


Developmental biologists, neurobiologists, and cell biologists

Table of Contents

  • Contributors

    Chapter 1: Zebrafish Models of Germ Cell Tumor

    I. Overview

    II. Germline Development

    III. Germ Cell Tumors

    IV. Methods for Studying Zebrafish Germ Cells

    Chapter 2: Dissecting Mechanisms of Myelinated Axon Formation Using Zebrafish

    I. Introduction

    II. Methods and Materials

    III. Summary

    Chapter 3: In vivo Analysis of White Adipose Tissue in Zebrafish

    I. Introduction

    II. Rationale

    III. Materials

    IV. Methods

    V. Summary

    Chapter 4: Study of Host–Microbe Interactions in Zebrafish

    I. Introduction

    II. Laboratory Protocols

    Chapter 5: Hematopoietic Stem Cell Development: Using the Zebrafish to Identify the Signaling Networks and Physical Forces Regulating Hematopoiesis

    I. Developmental Hematopoiesis

    II. Review of the Literature

    III. Use of Zebrafish to Investigate HSC Biology

    IV. Zebrafish Tools and Protocols

    Chapter 6: Zebrafish as a Model for Hemorrhagic Stroke

    I. Introduction and Basic Concepts

    II. Maintaining the Barrier Function of Blood Vessels

    III. Vascular Integrity and Stroke

    IV. Zebrafish as a Model for Studying Hemorrhage and Stroke

    V. Studying Vascular Integrity in the Zebrafish

    VI. Concluding Remarks

    Chapter 7: A Zebrafish Model for VHL and Hypoxia Signaling

    I. VHL Disease

    II. Chuvash Polycythemia

    III. VHL Regulates HIF Signaling

    IV. HIF-Independent Functions of pVHL

    V. VHL Animal Models

    VI. Zebrafish as a New Model for VHL

    VII. Loss of Vhl Leads to a Systemic Hypoxic Response in Zebrafish

    VIII. vhl Mutants Develop Chuvash Polycythemia and Blood Cell Maturation Defects

    IX. Zebrafish vhl Mutants Develop Angiogenesis Defects

    X. Zebrafish vhl Mutants Develop Pronephros Abnormalities

    XI. Translation from Fish to Humans

    XII. Concluding Remarks

    Chapter 8: Basement Membrane Diseases in Zebrafish

    I. Introduction

    II. Basement Membrane-Related Zebrafish Pathologies

    III. Methodology for Zebrafish Studies of Basement Membrane Composition and Function

    IV. Summary

    Chapter 9: Zebrafish Provides a Novel Model for Lymphatic Vascular Research

    I. Introduction

    II. Embryonic Lymphangiogenesis in Zebrafish

    III. Comparison Between Teleosts and Other Vertebrates

    IV. The Added Value of Zebrafish

    V. The Secondary Vessel System in Teleosts

    VI. Concluding Remarks

    VII. Methods

    Chapter 10: Not All Bones are Created Equal – Using Zebrafish and Other Teleost Species in Osteogenesis Research

    I. Case Studies – Using Zebrafish for Addressing Biomedical Questions

    II. The Evolution of Skeletal Tissues

    III. Cartilage and Bone in Teleost Fish

    IV. Intermediate Skeletal Tissues

    V. Osteocyte-Containing Bone and Acellular Bone

    VI. Development of Teleost Vertebral Bodies, A Derived Process

    VII. Remodeling of the Teleost Skeleton

    VIII. Conclusions

    Chapter 11: Zebrafish Assays of Ciliopathies

    I. Introduction

    II. Methods

    III. Zebrafish Ciliary Mutant Lines

    IV. General Considerations/Future Development

    Chapter 12: Infectious Disease Modeling and Innate Immune Function in Zebrafish Embryos

    I. Introduction

    II. Observation and Isolation of Innate Immune Cells

    III. Bacterial Infection Methods

    IV. Analysis of the Innate Immune Response

    V. Conclusions

    Chapter 13: Zebrafish as a Model for the Study of Human Cancer

    I. Introduction: Zebrafish as a Cancer Model

    II. The Genetically Tractable Zebrafish

    III. Transgenic Models of Oncogenesis

    IV. Modeling the Loss of Tumor Suppression

    V. Modeling Tumor Cell Intravasation and Metastasis

    VI. Tumor-Initiating Cells

    VII. In vivo Small-Molecule Screens and Drug Discovery

    VIII. Conclusions

    Chapter 14: Generating and Analyzing Fish Models of Melanoma

    I. Introduction

    II. Transgenic Melanoma Lines in Zebrafish and Medaka

    III. Ultra-violet Irradiation Treatments

    IV. Basic Melanoma Pathology

    V. Histology

    VI. Molecular Analysis

    VII. RNA and Protein Isolation From Adult Fish Tumors

    VIII. Conclusions

    Chapter 15: Screening Pancreatic Oncogenes in Zebrafish Using the Gal4/UAS System

