Recombinant DNA Laboratory Manual, Revised Edition

Recombinant DNA Laboratory Manual, Revised Edition

1st Edition - January 22, 1992

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  • Authors: Judith Zyskind, Sanford Bernstein
  • Paperback ISBN: 9780127844015
  • eBook ISBN: 9780323137874

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Description

The latest edition of this introductory benchtop manual is up-to-date, affordable, and easy-to-follow. This text is perfect for your two-quarter or one semester course in Recombinant DNA Techniques and is specifically designed to lead your student or technician, who is a newcomer to molecular biology, from the basic skills of growing and maintaining bacterial colonies through plasmid DNA isolation, cloning, DNA sequencing, and hybrid detection.

Key Features

  • Comb-bound, three-column, large 9-1/4" x 7-1/2" format
  • Exercises contain explanatory material and margin notes that pinpoint critical steps and important concepts
  • Necessary reagents and equipment are presented in a checklist at the beginning of each protocol
  • Techniques for bacteria are complemented with those for Drosophila
  • Each experiment has been tested in the laboratory by students for five years
  • Features a complete chapter on computers in the molecular biology laboratory
  • Presents helpful appendixes on safety in the laboratory, frequently used ancillary techniques, and recipes for buffers, media, and strains

Readership

Students in introductory courses in recombinant DNA and laboratory technicians in academia, government, and industry

Table of Contents

  • Lab I: Bacterial Growth Parameters.
    Lab II: Isolation and Analysis of Bacterial and Drosophila Chromosomal DNA.
    Lab III: Plasmid DNA Isolation and Agarose Gel Analysis.
    Lab IV: Introduction of DNA into Cells.
    Lab V: Tn5 Mutagenesis of pBR329.
    Lab VI: DNA Cloning in M13.
    Lab VII: DNA Sequencing.
    Lab VIII: DNA Gel Blotting, Probe Preparation, Hybridization, and Hybrid Detection.
    Lab IX: Lambda Phage Manipulations.
    Lab X: The Polymerase Chain Reaction Technique. Appendixes:
    D.W. Smith, Use of Computers in a Molecular Biology Laboratory.
    Safety in the Recombinant DNA Laboratory.
    Solutions.
    Strains and DNA.
    Molecular Biology Reagent Suppliers.
    Frequently Used Enzymes.
    Frequently Used Techniques Not Described in Labs.
    Chapter References.
    Index.

Product details

  • No. of pages: 224
  • Language: English
  • Copyright: © Academic Press 1992
  • Published: January 22, 1992
  • Imprint: Academic Press
  • Paperback ISBN: 9780127844015
  • eBook ISBN: 9780323137874

About the Authors

Judith Zyskind

Affiliations and Expertise

San Diego State University, California, U.S.A.

Sanford Bernstein

Affiliations and Expertise

San Diego State University, California, U.S.A.

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