Techniques in Confocal Microscopy - 1st Edition - ISBN: 9780123846587, 9780123846594

Techniques in Confocal Microscopy

1st Edition

Editors: P. Michael Conn
Paperback ISBN: 9780123846587
eBook ISBN: 9780123846594
Imprint: Academic Press
Published Date: 14th June 2010
Page Count: 544
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Table of Contents

 

Part I. Practical Considerations and Equipment


1. Theoretical Basis of Confocal Microscopy
Robert H. Webb

2. Practical Considerations in Acquiring Biological Signals from Confocal Microscope
Hao He, Rose Chik Ying Ong, Kam Tai Chan, Ho Pui Ho, Siu Kai Kong

3. Equipment for Mass Storage and Processing of Data
Guy Cox

4. Antifading Agents for Confocal Fluorescence Microscopy
Miguel Berrios, Kimberly A. Conlon, and David E. Colflesh

5. Mounting Techniques for Confocal Microscopy
Manabu Kagayama and Yasuyuki Sasano

6. Preparation of Whole Mounts and Thick Sections for Confocal Microscopy
Hisashi Hashimoto, Hiroshi Ishikawa, and Moriaki Kusakabe
 
7. Use of Confocal Microscopy to Investigate Cell Structure and Function
Ghassan Bkaily, Danielle Jacques

8. Combining Laser Scanning Confocal Microscopy and Electron Microscopy
Xuejun Sun


Part II. Functional Approaches

9. Volume Measurements in Confocal Microscopy
Carlos B. Mantilla, Y.S. Prakash and Gary C. Sieck

10. Quantitation of Phagocytosis by Confocal Microscopy
George F. Babcock and Chad T Robinson MS

11. Receptor-Ligand Internalization
Guido Orlandini, Nicoletta Ronda, Rita Gatti, Gian Carlo Gazzola, and Alberico Borghetti

12. Quantitative Imaging of Metabolism by Two-Photon Excitation Microscopy
David W. Piston and Susan M. Knobel

13. Trafficking of the Androgen Receptor
Virginie Georget, Béatrice Terouanne, Jean-Claude Nicolas, and Charles Sultan

14. Localization of Proteases and Peptide Receptors by Confocal Microscopy
Kanti D. Bhoola, Celia J. Snyman and Carlos D. Figueroa

15. Multiphoton Excitation Microscopy, Confocal Microscopy, and Spectroscopy of Living Cells and Tissues: Functional Metabolic Imaging of Human Skin in Vivo
Barry R. Masters, Peter T. C. So, K


Description

As part of the Reliable Lab Solutions series, Techniques in Confocal Microscopy brings together chapters from volumes 302, 307 and 356 of Methods in Enzymology. It documents many diverse uses for confocal microscopy in disciplines that broadly span biology.

Key Features

  • Documents many diverse uses for confocal microscopy in disciplines that broadly span biology
  • The methods presented include shortcuts and conveniences not included in the initial publications 
  • Techniques are described in a context that allows comparisons to other related methodologies
  • Methodologies are laid out in a manner that stresses their general applicability and reports their potential limitations

Readership

Researchers in and students of biochemistry, molecular and cell biology, developmental biology, and genetics.


Details

No. of pages:
544
Language:
English
Copyright:
© Academic Press 2010
Published:
Imprint:
Academic Press
eBook ISBN:
9780123846594
Paperback ISBN:
9780123846587

About the Editors

P. Michael Conn Editor

P. Michael Conn is the Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center. He is The Robert C. Kimbrough, Professor of Internal Medicine and Cell Biology/Biochemistry. He was previously Director of Research Advocacy and Professor of Physiology and Pharmacology, Cell Biology and Development and Obstetrics and Gynecology at Oregon Health and Science University and Senior Scientist of the Oregon National Primate Research Center (ONPRC). He served for twelve years as Special Assistant to the President and Associate Director of the ONPRC. After receiving a B.S. degree and teaching certification from the University of Michigan (1971), a M.S. from North Carolina State University (1973), and a Ph.D. degree from Baylor College of Medicine (1976), Conn did a fellowship at the NIH, then joined the faculty in the Department of Pharmacology, Duke University Medical Center where he was promoted to Associate Professor in 1982. In 1984, he became Professor and Head of Pharmacology at the University of Iowa College of Medicine, a position he held for eleven years. Conn is known for his research in the area of the cellular and molecular basis of action of gonadotropin releasing hormone action in the pituitary and therapeutic approaches that restore misfolded proteins to function. His work has led to drugs that have benefitted humans and animals. Most recently, he has identified a new class of drugs, pharmacoperones, which act by regulating the intracellular trafficking of receptors, enzymes and ion channels. He has authored or co-authored over 350 publications in this area and written or edited over 200 books, including texts in neurosciences, molecular biology and endocrinology. Conn has served as the editor of many professional journals and book series (Endocrinology, Journal of Clinical Endocrinology and Metabolism, Endocrine, Methods, Progress in Molecular Biology and Translational Science and Contemporary Endocrinology). Conn serve

Affiliations and Expertise

Texas Tech University Health Sciences Center, Lubbock, USA