- Contains both theoretical and practical issues in 30 chapters contributed by the world authorities on Tetrahymena
- Indispensible for both the novice and the experienced researcher
- Overviews the history, cell biology, and genetics of the organism
- Describes essential protocols on the growth of cells, genetic techniques, and how to look at the cells with the microscope
- Illustrates how the methods can be applied to solve various cell biological problems
- Reviews recently developed strategies for altering gene expression
Table of Contents
D.L. Nanney and E.M. Simon, Laboratory and Evolutionary History of Tetrahymena thermophila.
J. Frankel, Cell Biology of Tetrahymena thermophila.
K.M. Karrer,Tetrahymena Genetics: Two Nuclei are Better Than One.
E. Orias, E.P. Hamilton, and J.D. Orias, Tetrahymena as a Laboratory Organism: Useful Strains, Cell Culture, and Cell Line Maintenance.
P.J. Bruns, H.R. Smith, and D. Cassidy-Hanley, Long-term Storage.
E.P. Hamilton and E. Orias, Genetic Crosses: Setting up Crosses, Testing Progeny, and Isolating Phenotypic Assortants.
P.J. Bruns and D. Cassidy-Hanley, Methods for Genetic Analysis.
S.L. Allen, Isolation of Micronuclear and Macronuclear DNA.
E. Orias and E.P. Hamilton, Genetically Sorting a Collection of Tetrahymena Mutants.
E.P. Hamilton and E. Orias, Genetically Mapping New Mutants and Cloned Genes.
D.G. Pennock, Selection of Motility Mutants.
K.R. Stuart and E.S. Cole, Nuclear and Cytoskeletal Fluorescence Microscopy Techniques.
E.S. Cole and K.R. Stuart, Nuclear and Cortical Histology for Brightfield Microscopy.
W. Dentler, Fixation of Tetrahymena Cells for Electron Microscopy.
R.H. Gavin, J.G. Hoey, and J.A. Garcés, Immunoelectron Microscopy of Tetrahymena.
A.P. Turkewitz, N.D. Chilcoat, A. Haddad, and J.W. Verbsky, Regulated Protein Secretion in Tetrahymena thermophila.
T.M. Hennessey and H.G. Kuruvilla, Electrophysiology of Tetrahymena.
E.A. Wiley, C.A. Mizzen, and C.D. Allis, Isolation and Characterization of in Vivo Modified Histones and an Activity Gel Assay for Identification of Histone Acetyltransferase.
C. Autexier, Tetrahymena Telomerase Activity, Purification, and Reconstruction.
E. Blackburn, D. Gilley, T. Ware, A. Bhattacharyya, K. Kirk, and H. Wang, Studying the Telomerase RNA in Tetrahymena.
T.M. Gibson and D.J. Asai, Isolation and Characterization of 22S Outer Arm Dynein from Tetrahymena Cilia.
N.E. Williams Preparation of Cytoskeletal Fractions from Tetrahymena thermophila.
N.E. Williams, Immunoprecipitation Procedures.
O. Numato, K. Hanyu, T. Takeda, and Y. Watanabe, Tetrahymena Calcium-Binding Proteins, TCBP-25 and TCBP-23.
D.L. Chalker, J.G. Ward, C. Randolph, and M.-C. Yao, Microinjection of Tetrahymena thermophila.
J. Gaertig and G. Kapler, Transient and Stable DNA Transformation of Tetrahymena thermophila by Electroporation.
P.J. Bruns and D. Cassidy-Hanley, Biolistic Transformation of Macro- and Micronuclei.
B. Hai, J. Gaertig, an M.A. Gorovsky, Knockout Heterokaryons Enable Facile Mutagenic Analysis of Essential Genes in Tetrahymena.
Q. Fan, R. Sweeney, and M.-C. Yao, Creation and Use of Antisense Ribosomes in Tetrahymena thermophila.
L. Yu and M.A. Gorovsky, Protein Tagging in Tetrahymena.
Appendix I: Genetic Nomenclature Rules for Tetrahymena thermophila.
Appendix II: Codon Usage in Tetrahymena thermophila.
Volumes in Series.
- No. of pages: 588
- Language: English
- Copyright: © Academic Press 1999
- Published: August 23, 1999
- Imprint: Academic Press
- eBook ISBN: 9780080524955