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Selection of the HPLC Method in Chemical Analysis - 1st Edition - ISBN: 9780128036846, 9780128037119

Selection of the HPLC Method in Chemical Analysis

1st Edition

Authors: Serban Moldoveanu Victor David
Paperback ISBN: 9780128036846
eBook ISBN: 9780128037119
Imprint: Elsevier
Published Date: 1st November 2016
Page Count: 598
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Selection of the HPLC Method in Chemical Analysis serves as a practical guide to users of high-performance liquid chromatography and provides criteria for method selection, development, and validation.

High-performance liquid chromatography (HPLC) is the most common analytical technique currently practiced in chemistry. However, the process of finding the appropriate information for a particular analytical project requires significant effort and pre-existent knowledge in the field. Further, sorting through the wealth of published data and literature takes both time and effort away from the critical aspects of HPLC method selection.

For the first time, a systematic approach for sorting through the available information and reviewing critically the up-to-date progress in HPLC for selecting a specific analysis is available in a single book. Selection of the HPLC Method in Chemical Analysis is an inclusive go-to reference for HPLC method selection, development, and validation.

Key Features

  • Addresses the various aspects of practice and instrumentation needed to obtain reliable HPLC analysis results
  • Leads researchers to the best choice of an HPLC method from the overabundance of information existent in the field
  • Provides criteria for HPLC method selection, development, and validation
  • Authored by world-renowned HPLC experts who have more than 60 years of combined experience in the field


Analytical chemists practicing HPLC and students at the graduate level taking related coursework in analytical chemistry

