This volume of Methods in Cell Biology is the 3e, and provides comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 2004. This new volume on Disease Models and Chemical Screens, covers two rapidly emerging and compelling applications of the zebrafish.

Key Features

* Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls
* This volume concentrates on Disease Models and Chemical Screens


Developmental biologists, neurobiologists, and cell biologists.

Table of Contents


Chapter 1: Zebrafish Models of Germ Cell Tumor

I. Overview

II. Germline Development

III. Germ Cell Tumors

IV. Methods for Studying Zebrafish Germ Cells

Chapter 2: Dissecting Mechanisms of Myelinated Axon Formation Using Zebrafish

I. Introduction

II. Methods and Materials

III. Summary

Chapter 3: In vivo Analysis of White Adipose Tissue in Zebrafish

I. Introduction

II. Rationale

III. Materials

IV. Methods

V. Summary

Chapter 4: Study of Host–Microbe Interactions in Zebrafish

I. Introduction

II. Laboratory Protocols

Chapter 5: Hematopoietic Stem Cell Development: Using the Zebrafish to Identify the Signaling Networks and Physical Forces Regulating Hematopoiesis

I. Developmental Hematopoiesis

II. Review of the Literature

III. Use of Zebrafish to Investigate HSC Biology

IV. Zebrafish Tools and Protocols

Chapter 6: Zebrafish as a Model for Hemorrhagic Stroke

I. Introduction and Basic Concepts

II. Maintaining the Barrier Function of Blood Vessels

III. Vascular Integrity and Stroke

IV. Zebrafish as a Model for Studying Hemorrhage and Stroke

V. Studying Vascular Integrity in the Zebrafish

VI. Concluding Remarks

Chapter 7: A Zebrafish Model for VHL and Hypoxia Signaling

I. VHL Disease

II. Chuvash Polycythemia

III. VHL Regulates HIF Signaling

IV. HIF-Independent Functions of pVHL

V. VHL Animal Models

VI. Zebrafish as a New Model for VHL

VII. Loss of Vhl Leads to a Systemic Hypoxic Response in Zebrafish

VIII. vhl Mutants Develop Chuvash Polycythemia and Blood Cell Maturation Defects

IX. Zebrafish vhl Mutants Develop Angiogenesis Defects

X. Zebrafish vhl Mutants Develop Pronephros Abnormalities

XI. Translation from Fish to Humans


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© 2011
Academic Press
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