The Neuronal Cytoskeleton, Motor Proteins, and Organelle Trafficking in the Axon

The Neuronal Cytoskeleton, Motor Proteins, and Organelle Trafficking in the Axon

1st Edition - January 12, 2016

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  • Editor: K. Kevin Pfister
  • eBook ISBN: 9780128033548
  • Hardcover ISBN: 9780128033449

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The Neuronal Cytoskeleton, Motor Proteins, and Organelle Trafficking in the Axon, a new volume in the Methods in Cell Biology series continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in neuronal cells, and includes sections on such topics as actin transport in axons and neurofilament transport.

Key Features

  • Covers an increasingly appreciated field in cell biology
  • Includes both established and new technologies
  • Contributed by experts in the field


Researchers and students in cell, molecular and developmental biology.

Table of Contents

  • 1. Axonal Transport: The Orderly Motion of Axonal Structures
    Mark M Black
    2. Live-Cell Imaging of Neurofilament Transport in Cultured Neurons
    Atsuko Uchida, Paula C. Monsma, J. Daniel Fenn, Anthony Brown
    3. Axonal Actin in Action: Imaging Actin Dynamics in Neurons
    Kelsey Ladt, Archan Ganguly, Subhojit Roy
    4. Live Imaging of Microtubule Dynamics in Organotypic Hippocampal Slice Cultures
    Philipp Schätzle, Lukas C. Kapitein, Casper C. Hoogenraad
    5. Studying Neuronal Microtubule Organization and Microtubule-Associated Proteins Using Single Molecule Localization Microscopy
    Anaël Chazeau, Eugene A. Katrukha, Casper C. Hoogenraad, Lukas C. Kapitein
    6. The Use of Proteomic Analysis to Study Trafficking Defects in Axons
    Xiaoqin Fu, Kristy J. Brown, Sree Rayavarapu, Kanneboyina Nagaraju, Judy S. Liu
    7. Using siRNA to Study Microtubule-Related Proteins in Cultured Neurons
    Lanfranco Leo, Wenqian Yu, Peter W. Baas
    8. Optimization of Cerebellar Purkinje Neuron Cultures and Development of a Plasmid-Based Method for Purkinje Neuron-Specific, miRNA-Mediated Protein Knockdown
    C. J. Alexander, J. A. Hammer III
    9. Biochemical Analysis of Axon-Specific Phosphorylation Events Using Isolated Squid Axoplasms
    Minsu Kang, Lisa Baker, Yuyu Song, Scott Brady, Gerardo Morfini
    10. Analyzing Kinesin Motor Domain Translocation in Cultured Hippocampal Neurons
    Rui Yang, Marvin Bentley, Chung-Fang Huang, Gary Banker
    11. Imaging and Quantitative Analysis of Cytokinesis in Developing Brains of Kinesin-6 Mutant Mice
    Kerstin M. Janisch, Noelle D. Dwyer
    12. Live Cell Imaging of Cytoplasmic Dynein Movement in Transfected Embryonic Rat Neurons
    Mitchell W. Ross, David J. Mitchell, John C. Cain, Kiev R. Blasier, K. Kevin Pfister
    13. Live-Cell Imaging of Retrograde Transport Initiation in Primary Neurons
    Jeffrey J. Nirschl, Erika L. F. Holzbaur
    14. Methods to Identify and Analyze Gene Products Involved in Neuronal Intracellular Transport Using Drosophila
    Amanda L. Neisch, Adam W. Avery, James B. Machamer, Min-gang Li, Thomas S. Hays
    15. In vivo Analysis of Axonal Transport in Zebrafish
    Catherine M Drerup, Alex V Nechiporuk
    16. Fast Axonal Transport in Isolated Axoplasm from the Squid Giant Axon
    Yuyu Song, Minsu Kang, Gerardo Morfini, Scott T. Brady
    17. Cellular and Subcellular Imaging of Motor Protein-Based Behavior in Embryonic Rat Brain
    Alexandre D. Baffet, Aurélie Carabalona, Tiago J. Dantas, David D. Doobin, Daniel J. Hu, Richard B. Vallee
    18. Tracking Quantum-Dot Labeled Neurotropic Factors Transport Along Primary Neuronal Axons in Compartmental Microfluidic Chambers
    Shani Gluska, Michael Chein, Nimrod Rotem, Ariel Ionescu, Eran Perlson
    19. Studying Endosomes in Cultured Neurons by Live-Cell Imaging
    Zofia M. Lasiecka, Bettina Winckler
    20. Comparative Analysis of Axonal Transport Markers in Primary Mammalian Neurons
    Eva Klinman, Erika L. F. Holzbaur
    21. Imaging Organelle Transport in Primary Hippocampal Neurons Treated with Amyloid-β Oligomers
    Kathlyn J. Gan, Michael A. Silverman
    22. High Spatial and Temporal Resolution Imaging of Motor-dependent Transport in Neuronal and Nonneuronal Cells
    Julie Yi, Noopur V. Khobrekar, Tiago J. Dantas, Jie Zhou, Richard B. Vallee
    23. Isolation and Analyses of Axonal Ribonucleoprotein Complexes
    Ella Doron-Mandel, Stefanie Alber, Juan A. Oses, Katalin F. Medzihradszky, Alma L. Burlingame, Mike Fainzilber, Jeffery L. Twiss, Seung Joon Le

Product details

  • No. of pages: 534
  • Language: English
  • Copyright: © Academic Press 2016
  • Published: January 12, 2016
  • Imprint: Academic Press
  • eBook ISBN: 9780128033548
  • Hardcover ISBN: 9780128033449

About the Serial Volume Editor

K. Kevin Pfister

Associate Professor, Cell Biology Department, University of Virginia, USA

Affiliations and Expertise

Cell Biology Department, University of Virginia, USA

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