Protides of the Biological Fluids

Protides of the Biological Fluids

Proceedings of the Thirty-Second Colloquium, 1984

1st Edition - January 1, 1985

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  • Editor: H. Peeters
  • eBook ISBN: 9781483279664

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An International Review Series Devoted to Proteins and Related Studies, Volume 32: Protides of the Biological Fluids documents the proceedings of the 32nd Colloquium held in 1984. This compilation is organized into three parts. Part I begins with an introduction to the defense mechanism of immunoglobulins, followed by a description of enzymes and proteins, such as lactoperoxidase and lactoferrin. The secretions that include CSF, colostrum, milk, female and male genital secretions, saliva, gastrointestinal juice, nasal and bronchoalveolar secretions, tears and synovial fluid are also elaborated. The tumor markers, which include ferritin, CEA, and TPA are dealt with in Part II. The immunotoxins, tumor imaging, and lymphoid malignancies are also covered. Part III is devoted to the advances in the separation of protides, including the staining procedure for protein detection, two-dimensional electrophoresis, and HPLC and affinity chromatography. This book is valuable to biology students and clinicians of disciplines related to advances in the protein field.

Table of Contents

  • Preface


    The Thirteenth Arne Tiselius Memorial Lecture

    Low Molecular Weight (LMW) Urinary Proteins

    Section A. Proteins in Secretions

    A.1. Immunoglobulins

    A.1.1. The Defense Mechanism

    A Model for Analysis of Protein Defense Mechanisms in Secretions

    Local Protein Synthesis and Passive Transfer in Human Body Fluid Formation

    Evidence for a Common Mucosal Immune System in Humans

    Complement Regulation in Tears

    A.1.2. IgA

    Biosynthesis of the IgA Dimer Receptor

    Binding of α1-Microglobulin to IgA

    Evidence for Migration of IgA Bearing Lymphocytes Between Peripheral Muscosal Sites

    Oral Adjuvants Enhance Salivary IgA Responses to Purified Streptococcus mutans Antigens

    IgA Antibodies to Oral and Ocular Bacteria in Human External Secretions

    The IgA Type Parietal Cell Antibody in Gastric Juice

    Molecular Analysis of Human Bile IgA

    IgA Dimers (D), Trimers (T) and Tetramers (Q): Transfer into Rat Bile and Reaction with Secretory Component (SC)

    Pathways of Polymeric IgA, Secretory Component, and Galactose-Exposing Proteins During Receptor-Mediated Endocytosis in Rat Hepatocytes: Ultrastructural Demonstration and Requirements for Intracellular Ligand Sorting

    Hepatic Uptake of Human Secretory IgA in Mice

    Secretory IgA1 and IgA2 Responses to Environmental Antigens

    IgA in the Bile, Saliva and Urine or Rats

    Changes in Size, Subclass and SC Content of Serum Immunoglobulin A in Diseases with High Polyclonal IgA

    Origin of IgA in Tears of Rats

    Androgen Regulation of IgA Levels in Tears of Rats

    Secretory Component and IgA in the Uterus of the Pregnant Rat

    Homogeneous Ig Components in Serum and Secretions of Aging C57BL/KaLwRij Mice

    The Wasted Mutant Mouse: A Model for the Study of IgA Deficiency in Ataxia-Telangiectasia

    A.2. Enzymes

    A.2.1. Lactoperoxidase

    The Peroxidase System in Human Tears

    The Biological Significance of Lactoperoxidase in Milk

    Characterization of Lactotransferrin Complexes in Human Milk

    Biochemical and Immunological Properties of Peroxidase from Human Parotid Saliva

    Some Structural and Functional Properties of Lactoperoxidase

    The Thiocyanate Binding to Lactoperoxidase

    Interactions of Sulphur in Proteins with Lactoperoxidase in the Oral Cavity

    A.2.2. Others

    Isolation of a Sputum Cysteine Protease Inhibitor

    Proteinases and Proteinase Inhibitors in Human Endometrial Secretion

    A.3. Lactoferrin

    Effect of Proteolysis on the Antimicrobial and Iron-Binding Properties of Human and Bovine Lactoferrins

    Lactoferrin as Complement Inhibitor in Synovial Fluid

    Effect of Enterochelin and pH on Uptake of Iron from Lactoferrin by Escherichia coli

    Lactoferrin and Fibronectin in Tears of Patients Undergoing Cataract Surgery

    A.4. C.S.F.

