The Cell Nucleus V1

The Cell Nucleus V1

1st Edition - January 28, 1974

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  • Editor: Harris Busch
  • eBook ISBN: 9780323143332

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Description

The Cell Nucleus, Volume I reports the basic concepts of cell nucleus, including nuclear structure, the interaction between the nucleus and cytoplasm, and the chromatin. This volume first describes the nucleus’ morphological structures and relates these structures to its functions. It then discusses nuclear organization in plant cells; morphology and biochemistry of the slime mold nucleus; and structure, function, and properties of nuclear envelope. In addition, it addresses the molecular movements between nucleus and cytoplasm against a concentration gradient, presents experiments with animal cell heterokaryons, and explains the genome in specialized cells. It also explores the organization of the chromatin fiber; the human chromosome structure before and after banding; and the ultrastructure and function of heterochromatin and euchromatin.

Table of Contents


  • List of Contributors

    Preface

    Contents of Other Volumes

    Introduction

    Part I Nuclear Structure

    Chapter 1 Localization of Nuclear Functions as Revealed by Ultrastructural Autoradiography and Cytochemistry

    I. Localization of Chromatin

    II. Sites of Replication

    III. Localization of Ribonucleoproteins

    IV. Sites of Transcription

    V. Nuclear Proteins

    VI. Discussion

    References

    Chapter 2 The Nucleolus and Nucleolar DNA

    I. Historical

    II. Variations in Nucleolar Structure

    III. The Role of the Nucleolus

    IV. The Ultrastructure of the Nucleolus

    V. Perinucleolar and Intranucleolar Chromatin Structures

    VI. Compact Nucleoli

    VII. Nucleoli with Nucleolonemas

    VIII. Ring-Shaped Nucleoli

    IX. Nucleolar Fibrillar and Granular Components

    X. Dense Intranucleolar Particles

    XI. The Nucleolar Microspherules

    XII. The Nucleolar Light Areas: Interstices and Vacuoles

    XIII. Nucleolar Fibrillar Centers and Nucleolini

    XIV. Natural Nucleolar Segregation

    XV. Nucleolar Constituents

    XVI. Synthesis and Cell Content of rDNA

    XVII. Summary

    References

    Chapter 3 Ultrastructural Organization of Plant Cell Nuclei

    I. Introduction

    II. Types of Interphase Nuclei

    III. Organization and Possible Role of Pufflike Chromosome Segments

    IV. Possible Factors Involved in Nuclear Structure Diversity

    V. Pattern of Organization of Plant Nucleoli

    References

    Chapter 4 The Nucleus of the Plasmodial Slime Molds

    I. Introduction

    II. Culture Methods and Gross Morphology

    III. Microscopic Morphology

    IV. Chromosome Numbers

    V. Isolation of Nuclei and Nucleoli

    VI. Biochemistry

    References

    Chapter 5 Structures and Functions of the Nuclear Envelope

    I. Introduction

    II. The Nuclear Envelope as a Means of Intracellular ComPartmentalization

    III. Methodological Progress

    IV. The Nuclear Envelope as a Part of the Endoplasmic Reticulum

    V. Associations of Inner Nuclear Membrane with Chromosomes and Chromatin

    VI. Association of the Inner Nuclear Membrane with Specific Proteinaceous Layers

    VII. The Pore Complex

    VIII. Relationship of the Nuclear Envelope to Annulate Lamellae (AL) and Intranuclear Cisternae

    IX. Structural Differentiation of the Nuclear Envelope

    X. Associations and Interactions of the Nuclear Envelope with Microtubules and Microfilaments

    XI. Relationship of the Nuclear Envelope to Other Cell Organelles

    XII. Breakdowns and Reconstitutions of the Nuclear Envelope; Role of the Nuclear Envelope in Nuclear Divisions and Fusions

    Note Added in Proof

    References

    Chapter 6 Chemical and Biochemical Properties of the Nuclear Envelope

    I. Introduction

    II. Isolation and Physical Properties

    III. Chemical Properties

    IV. Biochemical Properties

    V. Conclusions

    Note Added in Proof

    References

    Part II Nuclear-Cytoplasmic Interactions

    Chapter 7 Movement of Molecules between Nucleus and Cytoplasm

    I. Introduction

    II. Observations from Which It Can Be Inferred That Molecules Move Nonrandomly between Cytoplasm and Nucleus

    III. Unidirectional Movement of RNA from Nucleus to Cytoplasm

    IV. Unidirectional Movement of Proteins from Cytoplasm to Nucleus

    V. Movement of DNA from Nucleus to Cytoplasm

    VI. Some Proteins Shuttle Nonrandomly between Nucleus and Cytoplasm

    VII. RNA's That Shuttle Nonrandomly between Nucleus and Cytoplasm

    VIII. Movement of Ions between Nucleus and Cytoplasm

    IX. Conclusions

    References

    Chapter 8 Heterokaryons and Their Uses in Studies of Nuclear Function

    I. Introduction

    II. Methods of Inducing Cell Fusion

    III. Mechanism of Cell Fusion

    IV. Nuclear Reactivation

    V. Gene Expression

    VI. Heterokaryons in Virological Studies

    VII. Studies on Induction of DNA Synthesis, Mitosis, and Premature Chromosome Condensation

    VIII. Conclusion

    References

    Chapter 9 The Genome in Specialized Cells, as Revealed by Nuclear Transplantation in Amphibia

    I. Introduction

    II. Methods of Nuclear Transplantation

    III. Evidence for Genetic Identity of Transplanted Somatic Nuclei

    IV. Loss of Developmental Capacity of Transplanted Nuclei

    V. Conclusions on the Developmental Capacity of Transplanted Nuclei

    VI. General Conclusions

    References

    Part III Chromatin

    Chapter 10 The Molecular Organization of the Chromatin Fiber

    I. Introduction

    II. Morphological Methods for the Study of Chromatin Fibers

    III. Organization of Chromatin Fibers in Thin Sections

    IV. Organization of Chromatin Fibers Spread on Liquid Surfaces

    V. Composition of Spread Chromatin Fibers

    VI. Morphology of the DNA Moiety of Chromatin Fibers

    VII. Physical Studies on Nucleohistone and Chromatin

    VIII. Packing and Coiling of DNA: Models of the Chromatin Fiber

    References

    Chapter 11 The Structure of Human Chromosomes

    I. Chromosome Structure before Banding

    II. A Prebanding Model

    III. Chromosome Structure after Banding

    IV. Centromeric versus Intercalary Heterochromatin

    V. Mechanisms of Banding

    VI. Chromosome Structure after Banding

    References

    Chapter 12 Ultrastructure and Function of Heterochromatin and Euchromatin

    I. History of Heterochromatin and Euchromatin

    II. Ultrastructure of Heterochromatin and Euchromatin

    III. Function of Heterochromatin and Euchromatin

    IV. Summary

    References

    Author Index

    Subject Index




Product details

  • No. of pages: 694
  • Language: English
  • Copyright: © Academic Press 1974
  • Published: January 28, 1974
  • Imprint: Academic Press
  • eBook ISBN: 9780323143332

About the Editor

Harris Busch

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