Regulatory Mechanisms in Lymphocyte Activation

List of Contributors

Preface

Acknowledgments

Symposium I. Parameters of Activation and Regulation

An Integrative Approach to Activation and Sequential Metabolic Regulation of the Immune Response

Catecholamine Hormone Receptors and Cell Division

The Role of Cation Flux in Triggering and Maintaining the Stimulated State in Lymphocytes

Parameters of Lymphocyte Activation

Ribosome Utilization and Regulation of Protein Synthesis during Lymphocyte Activation

Symposium II. Positive Regulation and Facilitation of Lymphocyte Response

Role of Adherent Cell Products in the Immune Response

Genetic Regulation of Macrophage-T Lymphocyte Interaction

Regulation of B Lymphocytes by T Cell Membrane Gangliosides

Specific and Non-Specific Activities in Dialyzable Transfer Factor

Symposium III. Suppression of Lymphocyte Responses

Maintenance of the Resting State and Potential Regulators of the Proliferative Phase

Serum Factors Which Suppress the Immune Response

Suppression of Immune Responses by Products of Activated T Cells

Dysfunction of Suppressor Mechanisms Associated with Immunodeficiency and Autoimmunity

Immunosubversive Activity of Tumor Cells as an Escape Mechanism

Short Papers. Membrane Determinants and Receptors

T Cell Activation Induced by Cross-Linking of Anti-T Cell Directed Antibodies with Anti-Immunoglobulin

Concanavalin A Induced Triggering of Allosensitized Memory Lymphocytes into Secondary Cytotoxic Lymphocytes

Identification and Characterization of the Chemical Moiety of LSP Responsible for C3H/HeJ Spleen Cell Mitogenicity

Isolation of a Mitogenetic Protein from Wheat Germ with the Same Specificity for the Lymphocyte Membrane as Wheat Germ Agglutinin

A Receptor for Normal Human Serum Low Density Lipoproteins on Peripheral Blood Lymphocytes

Characterization of the Mouse Lymphocyte Receptor for Human ß2-Microglobulin

Mechanism of Anti-µ Induced Suppression of LPS Induced Immunoglobulin Synthesis

The Mechanism of FC Receptor-Mediated Inhibition of B Lymphocyte Activation by Antigen

Decreased Responsiveness of Lymphocytes Tagged with Dinitrofluorobenzene (DNFB) to Phytomitogens

Inhibition of Macrophage-Induced Thymic Lymphocyte Maturation by Cytochalasin B

The Insulin Receptor on the T Cell: A Marker of Activation after Stimulation

A Rosette Marker for Mitogen Activated Human Lymphocytes

Labeling of Human Leukocyte Surface Receptors with Microspheres, in Giemsa Stained Preparations

Studies on the Human T Lymphocyte—Sheep Erythrocyte Receptor

Re-Evaluation of Receptors for IgG on Human Lymphocytes

Rabbit T and B Lymphocytes: Surface Markers and Functional Characteristics

Reactions of Human Peripheral Blood Lymphocytes with C5b-9 Complement Complex-Specific Antibodies

Identification of Human Lymphocyte Subpopulations by Rapid Microrosette Technique

Separation Methods, Surface Markers, and Cytotoxic Capabilities of Human Eosinophils

Effect of Ficoll-Hypaque Separation on Activation and DNA Synthesis of Human Blood Lymphocytes

Augmentation of in Vitro Generated Cell Mediated Cytotoxicity by Neuraminidase

Isolation of Tumor Associated Antigen(s) from a Moloney Virus Induced Leukemia (MBL-2)

Short Papers. Major Histocompatibility Complex

Expression of H-2 Specificities and of MuLV Envelope Antigens on Murine Tumors

Antigen Mapping for T Cell Recogniton: Immune Response Gene Control of Determinant Selection

Specificity of Anamnestic Responsiveness in Vitro to Antigens under Histocompatibility-linked IR Gene Control in Rats

The TYN-PLOP Phenomenon in HLA-D Typing

MLR Inhibition, HLA-D Typing Using Homozygous Typing Cells and in Vitro Primed Lymphocytes versus a B Cell Specific Antigenic System

