The Zebrafish: Cellular and Developmental Biology, Part B

The Zebrafish: Cellular and Developmental Biology, Part B

3rd Edition - April 29, 2011
  • Editors: H. Detrich, III, Monte Westerfield, Leonard Zon
  • Hardcover ISBN: 9780123870360
  • eBook ISBN: 9780123870377

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This volume of Methods in Cell Biology, the second of two parts on the subject of zebrafish, provides a comprehensive compendium of laboratory protocols and reviews covering all the new methods developed since 1999.

Key Features

  • Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls
  • Illustrates many techiques in full-color
  • Summarizes the Zebrafish Genome Project


Developmental biologists, neurobiologists, and cell biologists

Table of Contents

  • Live Imaging of the Cytoskeleton in Early Cleavage-Stage Zebrafish Embryos

    I. Introduction

    II. Maintaining the Breeding Competence of Zebrafish throughout the Day

    III. Mounting Zebrafish Embryos for Live Imaging

    IV. Live Imaging of Microtubules in Cleaving Zebrafish Embryos

    V. Live Imaging of Microfilaments in Cleaving Zebrafish Embryos

    VI. Comparison of Microscopic Techniques for Imaging the Cytoskeleton of Cleaving Zebrafish Embryos

    VII. Discussion and Future Directions


    Appendix A. Supplementary Movies

    Appendix A. Supplementary Movies

    Analysis of Cell Proliferation, Senescence, and Cell Death in Zebrafish Embryos

    I. Introduction: The Cell Cycle in Zebrafish

    II. Zebrafish Embryo Cell-Cycle Protocols

    III. Screening for Chemical Suppressors of Zebrafish Cell-Cycle Mutants

    IV. Conclusions

    V. Reagents and Supplies


    Analysis of Cilia Structure and Function in Zebrafish

    I. Introduction

    II. Cilia in Zebrafish Embryos and Larvae

    III. Analytical Tools for Cilia Morphology and Motility

    IV. Analysis of Cilia-related Mutant Phenotypes in Zebrafish

    V. Future Directions


    Cellular Dissection of Zebrafish Hematopoiesis

    I. Introduction

    II. Zebrafish Hematopoiesis

    A. Primitive Hematopoiesis

    III. Hematopoietic Cell Transplantation

    IV. Enrichment of Hematopoietic Stem Cells

    V. In vitro Culture and Differentiation of Hematopoietic Progenitors

    VI. Conclusions

    Zebrafish Lipid Metabolism: From Mediating Early Patterning to the Metabolism of Dietary Fat and Cholesterol

    I. Introduction

    II. Lipid Metabolism in Developing Zebrafish

    III. Yolk Metabolism During Early Vertebrate Development

    IV. Lipid Signaling During Early Zebrafish Development

    V. Visualizing Lipid Metabolism in Larval and Adult Zebrafish

    VI. Triple Screen: Phospholipase, Protease and Swallowing Function Assays

    VII. Zebrafish Models of Human Dyslipidemias

    VIII. Summary


    Development of the Zebrafish Enteric Nervous System

    I. Introduction

    II. Organization of the Zebrafish Intestinal Tract

    III. Early Development of the ENS

    IV. Genetic Approaches to Studying ENS Development

    V. Molecular Mechanisms of ENS Development

    VI. ENS Differentiation

    VII. Regulation of Gut Motility

    VIII. Zebrafish ENS as a Model for Understanding Human Diseases

    IX. Future Prospects


    A Guide to Analysis of Cardiac Phenotypes in the Zebrafish Embryo

    I. Introduction

    II. Defects in Heart Size

    III. Defects in Heart Shape

    IV. Defects in Cardiac Function

    V. Summary

    Chemical Approaches to Angiogenesis in Development and Regeneration

    I. Introduction

    II. Chemical Approaches and Zebrafish Vascular Development

    III. Novel Chemical Strategies to Investigate Mechanisms of Angiogenesis

    IV. Summary


    Laser-Induced Thrombosis in Zebrafish

    I. Introduction

    II. Vascular Occlusion

    III. Methods

    IV. Future Perspectives

    Endoderm Specification, Liver Development, and Regeneration

    I. Review of the Literature

    II. Embryonic and Larval Protocols to Analyze Liver Formation

    III. Liver Injury and Regeneration Protocols

    IV. Assessment of Liver Function

    V. Summary

    Morphogenesis of the Zebrafish Jaw: Development Beyond the Embryo

    I. Postembryonic Development – Framing the Questions and Understanding the Challenges

    II. Obtaining Phenotypes

    III. Quantitative Methods for Studying Adult Phenotypes

    IV. A Complementary Approach: The Use of Natural Variation to Complement that Generated in the Lab for Understanding Jaw Morphogenesis

    V. Implications and Conclusions

    Associative Learning in Zebrafish (Danio rerio)

    I. Introduction

    II. Rationale

    III. Methods and Discussion

    IV. Summary


Product details

  • No. of pages: 312
  • Language: English
  • Copyright: © Academic Press 2011
  • Published: April 29, 2011
  • Imprint: Academic Press
  • Hardcover ISBN: 9780123870360
  • eBook ISBN: 9780123870377

About the Serial Volume Editors

H. Detrich, III

Professor of Biochemistry and Marine Biology at Northeastern University, promoted 1996. Joined Northeastern faculty in 1987. Previously a faculty member in Dept. of Biochemistry at the University of Mississippi Medical Center, 1983-1987.Principal Investigator in the U.S. Antarctic Program since 1984. Twelve field seasons "on the ice" since 1981. Research conducted at Palmer Station, Antarctica, and McMurdo Station, Antarctica.Research areas: Biochemical, cellular, and physiological adaptation to low and high temperatures. Structure and function of cytoplasmic microtubules and microtubule-dependent motors from cold-adapted Antarctic fishes. Regulation of tubulin and globin gene expression in zebrafish and Antarctic fishes. Role of microtubules in morphogenesis of the zebrafish embryo. Developmental hemapoiesis in zebrafish and Antarctic fishes. UV-induced DNA damage and repair in Antarctic marine organisms.

Affiliations and Expertise

Northeastern University, Boston, MA, USA

Monte Westerfield

Professor, Department of Biology, Institute of Neuroscience, University of Oregon, Eugene, OR, USA

Affiliations and Expertise

Institute of Neuroscience, University of Oregon, Eugene, OR, USA

Leonard Zon

Grousbeck Professor of Pediatrics, Boston Children's Hospital / HHMI, Boston, MA, USA

Affiliations and Expertise

Boston Children's Hospital / HHMI, Boston, MA, USA