The Cell Nucleus V1 - 1st Edition - ISBN: 9780121476014, 9780323143332

The Cell Nucleus V1

1st Edition

Editors: Harris Busch
eBook ISBN: 9780323143332
Imprint: Academic Press
Published Date: 28th January 1974
Page Count: 694
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The Cell Nucleus, Volume I reports the basic concepts of cell nucleus, including nuclear structure, the interaction between the nucleus and cytoplasm, and the chromatin. This volume first describes the nucleus’ morphological structures and relates these structures to its functions. It then discusses nuclear organization in plant cells; morphology and biochemistry of the slime mold nucleus; and structure, function, and properties of nuclear envelope. In addition, it addresses the molecular movements between nucleus and cytoplasm against a concentration gradient, presents experiments with animal cell heterokaryons, and explains the genome in specialized cells. It also explores the organization of the chromatin fiber; the human chromosome structure before and after banding; and the ultrastructure and function of heterochromatin and euchromatin.

Table of Contents

List of Contributors


Contents of Other Volumes


Part I Nuclear Structure

Chapter 1 Localization of Nuclear Functions as Revealed by Ultrastructural Autoradiography and Cytochemistry

I. Localization of Chromatin

II. Sites of Replication

III. Localization of Ribonucleoproteins

IV. Sites of Transcription

V. Nuclear Proteins

VI. Discussion


Chapter 2 The Nucleolus and Nucleolar DNA

I. Historical

II. Variations in Nucleolar Structure

III. The Role of the Nucleolus

IV. The Ultrastructure of the Nucleolus

V. Perinucleolar and Intranucleolar Chromatin Structures

VI. Compact Nucleoli

VII. Nucleoli with Nucleolonemas

VIII. Ring-Shaped Nucleoli

IX. Nucleolar Fibrillar and Granular Components

X. Dense Intranucleolar Particles

XI. The Nucleolar Microspherules

XII. The Nucleolar Light Areas: Interstices and Vacuoles

XIII. Nucleolar Fibrillar Centers and Nucleolini

XIV. Natural Nucleolar Segregation

XV. Nucleolar Constituents

XVI. Synthesis and Cell Content of rDNA

XVII. Summary


Chapter 3 Ultrastructural Organization of Plant Cell Nuclei

I. Introduction

II. Types of Interphase Nuclei

III. Organization and Possible Role of Pufflike Chromosome Segments

IV. Possible Factors Involved in Nuclear Structure Diversity

V. Pattern of Organization of Plant Nucleoli


Chapter 4 The Nucleus of the Plasmodial Slime Molds

I. Introduction

II. Culture Methods and Gross Morphology

III. Microscopic Morphology

IV. Chromosome Numbers

V. Isolation of Nuclei and Nucleoli

VI. Biochemistry


Chapter 5 Structures and Functions of the Nuclear Envelope

I. Introduction

II. The Nuclear Envelope as a Means of Intracellular ComPartmentalization

III. Methodological Progress

IV. The Nuclear Envelope as a Part of the Endoplasmic Reticulum

V. Associations of Inner Nuclear Membrane with Chromosomes and Chromatin

VI. Association of the Inner Nuclear Membrane with Specific Proteinaceous Layers

VII. The Pore Complex

VIII. Relationship of the Nuclear Envelope to Annulate Lamellae (AL) and Intranuclear Cisternae

IX. Structural Differentiation of the Nuclear Envelope

X. Associations and Interactions of the Nuclear Envelope with Microtubules and Microfilaments

XI. Relationship of the Nuclear Envelope to Other Cell Organelles

XII. Breakdowns and Reconstitutions of the Nuclear Envelope; Role of the Nuclear Envelope in Nuclear Divisions and Fusions

Note Added in Proof


Chapter 6 Chemical and Biochemical Properties of the Nuclear Envelope

I. Introduction

II. Isolation and Physical Properties

III. Chemical Properties

IV. Biochemical Properties

V. Conclusions

Note Added in Proof


Part II Nuclear-Cytoplasmic Interactions

Chapter 7 Movement of Molecules between Nucleus and Cytoplasm

I. Introduction

II. Observations from Which It Can Be Inferred That Molecules Move Nonrandomly between Cytoplasm and Nucleus

III. Unidirectional Movement of RNA from Nucleus to Cytoplasm

IV. Unidirectional Movement of Proteins from Cytoplasm to Nucleus

V. Movement of DNA from Nucleus to Cytoplasm

VI. Some Proteins Shuttle Nonrandomly between Nucleus and Cytoplasm

VII. RNA's That Shuttle Nonrandomly between Nucleus and Cytoplasm

VIII. Movement of Ions between Nucleus and Cytoplasm

IX. Conclusions


Chapter 8 Heterokaryons and Their Uses in Studies of Nuclear Function

I. Introduction

II. Methods of Inducing Cell Fusion

III. Mechanism of Cell Fusion

IV. Nuclear Reactivation

V. Gene Expression

VI. Heterokaryons in Virological Studies

VII. Studies on Induction of DNA Synthesis, Mitosis, and Premature Chromosome Condensation

VIII. Conclusion


Chapter 9 The Genome in Specialized Cells, as Revealed by Nuclear Transplantation in Amphibia

I. Introduction

II. Methods of Nuclear Transplantation

III. Evidence for Genetic Identity of Transplanted Somatic Nuclei

IV. Loss of Developmental Capacity of Transplanted Nuclei

V. Conclusions on the Developmental Capacity of Transplanted Nuclei

VI. General Conclusions


Part III Chromatin

Chapter 10 The Molecular Organization of the Chromatin Fiber

I. Introduction

II. Morphological Methods for the Study of Chromatin Fibers

III. Organization of Chromatin Fibers in Thin Sections

IV. Organization of Chromatin Fibers Spread on Liquid Surfaces

V. Composition of Spread Chromatin Fibers

VI. Morphology of the DNA Moiety of Chromatin Fibers

VII. Physical Studies on Nucleohistone and Chromatin

VIII. Packing and Coiling of DNA: Models of the Chromatin Fiber


Chapter 11 The Structure of Human Chromosomes

I. Chromosome Structure before Banding

II. A Prebanding Model

III. Chromosome Structure after Banding

IV. Centromeric versus Intercalary Heterochromatin

V. Mechanisms of Banding

VI. Chromosome Structure after Banding


Chapter 12 Ultrastructure and Function of Heterochromatin and Euchromatin

I. History of Heterochromatin and Euchromatin

II. Ultrastructure of Heterochromatin and Euchromatin

III. Function of Heterochromatin and Euchromatin

IV. Summary


Author Index

Subject Index


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© Academic Press 1974
Academic Press
eBook ISBN:

About the Editor

Harris Busch

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