    The Science of Intra-Blood-Brain-Barrier IgG Synthesis

    Cerebrospinal Fluid IgG and T Cell Subpopulations in Multiple Sclerosis

    Detection of Oligoclonal IgG in Unconcentrated Cerebrospinal Fluid by Agarose Isoelectric Focusing and Double Antibody Avidin-Biotin-Peroxidase

    Methionine (Met)-Enkephalin and Leucine (Leu)-Enkephalin in CSF of Chronic Schizophrencis: Correlation with Psychopathology

    Inflammatory and Immune Reactions in the CSF of Patients with Herpetic Encephalitis

    Varicella Zoster Virus IgG Antibodies Produced by CSF Lymphocytes in Herpes Zoster

    The Use of Intrathecal IgG Synthetic Rates in Diagnosis

    Intrathecal CSF Ribonuclease in Neurological Diseases

    Semeiology of the Gammaglobulinic Pattern of Cerebrospinal Fluid in Children

    IgG Production within the Central Nervous System — A Critical Review of Proposed Formulae

    IgG Subclasses in Serum and Spinal Fluid from Patients with Various Neurological Diseases

    A Paraprotein IgA1 Occurring in Serum, Urine and CSF: Immunochemical Idiotypic Evidence of Synthesis in situ of the CNS

    Chromatofocusing of Cerebrospinal Fluid and Serum Immunoglobulin G

    Free Light Chains in Multiple Sclerosis

    Heterogenous Oligoclonal Pattern of CSF Immunoglobulins Analysed by Isoelectrofocusing on Polyacrylamide Gels: Due to Different Oligosaccharide Content?

    Different Distribution Pattern of Retinol-Binding Protein (RBP) and Prealbumin in Cerebrospinal Fluid (CSF) and Blood Serum

    Determination of C-Reactive Protein (CRP) in CSF by Enzyme Multiplied Immunoassay Technique (EMIT) and Immunonephelometry: Correlation of Both Methods

    A.5. Colostrum & Milk

    Electron Microscopic Study of Human Colostral Cells

    Presence of Antibodies to Food Antigens in Human Colostral Cells

    The Identification of A Human Milk Ganglioside which Inhibits A. Coli and V. Cholerae Enterotoxins

    Does Human Milk Contain Bombesin-Like Material?

    Reactions of Monoclonal Antibodies Against Membranes of Human Milkfat Globules with Uterine and Cervical Epithelia During Proliferative and Secretory Stages

    A.6. Female Genital Secretions

    Antibodies, Immunoglobulins and Complement in Oviductal Fluid of Rhesus Monkeys

    Immunolocalization of Secretory Component in Normal and Pathological Uterine Mucosa

    The Protein Composition of the Peritoneal Fluid During the Menstrual Cycle

    Rapid Effect of Ovarian Hormones on Secretory Component (SC) in Uterine Secretions of the Rat: The Involvement of RNA Synthesis in SC Production

    Pregnancy Associated Plasma Protein-A in Human Ovarian Follicular Fluid

    Placental Protein 5 in Human Ovarian Follicular Fluid

    A.7. Male Sexual Secretions

    Assessment of Antisperm Antibodies in Normal and Immunized Mice

    Male Accessory Sexual Glands Secretions and their Antithetical Role in Immunosurveillance

    Role for Beta 2 Microglobulin in Speciation

    Immunological Identification of Seminalplasmin in Tissue Extracts of Sex Glands of Bull

    Characterization of Angiotensin Converting Enzyme in Human Seminal Plasma

    Pregnancy Associated Plasma Protein-A in Human Seminal Plasma

    Quantitative Determination of Different Proteins in Normal and Pathological Semen

    Tissue Kallikrein in Human Seminal Fluid

    A.8. Saliva

    Blockage of Salivary Mediated Agglutination by Non-Specific Aggregated Immoglobulins

    Anti-Candida Properties of Natural Salivary Histidine-Rich and Synthetic Histidine Peptides: Relevance to Denture Stomatitis

    A.9. Gastro-Intestinal

    Localization of Cells Producing Immunoglobulins and Other Immune Factors in Human Gastric Mucosa

