RNA Methodologies

3rd Edition

A Laboratory Guide for Isolation and Characterization

Print ISBN: 9780122496967
eBook ISBN: 9780080454764
Imprint: Academic Press
Published Date: 1st April 2005
Page Count: 688
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This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project.

RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5’ and 3’ RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis.

Key Features

  • Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center
  • Includes classic and contemporary techniques
  • Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects


Researchers, graduate students and lab technicians in the fields of molecular biology, cell biology, and biochemical genetics.

Table of Contents

RNA and the Cellular Biochemistry Revisited Transcription and the Organization of Eukaryotic Genes Messenger RNA Resilient Ribonucleases RNA Isolation Strategies The Truth About Tissues Isolation of Polyadenylated RNA Quality Control for RNA Preparations Dot Blot Analysis Electrophoresis of RNA Photodocumentation and Image Analysis Northern Analysis Nucleic Acid Probe Technology Practical Nucleic Acid Hybridization Principles of Detection Quantification of Specific mRNAs by Nuclease Protection Analysis of Nuclear RNA cDNA Synthesis RT-PCR Quantitative PCR Techniques Transcript Subtraction Methods mRNA Differential Display High Throughput Analysis of Gene Expression RNA Interference Genomes, Transcriptomes, Proteomes, and Bioinformatics An RNA Paradigm


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© Academic Press 2005
Academic Press
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Praise for the third edition @qu:"This third edition of RNA Methodologies is an updated version of a laboratory guide edited for the first time in 1993; it represents an exhaustive collection of tried, tested, and optimized protocols for isolation and chracterization of RNA. The third edition of RNA Methodologies is a well-documented guide for any researcher who has to manage RNA studies. Each chapter of this book provides a rationale to help in the decision-making process...The inclusion of troubleshooting hints and annotations based on personal experience are provided to improve the method(s). This laboratory guide offers protocols on traditional and new technologies, with a focus on how to use the expedient technique for achieving an experimental goal in the study of gene expression." @source:--Dr. Patrick Pagesy at INSERM for HEMOGLOBIN