Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. This volume captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools.

Key Features

    * Includes time-tested core methods and new innovations applicable to any researcher employing single molecule tools
    * Methods included are useful to both established researchers and newcomers to the field
    * Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines


    Biochemists, geneticists, molecular biologists, cell biologists, and biophysicists.

    Table of Contents

    1. Watching single DNA replication loops under flow extension
    2. Star polymer surface passivation for single molecule detection
    3. Ultrahigh resolution detection of single active motor proteins in live cells
    4. Molecules and Methods for Superresolution Imaging
    5. Aqueous nanodroplets for studying single molecules
    6. High-speed atomic force microscopy techniques for visualizing dynamic behavior of biological macromolecules
    7. Single-Biomolecule Spectroscopy Using Microfluidic Platforms
    8. DNA Looping Kinetics Analyzed by Tethered Particle Microscopy
    9. Single molecule observation of proteins in vivo
    10. DNA curtains as a high-throughput approach to single molecule imaging
    11. Single-molecule enzymology of protein synthesis
    12. Single molecule fluorescence studies of intrinsically disordered proteins
    13. Nanovesicle trapping for studying transient protein-protein interactions by single molecule FRET
    13. Tracking single motor proteins in the cytoplasm of mammalian cells
    14. Conformational States of F1-ATPase by Single-Molecule Rotation
    15. Single Molecule Sequencing by Fluorescence Imaging
    16. Real-Time DNA Sequencing from Single Polymerase Molecules
    17. Micropatterning and single molecule imaging for quantitative analysis of protein-protein interactions in living cells
    18. Probing virus-receptor interactions by atomic force spectroscopy
    19. Single-Molecule Fluorescence Spectroscopy of Cytochrome P450 in Nanodiscs
    20. Analysis of complex single molecule FRET time traces
    21. Application of super-resolution imaging to single particle tracking in nanotechnology
    22. Scanning FCS for the characterization of protein dynamics in live cells
    23. Single mRNA molecule tracking in live cells
    24. Single-molecule high-resolution colocalization (SHREC) or Single-molecule optical-trap analyses of protein structure
    25. Nanopore force Spectroscopy tools for analyzing single bio-complexes
    26. Use of pl


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    © 2010
    Academic Press
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