Primed LD Typing: Analysis of the HLA-D Complex

In Vitro Stimulation of Ur primed Lymphocytes by Allogeneic

Semi-Soluble Lymphocyte Membrane Fragments

The Primed Canine MLC: Allograft Immunity in Vitro

Primary in Vitro Sensitization of Isogeneic and Allogeneic

Murine Lymphocytes to Normal and SV40-Transformed BALB/c 3T3 Cells

Extraction of la-Like Antigen from Cultured Human B

Lymphoblasts and Its Expression on Leukemic Cells

Workshop Report. H-2 Restriction of T Cell Function

Short Papers. Lymphocyte Response

Processing of LPS by Cells Competent to Respond: Conversion to a Low Molecular Weight Form Strongly Mitogenic for C3H/HeJ Low Responders

Mitogenicity of Derivatives of the Iipoprotein from the Outer Membrane of Escherichia Coli

Lymphocyte Surface Glycosyltransferases

Functional Mosaicism of the Lymphocyte Plasma Membrane

The Specificity of Con A-Induced Decrease in Lymphocyte Potassium

Transmembrane K+ Turnover by Phytohemagluttinin (PHA)-Stimulated Human Lymphocytes

Differential Alteration of Membrane Transport in Non-Adherent Spleen Cells from Con-A Stimulated Mice

Effect of the Ionophore A23187 upon Lymphocyte Calcium Metabolism

Changes in the Iipid Components of Stimulated Human Lymphocytes

Fc Receptor Mediated Increases in Intracellular cAMP

A Single Second Signal for Mitogenesis?

Synthesis of Albumin by Rabbit Lymphoid Cells

In Vitro Modulation of Phytohemagglutinin-P Induced Blastogenesis by Parathyroid Hormone

Evidence for an Early Sulfhydryl Reagent Sensitive Step during Lymphocyte Activation

Factors Influencing Stimulation and Proliferation in Mitogen Stimulated Thymocytes

Factors Affecting the Measurement and Interpretation of in Vitro Lymphocyte Reactivity

Pitfalls in the Lymphocyte Proliferation Assay: Variations in Proliferation Kinetics and Cold Thymidine Pools

Is There Gene Amplification or Other Nonduplicative DNA Synthesis in Human Lymphocyte Activation?

Cellular Cooperation in the Release of DNA by Human Lymphocytes

Intermediates of DNA Synthesis of Mouse Thymocytes

Workshop Report. Kinetics of Dna Synthesis and Cell Proliferation in Lymphocyte Activation

Short Papers. Subpopulations of Immune Reactive Cells

Analysis of Surface Properties, Fine Structure and Organ

Distribution of Two Distinct T-Cell Subpopulations: Tµ and Ty

Proliferative Responsiveness of Two Distinct Human T-Cell Subpopulations to Con A, PHA and Alloantigens

Separation of Tumor Enhancing Murine Thymocytes by Agglutination with a Peanut Lectin (PNA)

Spontaneous Cytotoxicity by Human Peripheral Blood Lymphocytes

Studies of Monocyte Fc Receptor Avidity and Monocyte Mediated Antibody Dependent Cellular Cytotoxicity (MMADCC) in Man

Binding of Immunoglobulin-Bearing Lymphoid Cells to Allogeneic and Synegeneic Target Cells

Antibody Dependent Cell Mediated Cytotoxicity (ADCC) in Severe Combined Immunodeficiency (SCID)

Characterization of the Effector Cell in an Antibody Dependent Cell Mediated Cytotoxicity System

Regeneration and Proliferation in Vitro of Cytolytic T Lymphocytes Specific for MSV-Associated Tumor Antigens

Cytotoxicity of Human Lymphocytes in Cell-Mediated Lympholysis (CML) and Antibody-Dependent Lymphocytotoxicity (ADL)

Different Mechanisms for Natural Killer (NK) and Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC) by Fc-Receptor Bearing Lymphocytes

In Vitro Induction of Natural Killer (NK) and Killer (K) Cells

Is There a Relationship between Effector-Target Cell Binding and Cell-Mediated Cytotoxicity?

