RNA Methodologies

RNA Methodologies

A Laboratory Guide for Isolation and Characterization

6th Edition - November 22, 2022

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  • Author: Robert Farrell, Jr.
  • Paperback ISBN: 9780323902212
  • eBook ISBN: 9780323916561

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RNA Methodologies: A Laboratory Guide for Isolation and Characterization, Sixth Edition provides the most up-to-date ribonucleic acid lab techniques for seasoned scientists and graduate students alike. This edition features new material on RNA sequencing, RNA in Situ Hybridization, non-coding RNAs, computational RNA biology, transcriptomes and bioinformatics, along with the latest advances in methods and protocols across the field of RNA investigation. As a leader in the field, Dr. Farrell provides a wealth of knowledge on the topic of RNA biology while also giving readers helpful hints and troubleshooting techniques from his own personal experience in this subject area. This book presents the essential knowledge and techniques to use when working with RNA for the experienced practitioner, while also aiding the beginner in fully understanding this important branch of molecular biology.

Key Features

  • Presents the latest information covering all aspects of working with RNA, delivering a holistic understanding of this leading field in molecular biology
  • Builds from basic information on RNA techniques to in-depth protocols for specific applications
  • Features new chapters on RNA sequencing and RNA in situ hybridization
  • Includes new material on RNA clinical applications and innovations, including RNA therapeutics and RNA vaccines, with particular relevance to coronavirus
  • Comprises the latest developments in transcriptomes and bioinformatics, with new material on computational RNA biology, RNA CHiP analysis, aptamer biology and RNA epigenetics


Researchers and investigators in molecular biology and biochemistry, biomedicine, and related fields. Graduate and postdoctoral students in molecular biology, biomedicine, and related life science programs

Table of Contents

  • Preface
    Preface to Fourth Edition
    Preface to Third Edition
    Preface to Second Edition
    Preface to First Edition
    List of Tables
    List of Figures
    1. An introduction to Anaesthesia and Anaesthetic Equipment
    2. Anaesthetic agents
    3. Monitoring and Managing Anaesthesia
    4. Specialised techniques
    5. Analgesia and Post-operative care
    6.  Anaesthesia of Common Laboratory Species 
    Appendix 1. Physiological data
    Appendix 2. Estimation of use of inhalational agents and carrier gases
    Appendix 3. Calculations of dilutions of anaesthetic mixtures for small rodents
    Appendix 4. Manufacturers of equipment and other items illustrated or cited in the text

Product details

  • No. of pages: 960
  • Language: English
  • Copyright: © Academic Press 2022
  • Published: November 22, 2022
  • Imprint: Academic Press
  • Paperback ISBN: 9780323902212
  • eBook ISBN: 9780323916561

About the Author

Robert Farrell, Jr.

Dr. Robert Farrell is a bench-current scientist who has 35 years of experience working with RNA in the study of transcriptional and posttranscriptional regulation of gene expression in a variety of model systems. He is also experienced in animal cell culture methods. Prior to joining the faculty at Penn State University, he operated a biotech education and service firm, winning the 1998 Small Business Contractor of the Year award from the U.S. Department of Agriculture. He is the recipient of campus- and college-wide awards for excellence in teaching, and has extensive experience running RNA and specialized biotechnology hands-on laboratory training programs all over the world. He often serves as a consultant within the pharmaceutical and biotech industries. Dr. Farrell received his Ph.D. and M.S. degrees from The Catholic University of America and his B.S. in Biology from Providence College. Dr. Farrell currently serves as the campus academic officer at Penn State York.

Affiliations and Expertise

Bench-Current Scientist, Pennsylvania State University, York, PA, USA

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