    Purification and Characterization of Gastric Mucus Antigen (GMA) From Mucinous Ovarian Cyst Fluid

    Immunohistological Detection of Gastric Mucus Antigen (GMA) in Human Fetal and Adult Tissues

    A Rapid and Reliable Kinetic Immunonephelometric Method (I.N.) for Routine Assay of Total IgA in Human Bile

    IgA Anti-HAV Antibody in Serum from Patients with Hepatitis A

    Quantitation of a Novel Serine Proteinase, Ingobsin by Precipitation with an Antibody that Leaves Enzymatic Activity Intact

    Maturation Dependent Changes in Nucleoside Phosphorylase (NP), Adenosine Deaminase (ADA) and ADA Complexing Protein (ADCP) in Intestinal Epithelial Cells

    Pancreatic Proteins: Characterization of a Component Devoid of Enzyme Activity

    Effects of Dietary Legume Proteins on the Morphology and Secretory Responses of the Rat Small Intestine

    Isolation of a Non-Mucin Macroglycoprotein from Meconium

    Use of Fecal Enzyme Determination in the Differentiation of Maldigestion and Malabsorption in Man

    Fecal Enzyme Changes in Human Liver Diseases

    Peritoneal Permeability for Small Proteins and Immunoglobulins Under CAPD

    A.10. Nasal

    Cells Producing Immunoglobulins and Other Immune Factors in Human Nasal Mucosa

    Secretory IgE Levels in Recurrent Nasal Polyposis

    A.11. Broncho-Alveolar

    Study of Immunoglobulins in Bronchoalveolar Lavage (BAL)

    Serum Type III Procollagen Peptide Levels in Cryptogenic Fibrosing Alveolitis

    Are Changes in Chemotactic Activity Involved in Asthmatic Processes?

    Cigarette Smoke Induced Tracheal Hypersecretion

    A.12. Tears

    Cellular Origin of Rabbit and Human Tear Proteins

    Comparative Protein Patterns in Tears of Several Species

    Enzymology of Tear Fluid

    Estimation of Lysozyme by Enzyme Immunoassay

    A.13. Synovial

    Synovial Fluid Fibronectin

    Section B. Tumor Markers and Targetting (Including Monoclonals)

    B.1. Immunotoxins

    Drug Targetting for 7 Neuroblastoma Patients Using Human Polyclonal Antibodies

    A Model for Cell Killing Using Radiolabelled Antibodies

    Antitumor Effect of Immunotoxins in Mouse and Human Tumor Models

    Binding, Uptake and Subcellular Distribution of Two Monoclonal Antibodies to Human Milk Fat Globule Membrane Studied on Human MCF 7 Breast Carcinoma Cells

    Use of an Anti-Tumour Monoclonal Antibody for Targeting Methotrexate

    Daunorubicin Conjugated to Galactosylated Neoglycoprotein as Drug Carrier Conjugate for Human Hepatomas

    Covalent Linkage of Anthracyclines to Macromolecular Carriers

    Targeting of Cytotoxic Drugs by Transferrin Receptors; Selective Killing of Acute Myelogenous Leukemia Cells

    Drug Targetting for Neuroblastoma: A Case Report

    Enhancement of the Specific Cytotoxicity of Anti-Carcinoembryonic Antigen Immunotoxins by Adenovirus and Carboxylic Ionophores

    B.2. Tumor Imaging

    Characteristics of First and Second Antibodies for Tumour Imaging

    Objective Interpretation of Antibody Scans

    Immunoscintigraphy with 111-Indium Conjugated Monoclonal Antibodies

    111-Indium-Labelled Antibodies for Tumour Detection

    Preparation of 111-Indium Labelled Antibodies

    The Localisation of an Anti-Tumour Monoclonal Antibody (791T/36) in Gastro-Intestinal Tumours

    Radioimaging of Prostatic Carcinoma with Prostatic Acid Phosphatase-Specific Polyclonal Antibodies

    Tissue Distribution of Radiolabeled Anti-CEA Monoclonal Antibodies in Man

    Immunoscintigraphy Using F(Ab')2 Fragments

    B.3. Immuno-Histochemistry

    Steroid Sulphatase Immunologically Detected: A New Tool in the Histopathological Investigation of Steroid Related Human Tumours?

