The Detection of Biomarkers

Past, Present, and the Future Prospects

1st Edition - December 5, 2021
Editors: Sibel A. Ozkan, Nurgul K. Bakirhan, Fariba Mollarasouli
Language: English
Paperback ISBN:
9 7 8 - 0 - 1 2 - 8 2 2 8 5 9 - 3
eBook ISBN:
9 7 8 - 0 - 1 2 - 8 2 3 0 7 5 - 6

Reliable, precise and accurate detection and analysis of biomarkers remains a significant challenge for clinical　researchers. Methods for the detection of biomarkers are rather co… Read more

Purchase options

LIMITED OFFER

Save 50% on book bundles

Immediately download your ebook while waiting for your print delivery. No promo code is needed.

Institutional subscription on ScienceDirect

Request a sales quote

Reliable, precise and accurate detection and analysis of biomarkers remains a significant challenge for clinical　researchers. Methods for the detection of biomarkers are rather complex, requiring pre-treatment steps before analysis can take place. Moreover, comparing various biomarker assays and tracing research progress in this area systematically is a challenge for researchers. The Detection of Biomarkers presents developments in biomarker detection, including methods tools and strategies, biosensor design, materials, and applications. The book presents methods, materials and procedures that are simple, precise, sensitive, selective, fast and economical, and therefore highly practical for use in clinical research scenarios. This volume situates biomarker detection in its research context and sets out future prospects for the area. Its 20 chapters offer a comprehensive coverage of biomarkers, including progress on nanotechnology, biosensor types, synthesis, immobilization, and applications in various fields. The book also demonstrates, for students, how to synthesize and immobilize biosensors for biomarker assay. It offers researchers real alternative and innovative ways to think about the field of biomarker detection, increasing the reliability, precision and accuracy of biomarker detection.

Cover image
Title page
Table of Contents
Copyright
List of contributors
Preface
Chapter 1. Introduction to biomarkers
Abstract
1.1 Introduction
1.2 Classification of biomarkers
1.3 Biomarker discovery and diagnosis techniques and strategies
1.4 Biomarker validation
1.5 Biological matrices used for detection
1.6 Applications of biomarkers in medicine
1.7 Future prospects
Acknowledgement
References
Chapter 2. Low-molecular-weight biomarkers: types and detection strategies
Abstract
2.1 Introduction
2.2 Type of low-molecular-weight biomarkers
2.3 Low-molecular-weight detection strategies
2.4 Bioapplications of low-molecular-weight detection
2.5 Conclusion
References
Chapter 3. Circulating miRNAs as biomarkers for noninvasive cancer diagnosis
Abstract
3.1 Introduction
3.2 An overview of miRNAs
3.3 Definition of biosensors
3.4 Optical biosensors
3.5 Colorimetric biosensors
3.6 Fluorescence-based biosensors
3.7 Chemiluminescence- and electrochemiluminescent-based biosensors
3.8 Surface plasmon resonance-based biosensors
3.9 Electrochemical biosensors
3.10 Future perspectives
Acknowledgments
References
Chapter 4. Cardiac biomarkers: definition, detection, diagnostic use, and efficiency
Abstract
4.1 Cardiac biomarkers of the acute coronary syndrome
4.2 Biomarkers of cardiac failure
4.3 Biomarkers of coronary heart disease
4.4 Conclusion
References
Chapter 5. Proteomic-based identification of novel biomarkers
Abstract
5.1 Introduction
5.2 Designing a biomarker discovery study
5.3 Methodologies for proteomic-based biomarker discovery studies
5.4 The current state of proteomic-based biomarkers
5.5 Clinical biomarkers discovered by proteomic approaches
5.6 Limitations
5.7 Future perspectives
References
Chapter 6. Metabolomics tools for biomarker discovery: applications in chronic kidney disease
Abstract
6.1 Introduction
6.2 Metabolomics tools for the discovery of novel renal disease biomarkers
References
Chapter 7. Electronic devices for biomarker monitoring
Abstract
7.1 Introduction
7.2 Biomarkers
7.3 Sensing electronic devices: transistors
7.4 Conclusion and perspectives
References
Chapter 8. Novel advances in nanomaterial-based electrochemical sensing of the biomarker
Abstract
8.1 Introduction
8.2 Classification of biomarkers
8.3 Various nanomaterials as signal amplification
8.4 The novel advance of immobilization strategies for biomarkers
8.5 Trends research on electrochemical nano-biosensing of the biomarkers
8.6 Conclusion and future perspectives
References
Chapter 9. Carbon nanomaterials-based electrochemical cancer biomarkers biosensors
Abstract
9.1 General concepts
9.2 Carbon nanostructures-based electrochemical (bio)sensors for the quantification of cancer biomarkers
9.3 Conclusions and perspectives
Acknowledgments
References
Chapter 10. Optical nanosensor based on surface-enhanced Raman spectroscopy for biomedical and biomarker detection applications
Abstract
10.1 Introduction
10.2 Raman spectroscopy techniques for biomedical applications
10.3 Surface-enhanced Raman spectroscopy-based applications in biomarkers
10.4 Surface-enhanced Raman spectroscopy applications in bioimaging
10.5 Raman activated cell sorting
10.6 Surface-enhanced Raman spectroscopy-based microchip system
10.7 Surface-enhanced Raman spectroscopy mapping
10.8 Conclusions and future prospects
References
Chapter 11. Optical smartphone-based sensing: diagnostic of biomarkers
Abstract
11.1 Introduction
11.2 Optical smartphone-based biosensors for the detection of biomarkers
11.3 Summary and future perspectives
Acknowledgments
References
Chapter 12. Design of immunosensors for rapid and sensitive detection of biomarkers
Abstract
12.1 Immunosensor
12.2 Biomarkers and their classification
12.3 Antibodies and their immobilization methods
12.4 Immunosensor formats
12.5 Applications of nanomaterials to amplify the analytical signal of immunosensors
12.6 Transducers used for immunosensors
12.7 Multiplexed assay–based immunosensors for biomarkers detection
12.8 Conclusions and future outlook
Acknowledgments
References
Chapter 13. Recent advances in immunosensors for healthcare
Abstract
13.1 Introduction
13.2 Immunosensors for healthcare
13.3 Summary and outlooks
References
Chapter 14. Impedimetric immunosensors for detection of biomarkers
Abstract
14.1 Introduction
14.2 Conclusion and future remarks
References
Chapter 15. Conducting polymers—versatile tools in analytical systems for the determination of biomarkers and biologically active compounds
Abstract
15.1 Introduction
15.2 Conclusions
Funding
Conflicts of interest
References
Chapter 16. Molecularly imprinted biosensors for sensitive detection of biomarkers
Abstract
16.1 Introduction
16.2 Biomarkers
16.3 Molecularly imprinting technology
16.4 Surface imprinting
16.5 Electrochemical transduction
16.6 Voltammetry/amperometry
16.7 Potentiometry
16.8 Capacitance/impedance
16.9 Optical sensors
16.10 Piezoelectric sensors
16.11 Conclusion
References
Chapter 17. Microfluidics technology: past, present, and future prospects for biomarker diagnostics
Abstract
17.1 General introduction
17.2 Type of microfluidics system
17.3 Application of microfluidic system
17.4 Future and perspectives
References
Chapter 18. Wearable biosensors for monitoring of disease-related biomarkers
Abstract
18.1 Introduction
18.2 Wearable (bio)devices for monitoring of disease biomarkers
18.3 Wearable optical (bio)sensors
18.4 Wearable electrochemical biosensors
18.5 Opportunities, challenges, concerns, and future prospects
Acknowledgments
References
Chapter 19. Nanomachines and nanorobotics: improving cancer diagnosis and therapy
Abstract
19.1 Introduction
19.2 Nanomachines at a glance
19.3 Nanodevices in cancer diagnosis and imaging
19.4 Nanomachines in active cancer drugs delivery
19.5 Nanomachines and nanorobots for cancer theragnostic
19.6 Nanosurgery
19.7 Conclusion and future prospects
Acknowledgments
References
Chapter 20. Analytical perspective on biomarkers research: from untargeted to targeted metabolomics
Abstract
20.1 Introduction
20.2 Krebs cycle targeted pathway analysis
20.3 Sulfur pathway
20.4 Phosphonucleotides
20.5 Validation of targeted metabolomic analysis
20.6 Conclusion
References
Index