Mechanisms of Target Cell Killing by Activated Lymphocytes in Vitro

Identification of Lymphotoxin (LT) As the Mediator of Direct Cytolysis of L-929 Cells by Mitogen Activated Human Lymphocytes in Vitro

Fractionation of Human Lymphocytes on Linear Density Gradients

Primary in vitro Antibody Response from Human Peripheral Blood Lymphocytes

Plaque Forming Cell Assay for the Measurement of Polyclonal Activation of Human Peripheral Blood Lymphocytes. Cellular Requirements and Interactions

Effect of Antigen on Localization of Immunologically Specific B Cells

Identification of Five Human Lymphocyte Subpopulations by Differential Labeling with Bacteria

The Toxicity of Cyclophosphamide for T and B Cells

In Vitro Growth of Canine and Murine Granulocyte-Monocyte, Fibroblast and Lymphocyte Colonies: Preliminary Results

Workshop Report. Leukocyte Separation Techniques

A Critique of Techniques of Macrophage-Monocyte Depletion in Studies of Human Peripheral Blood Mononuclear Leukocyte (MNL) Function

Technical Considerations for the Separation of Human Peripheral Blood Lymphocyte Subpoputations by Rosetting with Sheep Erythrocyte

Modifications of Anti-Fab Immunoabsorbent Separation of Human Peripheral Blood Mononuclear Cells

Separation of Human Lymphocytes by Their FC Receptor

Workshop Conclusions

Short Papers. Cell Interactions

Generation of Cytotoxic T Cells Requires Antigen-Specific Helper T Cells

In Vitro Induction and Assay of Helper T-Cells Using Unprimed Purified Mouse Cell Populations

Periodate-Induced Blast Transformation in Mixtures of Rat Lymphoid Cells

Enhancement of Weak MLC Type Reactions by Hydiophilic Polymers

Role of Rat Lymphocyte Subpopulations in the Mixed Lymphocyte Culture (MLC)

Inhibition of the Human Mixed Lymphocyte Culture (MLC) Interaction by Cytotoxic Agents Conjugated to Anti-Lymphocyte Globulin

Virus-Macrophage-Lymphocyte Interactions

Workshop Report. Macrophage-Lymphocyte Interaction

Short Papers. Ontogeny of Lymphocytes

Thymosin-Induced Enhancement of MIF Production by Peripheral Blood Lymphocytes of Thymectomized Guinea Pigs

T Cell Function in Congenitally Asplenic (Dh/+) Mice

Interactions of Human Neonatal T and B Lymphocytes and Evidence for Functional Immaturity

Cell-Mediated Lympholysis by Human Neonatal Lymphocytes

To the Question of Null Cells in the Avian Bursa of Fabricius

Short Papers. Regulatory Mechanisms

Spontaneous Cytotoxicity by Human Peripheral Blood Lymphocytes Due to a Time Dependent Loss of Suppressor Cells

Regeneration of Effector Function in Vitro

Role of H-2 CD Antigens in Secondary Responses

Analysis of Secondary MLR by Titration of in Vitro Primed Human Lymphocytes

Enhancement of Pinocytosis in the Macrophage by Macrophage Activating Factor

Migration of Normal Human Lymphocytes Cultured in the Presence of Candida Albicans Extract

Mechanism of Leukocyte Adherence Inhibition

Modulation of Human EA Rosette Formation and Antibody-Dependent Cell-Mediated Cytotoxicity by Promethazine and Agents Which Alter Cyclic Nucleotide Levels

Cellular Cytotoxicity Induced by Calcium Ionophore A23187

The Role of Opsonins in Phagocytosis and Intracellular Killing of Gram-Negative Bacteria by Human Polymorphonuclear Leukocytes (PMN)

Spontaneous Lymphocyte Proliferation in Hodgkin's Disease

Activation of Isolated T Cells in Chronic Lymphocytic Leukemia (CLL) by Mitogens

Purine Metabolism in Severe Combined Immunodeficiency

Production in MLC Reaction as a Measure of Immunocompetence in Immunodeficiency Diseases

Further Characterization of Neoplastic Helper T Cells from

Patients with the Sdzary Syndrome

Adjuvant Properties in Human Dialysable Leukocyte Extracts (DLE) Containing Transfer Factor

Interaction of Dextran-Triggered Murine Splenic Cell Supernatants and Allogeneic Effect Factor

