Separation of Cells and Subcellular Elements

Separation of Cells and Subcellular Elements presents the chemical engineering approaches in bio-separation methods. This book evaluates the cellular aggregation in turbulent suspension, the phase partition of cells and subcellular particles, and the continuous free-flow electrophoresis. The first chapters deal with flow cytometric characterization of tumor associated changes in gynecologic malignancies; steady state rheolectrolysis; and electrophoretic approaches applicable to cell separation. The succeeding chapters consider the chromosome separation by velocity sedimentation at unit gravity and the separation of human lymphocytes based on volume and density. Other chapters describe the analysis of the gradient former for zonal rotors and the centrifugal elutriation of living cells. The last chapters are devoted to the human platelet isolation from whole blood on metrizamide gradients.
The book can provide useful information to biochemists, cytologists, students, and researchers.

Preface

General Problems

Chemical Engineering Approaches in Bio-separation Methods

Cellular Aggregation in Turbulent Suspensions

Flow Cytometry

Flow Cytometric Characterization of Tumor Associated

Changes in Gynecologic Malignancies

Phase Partition

Phase Partition of Cells and Subcellular Particles

Electrophoresis

Continuous Free-flow Electrophoresis

New Electrophoretic Approaches Applicable to Cell Separation

Steady State Rheolectrolysis. II. Experimental Endeavor

Unit Gravity Sedimentation

A Sorter of Cells, Chromosomes and Nuclei that Combines

Simplicity with Good Resolution

Chromosome Separation by Velocity Sedimentation at Unit Gravity

Utracentrifugal Methods

Elutriation

Centrifugal Elutriation: Separation of Living Cells

Density Gradients

A New Gradient Former for Zonal Rotors

The Use of Density Gradients of Percoll® for the Separation of Biological Particles

Improved Separation of Human Peripheral T-cells Using

PVP-coated Colloidal Silica Particles (Percoll)

Separation of Human Lymphocytes on the Basis of Volume and Density

Human Platelet Isolation from Whole Blood on Metrizamide Gradients

Isolation of Dense Bodies from Human Blood Platelets using Discontinuous Metrizamide Gradients

Exchange of Cholesterol Between the Subcellular Fractions

During Differential Centrifugation of Rat Adrenocortical Tissue

The Isolation of Stable Cattle Rod Outer Segments with an Intact Plasma Membrane

Continuous Flow

Studies on Large Scale Preparation of Polymerized Human Red

Cells Ghosts able to Neutralize Blood Groups Antibodies

Large-scale Concentration and Purification of Various Animal

Viruses Using Isopycnic Banding in a Continuous-flow Ultracentrifuge

Index