The Biology of Amoeba

The Biology of Amoeba

1st Edition - January 28, 1973

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  • Editor: Kwang Jeon
  • eBook ISBN: 9780323144049

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Description

The Biology of Amoeba discusses the general biology, morphology, movement and related phenomena, and biochemical and physiological studies of amoeba. This book is organized into five parts, encompassing 21 chapters that primarily focus on large free-living amoeba. After briefly discussing the highlights of studies involving amoeba, the book goes on describing the biological aspects of amoeba, including its taxonomy, phylogeny, culture, and maintaining methods. The second part describes the general morphology, ultrastructure, and cellular membrane of amoeba. The third part includes discussions on the movement of Chaos-Amoeba group; the amoeboid behavioral and motile responses; the molecular mechanism of amoeboid movement and cytoplasmic streaming; and the mechanism of endocytosis in the freshwater amoeba. Part 4 covers the effects of various groups of mutagens, antibiotics, radiation, and high pressure on phenotype change and cell activities of amoeba. The concluding part deals with the isolation and purification of amoeba's nucleic acids, as well as physical and chemical characterizations of these compounds. This part also describes the characteristics of structural features of amoeba's cell surface and the chemistry of tripartite surface. Discussions on cell cycle, nucleocytoplasmic interactions, nuclear-nuclear interactions, genetics, and strain specificity in amoeba are also covered. The book is intended as a comprehensive literature source for students in cell biology as well as for those who are using amoeba as research organisms.

Table of Contents


  • List of Contributors

    Foreword

    Preface

    Part I. General Biology

    Chapter 1. Some Historical Aspects of Amoeba Studies

    I. Introduction

    II. From Observation to Experimentation

    III. The Role of the Interphase Nucleus in Cell Behavior

    IV. Concluding Remarks

    References

    Chapter 2. Taxonomy and Phylogeny

    I. Introductory Remarks

    II. Problems in Taxonomy and Phylogeny

    III. Gross Morphological Distinction of Amoebae

    IV. Taxonomic Schemes for Higher Sarcodine Taxa

    V. Taxonomic Groupings of Lobose Amoebae

    VI. Fine Structure and Taxonomic Distinction

    VII. Ecological Bases for Distinction

    VIII. Physiological Differences

    IX. Biochemical Differences

    X. Mutants

    XI. Hybrids

    XII. A Taxonomic Summary of Data

    XIII. Phylogeny of Large Free-Living Amoebae

    References

    Chapter 3. Culture: Maintenance, Large Yields, and Problems of Approaching Axenic Culture

