
RNA Methodologies
A Laboratory Guide for Isolation and Characterization
Description
Key Features
- Presents the latest information covering all aspects of working with RNA, delivering a holistic understanding of this leading field in molecular biology
- Builds from basic information on RNA techniques to in-depth protocols for specific applications
- Features new chapters on RNA sequencing and RNA in situ hybridization
- Includes new material on RNA clinical applications and innovations, including RNA therapeutics and RNA vaccines, with particular relevance to coronavirus
- Comprises the latest developments in transcriptomes and bioinformatics, with new material on computational RNA biology, RNA CHiP analysis, aptamer biology and RNA epigenetics
Readership
Table of Contents
1. RNA and the Cellular Biochemistry Revisited
2. Creating a Ribonuclease-Free Environment
3. Stringency: Conditions That Influence Nucleic Acid Structure
4. RNA Isolation Strategies
5. Isolation of Polyadenylated RNA
6. The Truth About Tissues
7. Going Green: RNA and the Molecular Biology of Plants
8. Quality Control for RNA Preparations
9. cDNA: A Permanent Biochemical Record of the Cell
10. RT-PCR: A Science and an Art Form
11. Quantitative PCR Techniques
12. miRNA and Other Noncoding RNAs
13. RNA Interference and RNA Editing
14. Electrophoresis of RNA
15. Photodocumentation and Image Analysis
16. Northern Analysis
17. Nucleic Acid Probe Technology
18. Quantification of Specific mRNAs by Nuclease Protection
19. Analysis of Nuclear RNA
20. RNA In-situ Hybridization
21. Array Analysis of Gene Expression
22. Nonarray Methods for Global Analysis of Gene Expression
23. Transcriptomes and Bioinformatics
24. RNA-seq: The Premier Transcriptomics Tool
25. RNA Biomarker Discovery and)
26. Functional Genomics and Transcript Profiling
27. A Few RNA Success StoriesAppendix
A. Maintaining Complete and Accurate Records
B. Converting Mass to Moles
C. Removal of DNA from an RNA Sample
D. Removal of RNA from a DNA Sample
E. Trypsinization Protocol for Anchorage-Dependent Cells
F. Electrophoresis: Principles, Parameters, and Safety
G. Disposal of Ethidium Bromide and SYBR Green Solutions
H. Polyacrylamide Gel Electrophoresis
I. Centrifugation as a Mainstream Tool for the Molecular Biologist
J. Silanizing Centrifuge Tubes and Glassware
K. Dot Blot Analysis
L. Deionization of Formamide, Formaldehyde, and Glyoxal
M. Useful Stock Solutions for the Molecular Biologist
N. Genomes and Proteomes
O. Useful SI Units
P. Common Abbreviations
Q. Selected Suppliers of Equipment, Reagents, and Services
R. Trademark Citations
Product details
- No. of pages: 920
- Language: English
- Copyright: © Academic Press 2022
- Published: October 1, 2022
- Imprint: Academic Press
- Paperback ISBN: 9780323902212