RNA Methodologies
A Laboratory Guide for Isolation and Characterization
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RNA Methodologies: A Laboratory Guide for Isolation and Characterization, Sixth Edition provides the most up-to-date ribonucleic acid lab techniques for seasoned scientists and graduate students alike. This edition features new material on RNA sequencing, RNA in Situ Hybridization, non-coding RNAs, computational RNA biology, transcriptomes and bioinformatics, along with the latest advances in methods and protocols across the field of RNA investigation. As a leader in the field, Dr. Farrell provides a wealth of knowledge on the topic of RNA biology while also giving readers helpful hints and troubleshooting techniques from his own personal experience in this subject area. This book presents the essential knowledge and techniques to use when working with RNA for the experienced practitioner, while also aiding the beginner in fully understanding this important branch of molecular biology.
Key Features
- Presents the latest information covering all aspects of working with RNA, delivering a holistic understanding of this leading field in molecular biology
- Builds from basic information on RNA techniques to in-depth protocols for specific applications
- Features new chapters on RNA sequencing and RNA in situ hybridization
- Includes new material on RNA clinical applications and innovations, including RNA therapeutics and RNA vaccines, with particular relevance to coronavirus
- Comprises the latest developments in transcriptomes and bioinformatics, with new material on computational RNA biology, RNA CHiP analysis, aptamer biology and RNA epigenetics
Readership
Researchers and investigators in molecular biology and biochemistry, biomedicine, and related fields. Graduate and postdoctoral students in molecular biology, biomedicine, and related life science programs
Table of Contents
- Preface
Preface to Fourth Edition
Preface to Third Edition
Preface to Second Edition
Preface to First Edition
List of Tables
List of Figures
Acknowledgements
Glossary
1. An introduction to Anaesthesia and Anaesthetic Equipment
2. Anaesthetic agents
3. Monitoring and Managing Anaesthesia
4. Specialised techniques
5. Analgesia and Post-operative care
6. Anaesthesia of Common Laboratory Species
Appendix 1. Physiological data
Appendix 2. Estimation of use of inhalational agents and carrier gases
Appendix 3. Calculations of dilutions of anaesthetic mixtures for small rodents
Appendix 4. Manufacturers of equipment and other items illustrated or cited in the text
Bibliography
Product details
- No. of pages: 960
- Language: English
- Copyright: © Academic Press 2022
- Published: November 22, 2022
- Imprint: Academic Press
- Paperback ISBN: 9780323902212
- eBook ISBN: 9780323916561
About the Author
Robert Farrell, Jr.
Dr. Robert Farrell is a bench-current scientist who has 35 years of experience working with RNA in the study of transcriptional and posttranscriptional regulation of gene expression in a variety of model systems. He is also experienced in animal cell culture methods. Prior to joining the faculty at Penn State University, he operated a biotech education and service firm, winning the 1998 Small Business Contractor of the Year award from the U.S. Department of Agriculture. He is the recipient of campus- and college-wide awards for excellence in teaching, and has extensive experience running RNA and specialized biotechnology hands-on laboratory training programs all over the world. He often serves as a consultant within the pharmaceutical and biotech industries. Dr. Farrell received his Ph.D. and M.S. degrees from The Catholic University of America and his B.S. in Biology from Providence College. Dr. Farrell currently serves as the campus academic officer at Penn State York.
Affiliations and Expertise
Bench-Current Scientist, Pennsylvania State University, York, PA, USA