Separation Methods in Organic Chemistry and Biochemistry

Separation Methods in Organic Chemistry and Biochemistry

1st Edition - January 1, 1969

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  • Author: Frank J. Wolf
  • eBook ISBN: 9781483220680

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Separation Methods in Organic Chemistry and Biochemistry aims to provide perspectives for the commonly used separations methods and to discuss indications for their use. The book discusses the determination of molecular properties useful in separation based on micro test methods, paper chromatography, thin-layer chromatography, and electrophoresis. The text then describes the theoretical principles of group-separation procedures, liquid-liquid partition, ion-exchange selectivity, gel permeation, and adsorption. Methods of influencing the selectivity coefficients, the basic theory of fractionation methods, and the principles of application are also encompassed. Biochemists and chemists will find the book useful.

Table of Contents

  • Preface


    I. Types of Separation Considered

    II. Distribution Coefficient

    III. Use of Micro Methods

    IV. Evaluation of Separation Processes

    I. General Principles

    I. Stability

    II. Group Separations

    III. Fractionation Separations


    II. Determination of Molecular Properties

    I. Introduction

    II. Chromatographie Procedures

    III. Micro Tests


    III. Solvent Extraction

    I. Partition Ratio

    II. Application of Solvent Extraction for Group Separation

    III. Application of Solvent Extraction for Fractionation Separation

    IV. Partition Chromatography

    V. Countercurrent Distribution


    IV. Gel Filtration and Gel-Permeation Chromatography

    I. Mechanism

    II. Substances Used for Gel Separations

    III. Column Performance

    IV. Factors Influencing Kd

    V. Applications of Gel Filtration

    VI. Applications of Gel-Permeation Chromatography


    V. Ion Exchange

    I. General Discussion

    II. Ion Exchange Applications


    VI. Adsorption Processes

    I. General Principles

    II. Application of Adsorption Procedures


    Appendix I. Dielectric Constants of Some Common Solvents (at 25°C)

    Appendix II. Ion Exchange Resins

    Appendix III. General Thin-Layer and Paper Chromatographie Techniques

    Appendix IV. Conversion Table for Rf and Rm

    Author Index

    Subject Index

Product details

  • No. of pages: 246
  • Language: English
  • Copyright: © Academic Press 1969
  • Published: January 1, 1969
  • Imprint: Academic Press
  • eBook ISBN: 9781483220680

About the Author

Frank J. Wolf

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