    I. Introduction

    II. Transgenic Zebrafish with Gal4/UAS-Mediated eGFP-KRASG12V Expression in the Exocrine Pancreas

    III. Identification and Characterization of Pancreatic Tumors

    IV. Conclusions

    Chapter 16: Zebrafish Models of Rhabdomyosarcoma

    I. Introduction

    II. Rationale

    III. Material and Methods

    IV. Discussion

    Chapter 17: Transplantation in Zebrafish

    I. Introduction

    II. Rationale

    III. Methods

    IV. Discussion

    Chapter 18: Disease Modeling by Gene Targeting Using MicroRNAs

    I. Introduction

    II. Mechanisms of MicroRNA Silencing

    III. Dicer and MicroRNAs in Zebrafish Development

    IV. Development of Vector-Based RNA Interference

    V. RNA Interference Work in Zebrafish

    VI. Use of siRNAs in the Zebrafish

    VII. Materials and Methods

    VIII. Results

    IX. Future Directions

    Chapter 19: Fluorescent Imaging of Cancer in Zebrafish

    I. Introduction

    II. Fluorescent Proteins and Transgenic Models of Cancer

    III. Macroscopic Observation of Tumor Growth

    IV. Microscopic Observation in Tumorigenesis

    V. Confirming Transformation of Fluorescent-Labeled Tumor Cells by Cell Transplantation into Irradiated Recipient Animals

    VI. Identifying Tumor-Propagating Cell Subpopulations by Fluorescent Protein Expression and Cell Transplantation into Irradiated Recipient Animals

    VII. Use of Syngeneic Zebrafish for Cell Transplantation of Fluorescent-Labeled Tumors and Drug Discovery

    VIII. Cell Transplantation into Syngeneic Zebrafish to Accurately Assess Self-Renewal in Fluorescent-Labeled Cancer

    IX. Use of Syngeneic Zebrafish for Cell Transplantation: Single Cell Transplants

    X. Xenograft Transplantation of Fluorescently Labeled Cells into Zebrafish

    XI. Conclusions

    Chapter 20: The Role of Fanconi Anemia/BRCA Genes in Zebrafish Sex Determination

    I. Introduction

    II. Results and Discussion

    III. Summary

    Chapter 21: Chemical Screening in Zebrafish for Novel Biological and Therapeutic Discovery

    I. Introduction

    II. Rationale

    III. Materials and Methods

    IV. Discussion/Caveats

    V. Summary

    Chapter 22: Using the Zebrafish Photomotor Response for Psychotropic Drug Screening

    I. Introduction

    II. The PMR Behavior

    III. Methods

    IV. Discussion

    Chapter 23: Designing Zebrafish Chemical Screens

    I. Rationale for Conducting Small Molecule Screens With Zebrafish

    II. Selection of Small Molecule Libraries

    III. Assay Design: Major Screen Types

    IV. Screening Methods

    V. Mechanism of Action Studies

    VI. Conclusions



    Volumes in Series

Product details

  • No. of pages: 616
  • Language: English
  • Copyright: © Academic Press 2011
  • Published: September 9, 2011
  • Imprint: Academic Press
  • Hardcover ISBN: 9780123813206
  • eBook ISBN: 9780123813213

About the Serial Volume Editor

H. Detrich, III

Professor of Biochemistry and Marine Biology at Northeastern University, promoted 1996. Joined Northeastern faculty in 1987. Previously a faculty member in Dept. of Biochemistry at the University of Mississippi Medical Center, 1983-1987.Principal Investigator in the U.S. Antarctic Program since 1984. Twelve field seasons "on the ice" since 1981. Research conducted at Palmer Station, Antarctica, and McMurdo Station, Antarctica.Research areas: Biochemical, cellular, and physiological adaptation to low and high temperatures. Structure and function of cytoplasmic microtubules and microtubule-dependent motors from cold-adapted Antarctic fishes. Regulation of tubulin and globin gene expression in zebrafish and Antarctic fishes. Role of microtubules in morphogenesis of the zebrafish embryo. Developmental hemapoiesis in zebrafish and Antarctic fishes. UV-induced DNA damage and repair in Antarctic marine organisms.

Affiliations and Expertise

Northeastern University, Boston, MA, USA