    I. Introduction

    II. Culture Methods

    III. Discussion

    IV. Concluding Remarks

    References

    Part II. Morphology

    Chapter 4. General Morphology

    I. Introduction

    II. Morphology of Representative Amoebae

    III. Other Large Freshwater Amoebae

    References

    Chapter 5. Ultrastructure

    I. Introduction

    II. Pelomyxa palustris Greeff

    III. Pelomyxa carolinensis Wilson

    IV. Pelomyxa illinoisensis Kudo

    V. Amoeba proteus (Pallas)

    References

    Chapter 6. Cellular Membranes of Amoebae

    I. Introduction

    II. Fine Structure, Cytochemistry, and Functions

    III. Variations with Physiological State

    IV. Origins and Relationships of Membranous Organelles

    V. Concluding Remarks

    References

    Part III. Movement and Related Phenomena

    Chapter 7. Biophysical Aspects of Pseudopodium Formation and Retraction

    I. Introduction

    II. Characteristics of Lobopodial Amoebae

    III. Pseudopodia and Cytoplasmic Streaming

    IV. Streaming in Isolated Cytoplasm

    V. Models, Hypotheses, and Theories of Amoeboid Movement

    VI. Concluding Remarks

    References

    Chapter 8. Locomotion and Behavior

    I. Introduction

    II. The Physicochemical Mechanism of Amoeboid Motion

    III. Nonlocomotive Movements

    IV. Responses of Amoebae to Stimuli

    V. Interactions of Stimuli

    VI. Role of Cell Surface in Behavior

    VII. Nuclear Control of Amoeboid Movement and Behavior

    VIII. Summary

    References

    Chapter 9. Progress in Understanding Amoeboid Movement at the Molecular Level

    I. Introduction

    II. The Mechanism of Force Generation

    III. The Mechanism Controlling Amoeboid Movement

    IV. The Mechanism for Maintaining Regional Variations in Consistency within the Cell

    V. Concluding Remarks

    References

    Chapter 10. Endocytic Processes

    I. Introduction

    II. The Morphology of Endocytosis

    III. The Physiology of Endocytosis

    IV. Enzymes in Relation to Endocytosis

    V. Factors Affecting the Rate of Endocytosis

    VI. Concluding Remarks

    References

    Part IV. Effect of Chemical and Physical Agents

    Chapter 11. Chemical Mutagenesis

    I. Introduction

    II. Classification of Changes Observed in Amoebae

    III. Mutagens

    IV. Mutant Strains of A. proteus Induced by Methyl Nitrosourethane

    V. Studies on the Initial Damage by N-Methyl-TV-nitrosourethane (MNU) Correlated with Cell Cycle Activity

    VI. Induction of Mutations

    VII. Conclusion

    References

    Chapter 12. The Response of Amoebae to Antibiotics

    I. Introduction

    II. Methods of Assay

    III. Antibiotics with Known Sites of Action

    IV. Inhibitors with Less Well-Known Sites of Action

    V. Concluding Remarks

    References

    Chapter 13. Radiation Studies

    I. Introduction

    II. Ionizing Radiations

    III. Ultraviolet Radiation

    IV. β-Irradiation of Amoeba DNA

    V. The Repair of Damage from Radiations

    VI. Conclusion

    References

    Chapter 14. High-Pressure Studies on Amoeba

    I. Introduction

    II. Morphological Studies

    III. Physiological Studies

    IV. Chemical Studies

    V. Concluding Remarks

    References

    Part V. Biochemical and Physiological Studies

    Chapter 15. Nucleic Acids of Amoebae

    I. Introduction

    II. Isolation of the Nucleic Acids

    III. Analysis of the Nucleic Acids

    IV. Conclusions

    References

    Chapter 16. The Chemistry of Amoeba Surface

    I. Introduction

    II. Methods of Analysis

    III. Analysis by Light Microscopy

    IV. Analysis by Electron Microscopy

    V. Chemical Analysis of Subcellular Fractions

    VI. Epilogue

    References

    Chapter 17. The Cell Cycle in Amoebae

    I. Introduction

    II. The Subsections of the Cell Cycle

    III. Attempts to Induce a G1 Period in A. proteus

    IV. How Is the Cell Cycle Arrested when Amoebae Cease Proliferation?

    V. Nucleocytoplasmic Interactions in the Control of DNA Synthesis

    VI. Some Observations on the G2 Period

    VII. Concluding Remarks

    References

    Chapter 18. Nucleocytoplasmic Interactions in Amoebae

    I. Introduction

    II. Nucleocytoplasmic Interactions in Cell Heredity

    III. Nucleocytoplasmic Interactions in the Control of RNA Synthesis

    IV. Nucleocytoplasmic Interactions in the Control of Protein Synthesis

    V. Nucleocytoplasmic Interactions in the Control of DNA Synthesis and the Cell Growth Cycle

    VI. The Interdependence of Nucleus and Cytoplasm

    VII. The Movement of Macromolecules between Nucleus and Cytoplasm

    VIII. Nucleocytoplasmic Interactions in Ameboid Movement

    IX. Conclusion

    References

    Chapter 19. Nuclear-Nuclear Interactions in Heterokaryons

    I. Introduction

    II. Methods of Obtaining Heterokaryons and Types of Possible Heterokaryons

    III. Nuclear-Nuclear Interactions in Nonviable Heterokaryons

    IV. Nuclear-Nuclear Interactions in the Determination of the Heterokaryon Phenotype

    V. Genetic Nuclear-Nuclear Interactions in Heterokaryons

    VI. Concluding Remarks

    References

    Chapter 20. Genetic Information in the Cytoplasm of Amoebae

    I. Early Experiments

    II. Selection of Reliable Characters

    III. Transfer of Information by Injection of Cytoplasm

    IV. Nature of the Molecules Involved—DNA versus RNA

    V, Discussion

    References

    Chapter 21. Strain Specificity in Amoeba proteus

    I. Introduction

    II. Amoebae

    III. Strain-Specific Phenotypic Characters

    IV. Compatibility at the Subcellular Level

    V. Variations in Phenotypic Characters

    VI. Concluding Remarks

    References

    Appendix

    I. Selected References to Methods Used in Amoeba Studies

    II. Some Statistics on Amoebae

    References

    Author Index

    Subject Index




Product details

  • No. of pages: 646
  • Language: English
  • Copyright: © Academic Press 1973
  • Published: January 28, 1973
  • Imprint: Academic Press
  • eBook ISBN: 9780323144049

About the Editor

Kwang Jeon

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