Table of Contents

  • Preface
  • Chapter 1. Start of the Implementation of a New HPLC Method
    • 1.1. Collection of Information and Planning for a New Method
    • 1.2. Overview of an Analytical Technique
    • 1.3. Statistical Evaluation of Data and Criteria for Method Validation
  • Chapter 2. Short Overviews of Analytical Techniques Not Containing an Independent Separation Step
    • 2.1. Summary Classification of Analytical Techniques Not Containing an Independent Separation
    • 2.2. Optical Techniques
    • 2.3. Mass Spectrometry
    • 2.4. Electrochemical Methods
    • 2.5. Other Analytical Techniques Not Including the Separation of Sample Components
    • 2.6. Selection of a Nonhyphenated Method of Analysis versus one Containing a Separation Step
  • Chapter 3. Short Overviews of the Main Analytical Techniques Containing a Separation Step
    • 3.1. Separation Types Used in the Core Analytical Techniques
    • 3.2. Gas Chromatography
    • 3.3. Supercritical Fluid Chromatography
    • 3.4. High-Performance Liquid Chromatography
    • 3.5. Electrophoresis and Electrochromatography
    • 3.6. Selection of GC, SFC, CZE, or HPLC
  • Chapter 4. Basic Information Regarding the HPLC Techniques
    • 4.1. Basic Information About Instrumentation in HPLC
    • 4.2. Parameters Describing the Chromatographic Process
    • 4.3. Retention and Elution Mechanisms in Different Types of HPLC
    • 4.4. The Influence of pH, Temperature, and Additives on Retention Equilibria
  • Chapter 5. Properties of Analytes and Matrices Determining HPLC Selection
    • 5.1. Physicochemical Properties Related to Separation
    • 5.2. Physicochemical Properties Related to Detection
    • 5.3. Selection of the HPLC Separation Based on Sample Properties
    • 5.4. Selection of the HPLC Separation Based on Analyte Properties
    • 5.5. Selection of the HPLC Separation Depending on the Matrix
    • 5.6. Review of Sample Properties with the Goal of Selection of a Detector in HPLC
  • Chapter 6. General Aspects Regarding the HPLC Analytical Column
    • 6.1. Construction of the High-Performance Liquid Chromatography (HPLC) Column
    • 6.2. Column Properties Affecting Separation
    • 6.3. General Rules for Column Selection, Utilization, and Care
  • Chapter 7. RP-HPLC Analytical Columns
    • 7.1. Types of Reversed Phases and Their Preparation
    • 7.2. Properties of Reversed-Phase HPLC Columns
    • 7.3. Parameters Used for the Characterization of Reversed-Phase HPLC Columns
    • 7.4. Common and Special RP Columns
    • 7.5. Selection of an RP-HPLC Column
  • Chapter 8. Polar Analytical Columns
    • 8.1. Types of Polar HPLC Phases and Their Preparation
    • 8.2. Properties and Characterization of Polar HPLC Phases
    • 8.3. Columns With a Polar Stationary Phase
    • 8.4. Selection of a Polar Column
  • Chapter 9. Stationary Phases and Columns for Ion Exchange, Ion-Moderated, and Ligand Exchange Chromatography
    • 9.1. Types of Phases and their Preparation
    • 9.2. Characterization of Ion Exchange Phases
    • 9.3. Common Columns with Ion Exchange Phases
    • 9.4. Selection of an Ion Exchange Phase
  • Chapter 10. Stationary Phases and Columns for Chiral Chromatography
    • 10.1. Types of Chiral Phases and Their Preparation
    • 10.2. Characterization of Chiral Phases
    • 10.3. Selection of a Chiral Phase
  • Chapter 11. Stationary Phases and Columns for Size Exclusion
    • 11.1. Types of Phases and Their Preparation
    • 11.2. Characterization of Size-Exclusion Phases and Columns
    • 11.3. Selection of Columns Used in SEC
  • Chapter 12. Stationary Phases and Columns for Immunoaffinity Type Separations
    • 12.1. Types of Phases and Their Preparation
  • Chapter 13. Solvents, Buffers, and Additives Used in the Mobile Phase
    • 13.1. Characterization of Liquids as Solvents
    • 13.2. Additional Properties of Liquids Affecting Separation
    • 13.3. Properties of Solvents of Importance for Detection
    • 13.4. Buffers and Additives
    • 13.5. Selection of Mobile Phase in HPLC
    • 13.6. Selection of a Solvent for Sample Injection
    • 13.7. Selection of a Solvent for the Needle Wash
  • Chapter 14. Gradient Elution
    • 14.1. The Use of Gradient in HPLC
    • 14.2. Parameters Characterizing the Gradient Separation
    • 14.3. Selection of Gradient in Different Chromatographic Types
  • Chapter 15. The Practice of HPLC
    • 15.1. The Development of an HPLC Method
    • 15.2. Special HPLC Techniques
  • Appendix 1. USP Classification of HPLC Columns
  • Appendix 2. Hydrophobic Stationary Phases
  • Appendix 3. HILIC and NPC Stationary Phases
  • Appendix 4. Ion Exchange and Ion-Moderated Stationary Phases
  • Appendix 5. Chiral Stationary Phases
  • Appendix 6. Size-Exclusion Stationary Phases
  • Appendix 7. Properties of Mobile Phase Components
  • Index


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© Elsevier 2016
1st November 2016
Paperback ISBN:
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About the Authors

Serban Moldoveanu

Serban Moldoveanu

Dr. Serban C. Moldoveanu is Senior Principal Scientist at R. J. Reynolds Tobacco Company. His research activity is focused on various aspects of chromatography including method development for the analysis by GC/MS, HPLC, and LC/MS/MS of natural products and cigarette smoke. He has also performed research on pyrolysis of a variety of polymers and small molecules. He has over 100 publications in peer reviewed journals, eleven books, and several chapter contributions. He is a member of the editorial board of the Journal of Analytical Methods in Chemistry.

Affiliations and Expertise

Senior Principal Scientist, RJ Reynolds Tobacco Co., Winston-Salem, NC, USA

Victor David

Victor David

Dr. Victor David is a Professor and Head of the Department of Analytical Chemistry, University of Bucharest, Romania. He is the author of more than 120 publications, including 92 scientific papers in ISI international journals and 10 books and chapters in various Encyclopedias. He is also a reviewer at several international journals.

Affiliations and Expertise

Professor and Head of the Department of Analytical Chemistry, University of Bucharest, Romania

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