Recombinant DNA Laboratory Manual, Revised Edition

1st Edition

Print ISBN: 9780127844015
eBook ISBN: 9780323137874
Imprint: Academic Press
Published Date: 22nd January 1992
Page Count: 224
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The latest edition of this introductory benchtop manual is up-to-date, affordable, and easy-to-follow. This text is perfect for your two-quarter or one semester course in Recombinant DNA Techniques and is specifically designed to lead your student or technician, who is a newcomer to molecular biology, from the basic skills of growing and maintaining bacterial colonies through plasmid DNA isolation, cloning, DNA sequencing, and hybrid detection.

Key Features

@introbul:The most useful features of this manual for your classroom and laboratory are: @bul:* Comb-bound, three-column, large 9-1/4" x 7-1/2" format

  • Exercises contain explanatory material and margin notes that pinpoint critical steps and important concepts
  • Necessary reagents and equipment are presented in a checklist at the beginning of each protocol
  • Techniques for bacteria are complemented with those for Drosophila
  • Each experiment has been tested in the laboratory by students for five years
  • Features a complete chapter on computers in the molecular biology laboratory
  • Presents helpful appendixes on safety in the laboratory, frequently used ancillary techniques, and recipes for buffers, media, and strains


Students in introductory courses in recombinant DNA and laboratory technicians in academia, government, and industry.

Table of Contents

Lab I: Bacterial Growth Parameters. Lab II: Isolation and Analysis of Bacterial and Drosophila Chromosomal DNA. Lab III: Plasmid DNA Isolation and Agarose Gel Analysis. Lab IV: Introduction of DNA into Cells. Lab V: Tn5 Mutagenesis of pBR329. Lab VI: DNA Cloning in M13. Lab VII: DNA Sequencing. Lab VIII: DNA Gel Blotting, Probe Preparation, Hybridization, and Hybrid Detection. Lab IX: Lambda Phage Manipulations. Lab X: The Polymerase Chain Reaction Technique. Appendixes: D.W. Smith, Use of Computers in a Molecular Biology Laboratory. Safety in the Recombinant DNA Laboratory. Solutions. Strains and DNA. Molecular Biology Reagent Suppliers. Frequently Used Enzymes. Frequently Used Techniques Not Described in Labs. Chapter References. Index.


No. of pages:
© Academic Press 1992
Academic Press
eBook ISBN:
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@qu:"The manual is bound with a comb and is sufficiently small that it can be conveniently used at the work bench. The protocols are clearly written, with separate layout of materials and instructions, much like a cookbook recipe. Some of the protocols are written so that students can fill in appropriate information. Wide margins provide plenty of space for notes, and some of the margins already contain helpful information about calculations, special handling, and precautions." @source:--ANALYTICAL BIOCHEMISTRY @qu:"This book contains very interesting and practical information about the methodology of recombinant DNA which is one of the most useful techniques at present in molecular biology... This manual would be very useful for molecular biologists, physiologists, biochemists, and advanced students." @source:--JOURNAL OF STEROID BIOCHEMISTRY @qu:"This is the most appropriate text available for the two week intensive course for professionals that we offer. The protocols are excellent with the perfect amount of background information and experimental detail." @source:--LINDA STRAUSBAUGH, The University of Connecticut @qu:"It covers a wide range of topics, covers them thoroughly, and eliminates the need for lab handouts." @source:--S. ACKERMAN, University of Massachusetts