Separation of Cells and Subcellular Elements

Separation of Cells and Subcellular Elements

Proceedings of a Meeting Organised by EFRAC (European Working Party for the Separation and Detection of Biological Fractions), Sponsored by the Committee for Science and Technology of the Council of Europe, Brussels, 4-5 May 1979

1st Edition - January 1, 1979

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  • Editor: H. Peeters
  • eBook ISBN: 9781483189352

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Description

Separation of Cells and Subcellular Elements presents the chemical engineering approaches in bio-separation methods. This book evaluates the cellular aggregation in turbulent suspension, the phase partition of cells and subcellular particles, and the continuous free-flow electrophoresis. The first chapters deal with flow cytometric characterization of tumor associated changes in gynecologic malignancies; steady state rheolectrolysis; and electrophoretic approaches applicable to cell separation. The succeeding chapters consider the chromosome separation by velocity sedimentation at unit gravity and the separation of human lymphocytes based on volume and density. Other chapters describe the analysis of the gradient former for zonal rotors and the centrifugal elutriation of living cells. The last chapters are devoted to the human platelet isolation from whole blood on metrizamide gradients. The book can provide useful information to biochemists, cytologists, students, and researchers.

Table of Contents


  • Preface

    General Problems

    Chemical Engineering Approaches in Bio-separation Methods

    Cellular Aggregation in Turbulent Suspensions

    Flow Cytometry

    Flow Cytometric Characterization of Tumor Associated

    Changes in Gynecologic Malignancies

    Phase Partition

    Phase Partition of Cells and Subcellular Particles

    Electrophoresis

    Continuous Free-flow Electrophoresis

    New Electrophoretic Approaches Applicable to Cell Separation

    Steady State Rheolectrolysis. II. Experimental Endeavor

    Unit Gravity Sedimentation

    A Sorter of Cells, Chromosomes and Nuclei that Combines

    Simplicity with Good Resolution

    Chromosome Separation by Velocity Sedimentation at Unit Gravity

    Utracentrifugal Methods

    Elutriation

    Centrifugal Elutriation: Separation of Living Cells

    Density Gradients

    A New Gradient Former for Zonal Rotors

    The Use of Density Gradients of Percoll® for the Separation of Biological Particles

    Improved Separation of Human Peripheral T-cells Using

    PVP-coated Colloidal Silica Particles (Percoll)

    Separation of Human Lymphocytes on the Basis of Volume and Density

    Human Platelet Isolation from Whole Blood on Metrizamide Gradients

    Isolation of Dense Bodies from Human Blood Platelets using Discontinuous Metrizamide Gradients

    Exchange of Cholesterol Between the Subcellular Fractions

    During Differential Centrifugation of Rat Adrenocortical Tissue

    The Isolation of Stable Cattle Rod Outer Segments with an Intact Plasma Membrane

    Continuous Flow

    Studies on Large Scale Preparation of Polymerized Human Red

    Cells Ghosts able to Neutralize Blood Groups Antibodies

    Large-scale Concentration and Purification of Various Animal

    Viruses Using Isopycnic Banding in a Continuous-flow Ultracentrifuge

    Index

Product details

  • No. of pages: 116
  • Language: English
  • Copyright: © Pergamon 1979
  • Published: January 1, 1979
  • Imprint: Pergamon
  • eBook ISBN: 9781483189352

About the Editor

H. Peeters

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