As part of the Reliable Lab Solutions series, Techniques in Confocal Microscopy brings together chapters from volumes 302, 307 and 356 of Methods in Enzymology. It documents many diverse uses for confocal microscopy in disciplines that broadly span biology.

Key Features

  • Documents many diverse uses for confocal microscopy in disciplines that broadly span biology
  • The methods presented include shortcuts and conveniences not included in the initial publications 
  • Techniques are described in a context that allows comparisons to other related methodologies
  • Methodologies are laid out in a manner that stresses their general applicability and reports their potential limitations


Researchers in and students of biochemistry, molecular and cell biology, developmental biology, and genetics.

Table of Contents


Part I. Practical Considerations and Equipment

1. Theoretical Basis of Confocal Microscopy
Robert H. Webb

2. Practical Considerations in Acquiring Biological Signals from Confocal Microscope
Hao He, Rose Chik Ying Ong, Kam Tai Chan, Ho Pui Ho, Siu Kai Kong

3. Equipment for Mass Storage and Processing of Data
Guy Cox

4. Antifading Agents for Confocal Fluorescence Microscopy
Miguel Berrios, Kimberly A. Conlon, and David E. Colflesh

5. Mounting Techniques for Confocal Microscopy
Manabu Kagayama and Yasuyuki Sasano

6. Preparation of Whole Mounts and Thick Sections for Confocal Microscopy
Hisashi Hashimoto, Hiroshi Ishikawa, and Moriaki Kusakabe
7. Use of Confocal Microscopy to Investigate Cell Structure and Function
Ghassan Bkaily, Danielle Jacques

8. Combining Laser Scanning Confocal Microscopy and Electron Microscopy
Xuejun Sun

Part II. Functional Approaches

9. Volume Measurements in Confocal Microscopy
Carlos B. Mantilla, Y.S. Prakash and Gary C. Sieck

10. Quantitation of Phagocytosis by Confocal Microscopy
George F. Babcock and Chad T Robinson MS

11. Receptor-Ligand Internalization
Guido Orlandini, Nicoletta Ronda, Rita Gatti, Gian Carlo Gazzola, and Alberico Borghetti

12. Quantitative Imaging of Metabolism by Two-Photon Excitation Microscopy
David W. Piston and Susan M. Knobel

13. Trafficking of the Androgen Receptor
Virginie Georget, Béatrice Terouanne, Jean-Claude Nicolas, and Charles Sultan

14. Localization of Proteases and Peptide Receptors by Confocal Microscopy
Kanti D. Bhoola, Celia J. Snyman and Carlos D. Figueroa

15. Multiphoton Excitation Microscopy, Confocal Microscopy, and Spectroscopy of Living Cells and Tissues: Functional Metabolic Imaging of Human Skin in Vivo
Barry R. Masters, Peter T. C. So, K


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© 2010
Academic Press
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