Reprogramming the Genome: Applications of CRISPR-Cas in non-mammalian systems part B

Reprogramming the Genome: Applications of CRISPR-Cas in non-mammalian systems part B

1st Edition - April 29, 2021
  • Editor: Vijai Singh
  • eBook ISBN: 9780323852982
  • Hardcover ISBN: 9780323852975

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Reprogramming the Genome: Applications of CRISPR-Cas in Non-mammalian Systems Part B, represents the collation of chapters written by eminent scientists worldwide. CRISPR-Cas9 system is an RNA-mediated immune system of bacteria and archaea that protects from bacteriophage infections. It is one of the revolutionized technologies to uplift biology to the next stages. It is a simple, rapid, precise, and cost-effective tool for genome editing and regulation of a wide range of organisms. It has gained scientific and public attention worldwide. This volume mainly covers insect cell line, protozoans, zebrafish, drosophila, CRISPRi, patents as well as technology transfer, and many more. This book is a key source of information available in a single volume. This book will be useful for not only beginners in genome engineering, but also students, researchers, scientists, policymakers, and stakeholders interested in harnessing the potential of reprogramming of the genomes in several areas.

Key Features

  • Offers basic understanding and a clear picture of genome editing CRISPR-Cas systems in different organisms
  • Explains how to create an animal model for disease diagnosis/research and reprogram CRISPR for insect cell line, protozoans, zebrafish, drosophila, and many more
  • Discusses the advances, patents, applications, challenges and opportunities in CRISPR-Cas9 systems in basic sciences, biomedicine, molecular biology and many more


Beginners in area of genome engineering area, but also researchers, students, scientists, clinicians, stakeholders, policy makers and practitioners and many more

Table of Contents

  • 1. Development of insect cell line using CRISPR technology
    Ekene Emmanuel Nweke and Deepak B. Thimiri Govinda Raj

    2. CRISPRing protozoan parasites to better understand the biology of diseases
    Apurva Kirti, Manish Sharma, Komal Rani and Abhisheka Bansal

    3. CRISPR based genome editing of zebrafish
    Preeti Sharma, B. Sharan Sharma and Ramtej J. Verma

    4. Using CRISPR-Cas9 based genome engineering tools in Drosophila melanogaster
    Deepti Trivedi

    5. CRISPR interference and its applications
    Seyedkhashayar Ghavami and Amir Pandi

    6. CRISPR-Cas systems: Challenges and future prospects
    Nisarg Gohil, Gargi Bhattacharjee, Navya Lavina Lam, Samuel D. Perli and Vijai Singh

    7. Patents and technology transfer in CRISPR technology
    Lichelle Grobler, Essa Suleman and Deepak B. Thimiri Govinda Raj

Product details

  • No. of pages: 198
  • Language: English
  • Copyright: © Academic Press 2021
  • Published: April 29, 2021
  • Imprint: Academic Press
  • eBook ISBN: 9780323852982
  • Hardcover ISBN: 9780323852975

About the Serial Volume Editor

Vijai Singh

Dr. Vijai Singh is an Associate Professor and Dean (Research & Innovation) at School of Sciences, Indrashil University, Rajpur, Mehsana, Gujarat, India. He was an Assistant Professor in the Department of Biological Sciences and Biotechnology at the Institute of Advanced Research, Gandhinagar, India and also an Assistant Professor in the Department of Biotechnology at the Invertis University, Bareilly, India. Prior to that, he was a Postdoctoral Fellow in the Synthetic Biology Group at the Institute of Systems and Synthetic Biology, Paris, France and School of Energy & Chemical Engineering at the Ulsan National Institute of Science and Technology, Ulsan, South Korea. He received his Ph.D. in Biotechnology (2009) from the National Bureau of Fish Genetic Resources, Uttar Pradesh Technical University, Lucknow, India with a research focus on the development of molecular and immunoassays for diagnosis of Aeromonas hydrophila. His research interests are focused on building novel biosynthetic pathways for production of medically and industrially important biomolecules. Additionally, his laboratory is working on CRISPR-Cas9 tools for genome editing. He has more than 10 years of research and teaching experience in synthetic biology, metabolic engineering, bioinformatics, microbiology, and industrial microbiology. He has published 79 articles, 49 chapters, 12 books and 4 patents. He serves as an associate editor, editorial board member, and reviewer of several peer-reviewed journals. He is also a member of the Board of Study and Academic Council of Indrashil University and is the Member Secretary of the Institutional Biosafety Committee (IBSC) at the same University.

Affiliations and Expertise

Associate Professor and Dean, Department of Biosciences, School of Science, Indrashil University, Rajpur, Mehsana, Gujarat, India