T-Cell Mediator Induction of Non-T Cell Cytotoxicity

Anti-IgD (ä) and F(ab')2 Anti-IgD Activated B Cells Enhance PHA Responsiveness

Kinetics of the Potentiation of DNA Synthesis by "Lymphocyte Activating Factor"

Stimulation of Human Lymphocytes: Activation by a Sodium Periodate Induced Growth Factor

The Effect of Various Thymic Extracts on the in Vitro Immune Response against SRBC and on Mitogen Responses and IntracellularcAMP Levels of T Cells

Suppression of the Immune Response to UV Induced Tumors by UV Light

Effect of Cyclosporin A on Murine Lymphoid Cells

Failure of Levamisole to Stimulate Humoral or Cellular Immunity to Sheep Erythrocytes in Normal or T Cell Deprived Mice

Human Monocyte-Lymphocyte Interaction Mediated by Concanavalin A: The Effect of Levamisole

Nonspecific Suppression of the Initiation of the Primary Immune Response in Vitro

Regulation of Autoimmunity by the Mode of Presentation of Autoantigen to Lymphocytes

Serum Factors Affecting the Incorporation of [3H] Thymidine by Lymphocytes Stimulated by Aptigen: Increased Divergence between Antigen Dose-Response Curves in Heated and Control Serum with Cells from Rabbits Immunized to Increase the Proportion of High Specificity Cells

Suppression of the in Vivo Humoral Immune Response by a Species of Normal Human Serum Low Density lipoprotein

The Bioregulatory Properties of Serum Low Density lipoprotein Inhibitor (LDL-IN) on the Generation of Killer T Cells

Inhibition of Antigen-Induced Human Lymphocyte Proliferation by Daudi Cell Line Supernatant

Naturally Tolerant Pigs—Reactivity of Peripheral Blood Lymphocytes (PBL) in Mixed Lymphocyte Culture (MLC) and Cell Mediated Cytolysis (CMC)

Two Distinct Human T-Cell Subpopulations: Suppressor Activity on PWM-Induced B-Cell Differentiation Is Restricted to T Cells Bearing Fc-IgG Receptors

Heterogeneity among Suppressor T Cells

The Persistence of in Vivo and in Vitro Immune Responses in Thymectomized Rabbits

Evidence for T Suppressor Cell Function in a Thymus Independent Mitogenic Response to Lipopolysaccharide

Regulation of the Immune Response of Rabbit Lymphocytes by Antigen Activated Suppressor Cells

In Vitro Generation of Specific and Non-Specific Suppression of Cell Mediated Cytotoxicity (CMC)

Radiation Sensitivity of the Mechanism Inducing Non-Specific Desensitization (Anergy) in Guinea Pigs

Inhibition by Measles Virus of Lymphocyte Stimulation in Vitro

Suppression of the HGG Response in Normal Mice with a Spleen Cell Lysate from Tolerant Mice

Evidence for Aberrant Immunoregulation of NZB Autoantibody-Secreting Precursor B Lymphocytes

Failure of Concanavalin A-Stimulated Lymphocytes to Suppress Responsiveness to PHA and Allogeneic Cells as a Marker of Disordered Immunoregulation

Immunoglobulin Class Specific Suppressor T Cells

Enhancement of PGEi Sensitivity in Macrophages

A Comparison of Alveolar and Peritoneal Macrophages as Accessory Cells in PHA Induced Proliferation of Guinea Pig Lymphocytes

Adherent Cell Populations and the Responses to PHA, Con A, and PPD

The Effect of Macrophages on TdR Incorporation into Mitogen Stimulated Rat Lymphocytes

Macrophage-Monocyte Regulation of the Mitogenic Activation of X-Rayed, Purified Human T Lymphocytes

Macrophage Mediated Inhibition of Mitogen-Induced Lymphocyte Blastogenesis in Mice

Adherent Suppressor Cells in Normal Rat Spleens

Characterization of the Adherent Splenic Suppressor Cells

Presence of Splenic Suppressor Cells in Allograft Enhanced Rats

Workshop Report. Recent Advances and Questions in Lymphocytotoxicity

Workshop Report. Suppression

Index