    Analysis of Ewing Tumour with Panels of Monoclonal Antibodies

    Immunohistocytochemical Analysis of Gastrointestinal Carcinomas Using Immunoperoxidase and Immunogold Staining with Monoclonal Antibodies 19.9 and Anti CEA

    Tissue Localisation of Four Monoclonal Anti-CEA Antibodies in a Colon Tumour Xenograft

    Immunohistological Characterization of Monoclonal Anti-CEA Antibodies for Radioimmunoscintigraphy and Immunotherapy

    Immunocytochemical Characterization of Gastrointestinal Neoplasia by Monoclonal Antibodies

    Ultrastructural Localisation of Carcinoembryonic Antigen (CEA) on Ultrathin Cryosections of Different Tumours

    Generation of Monoclonal Antibodies Reactive with Glycoprotein Marker of Apocrine Epithelium and Metastatic Breast Carcinoma

    Identification of Proliferatively Active Cells by Monoclonal Antibodies Against DNA-Cytosine-5-Methyltransferase

    Monoclonal Antibodies to Cytokeratin Filaments in the Study of Tumor Histogenesis

    Monoclonal Antibodies to Cytokeratins in the Study of Premalignant Lesions of the Uterine Cervix

    Immunohistochemical Characterization of a Biotin-Labelled Monoclonal Antibody to Cytokeratins

    Immunohistological Studies of Human Sarcomas with Monoclonal Antibodies

    Fluorescent Probes for Tumour Cells

    Specific Identification of Normal and Malignant Human Prostatic Tissues Using Monoclonal Antibodies

    Detection with a Monoclonal Antibody an Estrogen Receptor Associated Antigen in Mammary and Ovarial Carcinomas

    Immunohistochemical Reaction of Three Monoclonal Antibodies to Human Milk Fat Globule Membrane with Human Normal and Tumoral Tissues

    Specific Enzyme Treatment is Required for Individual Monoclonal Antibodies in Immunohistochemistry with Formalin Fixed Sections

    Immunohistochemical Localization of the Immunodominant Differentiation Antigen Lacto-N-Fucopentaose 111 in Normal Adult and Fetal Tissues Utilizing Monoclonal Antibodies

    Foetal Pancreas Antigens as Possible Markers of Precancerous Stage in BOP Induced Pancreatic Carcinoma

    B.4. Receptors

    Pregnancy Proteins in Breast Cancer: Studies Using Tumour Cytosols and the Oestrogen-Sensitive Cell Line MCF-7

    Evidence for Alpha-Fetoprotein Receptors in the MCF-7 Human Breast Cancer Cell Line

    Secretory Component in Breast Cancer: Correlations with Patient Age and Hormone Receptor Levels in Primary Breast Tumours

    Transferrin Receptors and Tumor Cell Growth

    Specific Interaction of Rat Hepatic Asialoglycoprotein Receptor with Cell Surface Markers of Rat and Mouse Tumor Cells

    Transferrin Receptors, HLA-A,B,C, and Amnion Antigens in Breast Adenocarcinoma

    Characterization and Purification of Cytosolic and Nuclear Estrogen Receptors

    B.5. Tumor Markers

    B.5.1. New Markers

    Monitoring of Ovarian Carcinomas with CA 12.5 Antigen Radio Immuno-assay

    Cancer Antigen CA 125 in Serum of Patients with Ovarian Cancer

    CA 125 Seric Levels in Non Ovarian Pathologies

    The New Monoclonal Ovarian Cancer Antibody CA 12-5

    Value of CA 12.5 Antigen as Tumour Marker: Preliminary Results

    Clinical Follow-Up of Patients with Gastrointestinal Cancers Using CA 19-9 and CEA-Tests with Monoclonal Antibodies

    The Value of Centocor CA 19-9TM or Gastrointestinal Cancer Associated Antigen (GICA) in the Diagnosis and Prognosis of Colorectal Cancer

    Value of CA 19.9 Antigen as Tumor Marker: Preliminary Results

    A Novel 19,000 Membrane Glycoprotein Purified from Human Red Blood Cells Using a Monoclonal Antibody

    B5, A Monoclonal Antibody Against a New Tumour Marker on Human Erythrocytes

    Macro-Creatine Kinase Isoenzyme (Macro-CK), A Macromolecular Form of a Tumor-Associated Marker (CK-BB), in Hypothyroidism

    Serum Neuron-Specific Enolase Levels in Lung Diseases: A Valuable Marker For Small-Cell Lung Cancer

    Complement Split Product C3d as a Tumor Marker?

