Spin Labeling

Theory and Applications

1st Edition - April 28, 1979
Editor: Lawrence J. Berliner
Language: English
eBook ISBN:
9 7 8 - 1 - 4 8 3 2 - 1 6 4 1 - 6

Spin Labeling II: Theory and Applications covers spin-labeled biopolymers and its importance to both macromolecular modeling as well as some industrially based problems. The book… Read more

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Spin Labeling II: Theory and Applications covers spin-labeled biopolymers and its importance to both macromolecular modeling as well as some industrially based problems. The book discusses the methods of saturation-transfer spectroscopy; the spin-probe-spin-label method; and new aspects of nitroxide chemistry. The text also describes the spin-labeled synthetic polymers; the impact that the technique of spin labeling has on pharmacology and on biomedicine; as well as the applications of spin labeling to nucleic acids. Chemists, pharmacologists, biochemists, and people working in the field of biological magnetic resonance will find the book invaluable.

List of Contributors

Preface

1. Saturation-Transfer Spectroscopy

I. Introduction

II. Physics of the Very Slow Tumbling Domain

III. Experimental Methods

IV. Theory

V. Computational Efficiency

VI. Applications of Saturation-Transfer Electron Paramagnetic Resonance

VII. Anisotropic or Special Motions

References

2. The Spin-Probe-Spin-Label Method

I. Introduction

II. Theory

III. Survey of Chemical and Magnetic Properties of Paramagnetic Metal-Ion Probes

IV. Applications of the Method

References

3. New Aspects of Nitroxide Chemistry

I. Introduction

II. Structural Features That Make a Nitroxide Stable

III. Characterization of New Nitroxide Spin Labels and Nitroxide Spectra

IV. Synthesis of New Stable Nitroxide Spin Labels

V. Chemical Reactions Involving the Nitroxide Grouping

VI. Chemical Reactions Not Involving the Nitroxide Grouping

VII. Dinitroxide Spin Labels

VIII. Experimental Procedures for the Synthesis of Selected Proxyl and Imidazolidine-Derived Nitroxide Spin Labels

References

4. Spin-Labeled Synthetic Polymers

I. Introduction

II. Sample Preparation

III. Analysis in Polymeric Materials

IV. Motion in Bulk Polymers

V. Effect of Diluents

VI. Conformation of Polymers Adsorbed at Solid-Liquid Interfaces

VII. Conclusion

References

5. Spin Labeling in Pharmacology

I. Introduction

II. Drug Absorption, Distribution, and Excretion

III. Drug Metabolism

IV. Pharmacological Effects of Drugs

V. Future of Spin-Labeling Studies in Pharmacology

References

6. Spin Labeling in Biomedicine

I. Introduction

II. Spin Assay

III. Spin Immunoassay

IV. Spin-Membrane Immunoassay

V. Spin Labeling in Carcinogenesis

VI. Spin Trapping

VII. In Vivo Electron Spin Resonance

References

7. Applications of Spin Labeling to Nucleic Acids

I. Introduction

II. Spin Labeling-Spin Probing of Nucleic Acids

III. Electron Spin Resonance Spectra of Nucleic Acids Containing Spin Labels or Spin Probes

IV. Monitoring Conformational Changes in Nucleic Acids by Electron Spin Resonance

V. Molecular Association Studies with Spin-Labeled Nucleic Acids

VI. Conclusion

References

Appendix I

Appendix II Updated List of Commercial Sources of Spin Labels and Nitroxide Precursors

Index