1st Edition - May 15, 2022
This is the Latest Edition
  • Editors: Natalia Jura, James Murphy
  • Hardcover ISBN: 9780323915410

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Pseudokinases, Volume 666, the latest release in the Methods in Enzymology serial, highlights new advances in the field with this new volume presenting interesting chapters, including the Production and Purification of the PEAK pseudokinases for structural and functional studies, Structural biology and biophysical characterization of Tribbles pseudokinases, Detecting endogenous TRIB protein expression and its downstream signaling, Analysis of human Tribbles 2 pseudokinase, Expression, purification and examination of ligand-binding to IRAK pseudokinases, Characterization of pseudokinase ILK-mediated actin assembly, Biochemical examination of Titin pseudokinase, Approaches to study pseudokinase conformations, CRISPR editing cell lines for reconstitution studies of pseudokinase function, and much more.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in Methods in Enzymology serials
  • Includes the latest information on Pseudokinases


Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

  • 1. Production and Purification of the PEAK pseudokinases for structural and functional studies
    Isabelle S. Lucet
    2. Structural biology and biophysical characterisation of Tribbles pseudokinases
    Peter Mace
    3. Detecting endogenous TRIB protein expression and its downstream signalling
    Karen Keeshan
    4. Analysis of human Tribbles 2 pseudokinase
    Patrick Eyers and John Harris
    5. Expression, purification and examination of ligand-binding to IRAK pseudokinases
    Yogesh Kulathu and Sven Lange
    6. Characterization of pseudokinase ILK-mediated actin assembly
    Jun Qin
    7. Biochemical examination of Titin pseudokinase
    Olga Mayans
    8. Approaches to study pseudokinase conformations
    James Murphy, Yanxiang Meng and Cheree Fitzgibbon
    9. CRISPR editing cell lines for reconstitution studies of pseudokinase function
    James Murphy and Annette Jacobsen
    10. Proximity ligation approaches to define the interactomes of pseudokinases
    Dario R. Alessi and Toby Dite
    11. Determining the role of the pseudokinase domain in guanylyl cyclase A and B signaling.
    Lincoln Potter and Aaron Edmund
    12. Strategies to study and stimulate bacterial pseudokinases
    Seth Childers
    13. Dynamics of Kinases and Pseudokinases by HDX-MS
    Mark Lemmon, Joshua Sheetz and Yuko Tsutsui
    14. In-cell thermal shift assay for drug binding analysis to receptor pseudokinases
    Daniela Ungureanu
    15. Pseudoenzymology of Kinase Suppressor of Ras
    Arvin Dar
    16. Computational and evolutionary insights into pseudokinase mechanisms
    Natarajan Kannan
    17. Methods to identify small molecule allosteric modulators of the STRAD pseudokinase
    John Gordan
    18. Pharmacological targeting of the pseudokinase HER3
    Angus Cameron
    19. Considerations and analysis of the unusual pseudokinase BUBR1
    Sabine Elowe and Luciano Gama Braga
    20. The pseudokinase domain of receptor guanylyl cyclases: binding of ATP and allosteric regulation of guanylyl cyclase activity
    Sandhya Visweswariah
    21. Methods for discovering catalytic activities for pseudokinases
    Vincent Tagliabracci and Krzysztof Pawlowski
    22. Efficient Expression, Purification, and Visualization by Cryo-EM of Fully-Glycosylated Unliganded HER3
    Natalia Jura and Kliment Verba
    23. An effective strategy for ligand-mediated pulldown of the HER2/HER3 receptor complex and cryo-EM structure determination at low sample concentrations
    Natalia Jura
    24. Identification of small molecule binders for pseudokinases
    Stefan Knapp and Sebastian Mathea
    25. Identification and characterization of Tyk2-JH2 pseudokinase domain inhibitors
    Gregory Locke and Christopher Barbieri
    26. Monitoring the ATPase activity of Bud32 and its role in tRNA binding
    Jonah Beenstock and Frank Sicheri

Product details

  • No. of pages: 412
  • Language: English
  • Copyright: © Academic Press 2022
  • Published: May 15, 2022
  • Imprint: Academic Press
  • Hardcover ISBN: 9780323915410
  • About the Serial Volume Editors

    Natalia Jura

    Dr. Natalia Jura is an Associate Professor and an Investigator in the Department of Cellular and Molecular Pharmacology at the Cardiovascular Research Institute within the School of Medicine at the University of California, San Francisco (UCSF). She is also an Associate Director of the Quantitative Biosciences Institute at UCSF. Dr. Jura's group at UCSF focuses on understanding how soluble protein kinases and membraneassociated receptor kinases assemble into functional complexes and regulate their signaling through molecular interactions with regulatory proteins. Her group also investigates alternative non-catalytic roles of protein kinases as scaffolds in cellular signaling pathways. They apply this knowledge for design of small molecule inhibitors that target these poorly understood kinase functions in human diseases. Dr. Jura received her M.S in biochemistry from Jagiellonian University in Krakow, Poland and her Ph.D. in molecular and cellular biology from Stony Brook University.

    Affiliations and Expertise

    Assistant Professor, Cardiovascular Research Institute, Department of Cellular and Molecular Pharmacology, University of California, San Francisco, USA

    James Murphy

    James Murphy is Associate Professor and the head of the Inflammation Division at the WEHI (formerly known as The Walter and Eliza Hall Institute of Medical Research) in Melbourne and is closely associated with The University of Melbourne and The Royal Melbourne Hospital. James’ lab studies the protein-protein interactions that underpin signal transduction. Much of his work is focused on understanding the molecular mechanisms by which protein kinases and their relatives, pseudokinases, regulate cell signaling. Their work on the pseudokinase, MLKL, provided a template for developing a detailed understanding of how the remaining ~50 uncharacterized pseudokinases modulate cell signaling.

    Affiliations and Expertise

    Associate Professor, Head, Inflammation Division, Walter and Eliza Hall Institute of Medical Research, Victoria, Australia