    A Sandwich-Radioimmunoassay for a New Antigen (MAM-6) Present in the Sera of Patients with Metastasized Carcinomas

    B.5.2. Ferritin

    Ferritin: Its Status as a Tumour Marker

    The Molecular Characteristics and the Significance of Oncofetal Ferritin in the Diagnosis of Breast Cancer

    The Use of Monoclonal and Polyclonal Antibodies for the Assay of Ferritin in Malignant Disease

    The Source of Serum Ferritin in Patients with Malignant Tumours

    Clinically Significant Correlations Between Blood Transfusions, Ferritin and Prognosis in Neuroblastoma

    The Distribution of Ferritin and Ferric Iron in the Spleens of Lymphoma Patients and Controls

    Serum Ferritin in Patients with Gynaecological Cancer

    Serum Levels of Ferritin, Lactoferrin and Lysozyme in Patients with Disseminated Non-Seminomatous Germ Cell Tumors are Influenced by Chemotherapy

    B.5.3. C.E.A.

    CEA: A Useful Prognostic Marker in Small Cell Lung Cancer

    Comparison of Poly- and Monoclonal Antibodies for Plasma Determination in Normal Individuals, Patients with Benign Lung Diseases and Patients with Lung Cancer

    Adenocarcinoma Polyclonal Antibody as a Diagnostic Tool of High Specificity

    Alteration of Circadian Variation in Serum Concentrations of Carcinoembryonic Antigen (CEA) in Cancer

    Serum CEA and PHI as Tumor Markers in the Follow Up of Breast Cancer Patients

    Significance of CEA Antibody Affinity for Recognition of Cancer by Plasma Determinations

    B.5.4. T.P.A.

    What is TPA? (Tissue Polypeptide Antigen): On the Relation Between TPA and Cytokeratins

    Monoclonal Anti-TPA Antibodies and Their Use for Immunohistochemical Demonstration of TPA

    Display of TPA in Interphase and Mitosis

    Staining for TPA and CEA as an Aid in Search for Primary Tumors and Micrometastases

    Tissue Polypeptide Antigen (TPA) in Patients with Breast Cancer — Longitudinal Study

    Tissue Polypeptide Antigen (TPA) as a Tumour Marker in Small Cell (SCLC) and Non-Small Cell (NSCLC) Lung Carcinomas

    TPA, CEA and CA-19-9 Tests in Surveilling Patients with Breast and Gastrointestinal Cancer

    Carcinoembryonic Antigen (CEA) and Tissue Polypeptide Antigen (TPA) in Normal and Neoplastic Human Tissues

    Preliminary Studies to Compare Sensitivity of Tissue Polypeptide Antigen (TPA) with Carcinoembryonic Antigen (CEA) as Universal Tumor Markers

    Serum Levels of TPA and CA 12-5 in Patients with Ovarian Carcinoma

    Prognostic Values of TPA, CEA and Their Combination by Product Formation in Different Cancer Locations

    B.5.5. Various Markers

    TN, A General Carcinoma-Associated Antigen

    Monoclonal Antibodies Specific for Human Thomsen-Friedenreich (T) and Tn Blood Group Precursor Antigens

    T-Antigens: Markers of Malignant Transformation of the Colon

    Preclinical and Clinical Evaluation of a Monoclonal Antibody to a Human Melanoma-Associated Antigen

    Clinical Relevance of Human Oncofetal Antigen (HOFA) as a Marker of Tumor Burden

    The Practical Usefulness of Tumor Markers in Oncology

    Fibronectin in Malignancies

    Tumor Specific Characteristics of a Pteridine — Binding Alpha1-Acid Glycoprotein (AGPM) and its Mitogenic Activity

    A Fresh Look at C Reactive Protein Measurements

    Demonstration of Immune Complexes and their Possible Role in Cancer

    Role of Immune Complexes in Tumor Associated Immunity of Patients with Prostatic Cancer

