RNA Methodologies

RNA Methodologies

A Laboratory Guide for Isolation and Characterization

6th Edition - October 1, 2022

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  • Author: Robert Farrell, Jr.
  • Paperback ISBN: 9780323902212

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RNA Methodologies: A Laboratory Guide for Isolation and Characterization, Sixth Edition provides the most up-to-date ribonucleic acid lab techniques for seasoned scientists and graduate students alike. This edition features new material on RNA sequencing, RNA in Situ Hybridization, non-coding RNAs, computational RNA biology, transcriptomes and bioinformatics, along with the latest advances in methods and protocols across the field of RNA investigation. As a leader in the field, Dr. Farrell provides a wealth of knowledge on the topic of RNA biology while also giving readers helpful hints and troubleshooting techniques from his own personal experience in this subject area. This book presents the essential knowledge and techniques to use when working with RNA for the experienced practitioner, while also aiding the beginner in fully understanding this important branch of molecular biology.

Key Features

  • Presents the latest information covering all aspects of working with RNA, delivering a holistic understanding of this leading field in molecular biology
  • Builds from basic information on RNA techniques to in-depth protocols for specific applications
  • Features new chapters on RNA sequencing and RNA in situ hybridization
  • Includes new material on RNA clinical applications and innovations, including RNA therapeutics and RNA vaccines, with particular relevance to coronavirus
  • Comprises the latest developments in transcriptomes and bioinformatics, with new material on computational RNA biology, RNA CHiP analysis, aptamer biology and RNA epigenetics


Researchers and investigators in molecular biology and biochemistry, biomedicine, and related fields. Graduate and postdoctoral students in molecular biology, biomedicine, and related life science programs

Table of Contents

  • 1. RNA and the Cellular Biochemistry Revisited
    2. Creating a Ribonuclease-Free Environment
    3. Stringency: Conditions That Influence Nucleic Acid Structure
    4. RNA Isolation Strategies
    5. Isolation of Polyadenylated RNA
    6. The Truth About Tissues
    7. Going Green: RNA and the Molecular Biology of Plants
    8. Quality Control for RNA Preparations
    9. cDNA: A Permanent Biochemical Record of the Cell
    10. RT-PCR: A Science and an Art Form
    11. Quantitative PCR Techniques
    12. miRNA and Other Noncoding RNAs
    13. RNA Interference and RNA Editing
    14. Electrophoresis of RNA
    15. Photodocumentation and Image Analysis
    16. Northern Analysis
    17. Nucleic Acid Probe Technology
    18. Quantification of Specific mRNAs by Nuclease Protection
    19. Analysis of Nuclear RNA
    20. RNA In-situ Hybridization
    21. Array Analysis of Gene Expression
    22. Nonarray Methods for Global Analysis of Gene Expression
    23. Transcriptomes and Bioinformatics
    24. RNA-seq: The Premier Transcriptomics Tool
    25. RNA Biomarker Discovery and)
    26. Functional Genomics and Transcript Profiling
    27. A Few RNA Success Stories

    A. Maintaining Complete and Accurate Records
    B. Converting Mass to Moles
    C. Removal of DNA from an RNA Sample
    D. Removal of RNA from a DNA Sample
    E. Trypsinization Protocol for Anchorage-Dependent Cells
    F. Electrophoresis: Principles, Parameters, and Safety
    G. Disposal of Ethidium Bromide and SYBR Green Solutions
    H. Polyacrylamide Gel Electrophoresis
    I. Centrifugation as a Mainstream Tool for the Molecular Biologist
    J. Silanizing Centrifuge Tubes and Glassware
    K. Dot Blot Analysis
    L. Deionization of Formamide, Formaldehyde, and Glyoxal
    M. Useful Stock Solutions for the Molecular Biologist
    N. Genomes and Proteomes
    O. Useful SI Units
    P. Common Abbreviations
    Q. Selected Suppliers of Equipment, Reagents, and Services
    R. Trademark Citations

Product details

  • No. of pages: 920
  • Language: English
  • Copyright: © Academic Press 2022
  • Published: October 1, 2022
  • Imprint: Academic Press
  • Paperback ISBN: 9780323902212

About the Author

Robert Farrell, Jr.

Dr. Robert Farrell is a bench-current scientist who has 35 years of experience working with RNA in the study of transcriptional and posttranscriptional regulation of gene expression in a variety of model systems. He is also experienced in animal cell culture methods. Prior to joining the faculty at Penn State University, he operated a biotech education and service firm, winning the 1998 Small Business Contractor of the Year award from the U.S. Department of Agriculture. He is the recipient of campus- and college-wide awards for excellence in teaching, and has extensive experience running RNA and specialized biotechnology hands-on laboratory training programs all over the world. He often serves as a consultant within the pharmaceutical and biotech industries. Dr. Farrell received his Ph.D. and M.S. degrees from The Catholic University of America and his B.S. in Biology from Providence College. Dr. Farrell currently serves as the campus academic officer at Penn State York.

Affiliations and Expertise

Bench-Current Scientist, Pennsylvania State University, York, PA, USA

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