Specific complexes of protein and RNA carry out many essential biological functions, including RNA processing, RNA turnover, and RNA folding, as well as the translation of genetic information from mRNA into protein sequences. Messenger RNA (mRNA) decay is now emerging as an important control point and a major contributor to gene expression. Continuing identification of the protein factors and cofactors and mRNA instability elements responsible for mRNA decay allow researchers to build a comprehensive picture of the highly orchestrated processes involved in mRNA decay and its regulation.

Key Features

* Covers the nonsense-mediated mRNA decay (NMD) or mRNA surveillance pathway * Expert researchers introduce the most advanced technologies and techniques * Offers step-by-step lab instructions, including necessary equipment and reagents


Biochemists and researchers in related life science disciplines.

Table of Contents

Methods to Study No-Go Decay in Saccharomyces cerevisiae Cell-Cycle Regulation of Histone mRNA Degradation in Mammalian Cells: Role of Translation and Oligouridylation Assays of ARE-Mediated mRNA Decay in Cells Evaluating the Control of mRNA Decay in Fission Yeast In Vivo Analysis of the Decay of Transcripts Generated by Cytoplasmic RNA Viruses Qualitative and quantitative assessment of the activity of the yeast NMD pathway Nonsence Mediated mRNA Decay in C. elegans In vivo Analysis of Plant Nonsense-Mediated Decay. Estimating Nuclear mRNA Decay in S. Cerevisiae Identification and Analysis of tRNAs that are Degraded in Saccharomyces cerevisiae Due to Lack of Modifications Analysis of Non-Functional rRNA Decay in Saccharomyces cerevisiae Identifying Substrates of mRNA Decay Factors by a Combined RNA Interference and DNA Microarray Approach Analysis of RNA-Protein Interactions Using a Yeast Three-Hybrid System Co-Immunoprecipitation Techniques for Assessing RNA-Protein Interactions in vivo How to Define Targets for Small Guide RNAs in RNA Silencing: A Biochemical Approach Rapid mRNA Deadenylation and Decay Triggered by MicroRNAs A Method to Detect miRNA Targets Using miRNA as a Primer


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© 2008
Academic Press
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About the editors

Lynne E. Maquat

Affiliations and Expertise

University of Rochester, NY, USA

Megerditch Kiledjian

Affiliations and Expertise

Rutgers University, New Brunswick, NJ, USA