Sibel A. Ozkan

Prof. Dr. Sibel A. Ozkan is a Full Professor in the Faculty of Pharmacy at Ankara University. She has over 30 years of experience in analytical chemistry. She has established expertise in electrochemistry, validation, electrochemical biosensors, DNA biosensors, enzyme biosensors, biomarkers, drug analysis from biological samples or dosage forms, liquid chromatography, capillary electrophoresis, spectrophotometry. She has won Encouragement Award from Ankara University in 2003, Science Award from Turkish Pharmacists Association in 2008, and The Best Ph.D. Thesis in Turkey Award 2017 (Health Sciences), High Council of Education of Turkey, Ton Duc Thang University “Woman in Science 2019” Prize, 27 December 2019, Ankara University Science Support Award, 2020.

Affiliations and expertise

Ankara University, Turkey

Nurgul K. Bakirhan

Nurgul K. Bakirhan is an Associated Professor at the Department of Analytical Chemistry, Gulhane Faculty of Pharmacy, University of Health Sciences, Turkey. She received her doctorate from the Faculty of Pharmacy at Ankara University in Turkey. She was visiting researcher at the Université libre de Bruxelles with TUBITAK 2214 fellowship during six months. Her research interests include sensors, nanomaterials, conductive polymers, electrochemical impedance spectroscopy and electrochemical investigations on active pharmaceutical compounds, validation. She has published over 60 papers and 10 book chapters.

Affiliations and expertise

Associated Professor at the Department of Analytical Chemistry, Gulhane Faculty of Pharmacy, University of Health Sciences, Turkey

Fariba Mollarasouli

Fariba Mollarasouli is an Assistant Professor in the Department of Chemistry at Yasouj University, Yasouj, Iran. Her areas of research and expertise include analytical and bioanalytical method development, drug analysis, electrochemical sensors and biosensors, immunosensors, as well as the development of various nanomaterials (design, synthesis, characterization, and applications) for fabricating nanosensors with clinical and environmental applications.

Affiliations and expertise

Assistant Professor in the Department of Chemistry at Yasouj University, Yasouj, Iran