    Further Study of the S-100 Antigen in Neoplastic Tissues

    Detection of the Determinants on Blood Group H Antigen in Sera of Cancer Patients with Monoclonal Antibodies

    Characterization of Monoclonal Antibodies Specific for a High Molecular Weight Glycoprotein Secreted from Activated Human Blood Platelets

    Characterization of a Monoclonal Antibody Directed Against A Platelet Alpha-Granule Glycoprotein

    B.6. Tumors

    B.6.1. Breast Tumors

    Enhanced Expression of Surface Tumor Associated Antigens on Human Breast and Colon Tumor Cells by Recombinant Human Interferon

    Human and Murine Monoclonal Antibodies Against Human Mammary Carcinoma Cells

    Modification of the Regulation of Interleukin 2 (IL2) in Human Breast Cancer

    Comparison Between Glytolytic Enzyme Activities in Benign and Malignant Breast Tumors

    Value of Determination of CEA and PHI Levels in Breast Tissue: Correlation with Steroid Receptors

    Clinical Usefulness of the Simultaneous Determination of Tumor Markers and Steriod Receptors in Breast Cancer

    Value of Biochemical Examination of Cyst Fluid in the Classification of Mammary Fibrocystic Disease

    B.6.2. Lymphoid Malignancies

    Regulation of Cortisol Metabolism by Lymphocytes

    Galactosyltransferase Activity/Sialyltransferase Activity as an Index of Membrane Glycosylation of Lymphocyte Subpopulations

    Monitoring of a Human B Cell Lymphoma with Monoclonal Anti-Idiotype Antibodies

    Monoclonal Antibody (HD 39) Reactive with a Human B Lymphocyte Specific Antigen Expressed on Late Stages of B Cell Maturation

    Interrelationship of Hairy Cell Leukemia and Phorbol Ester Induced B-Cell "Lymphocytes" as Demonstrated by Monoclonal Antibodies

    Identification and Monitoring of Terminal Deoxynucleotidyl Transferase Positive Cells in the Cerebrospinal Fluid of Children with Lymphoid Malignancies

    BLA: A Glycolipid Antigen Associated with Burkitt's Lymphoma Cells

    Expression of B Cell Markers in Centroblastic/Centrocytic Non-Hodgkins Lymphomas

    Binding and Endocytosis of Rat Monoclonal Anti-Immunoglobulin Antibodies by Human Lymphoma Cells

    A Monoclonal Antibody (HAN-PC1), Reacting with a Maturation Antigen on Plasma Cells

    Monoclonal Antibody BI-3C5 Reacts Specifically with M1/M2-Acute Myeloid Leukaemias and Most Non-B Non-T Acute Lymphocytic Leukaemias, and Recognises a Small Population of Immature Cells in Normal Bone

    B.6.3. Various Tumors

    Altered Helper-T, Suppressor-T Cell Representation in Those with Advanced Stage Prostatic Cancer

    Oral Adjuvants Which Enhance or Replace T Cells for IgA Responses

    Detection of Antibodies Against Herpes Simplex Viruses in Sera of Patients with Cervical Cancer by Using the Western Blotting Technique

    Definition by Murine Monoclonal Antibodies of Cell Surface Structures Exertin g Fc- and C3bi-Receptor Activity in Vivo

    Increased Urokinase-Type Plasminogen Activator in Tumors of the Urogenital Tract

    Radioimmunoassay and Binding Capacity of Sex Hormone Binding Globulin (SHBG) in Patients with Gynaecological Cancer

    Isolation of Hepatitis B Surface Antigen from Human Plasma by Affinity Chromatography Using Monoclonal Antibody

    Competitive Screening and Determination of the Affinity Constants for the Characterization of Monoclonal Antibodies Against Human Chorionic Somatomammotropin

    Flow Cytometric Analysis of Mixed Cell Populations Using Antibodies to Cytokeratin

    Isolation and Characterization of Prostatic Tumor Effusions

    Monoclonal Antibodies for Purification and RIA of Natural and Recombinant Human Interferon-α, -β and-γ

    Isolation and Characterization of Anti-Z-DNA Antibodies in Sera of Patients with SLE

    Section C. Advances in Separation of Protides

    C.1. Detection by Color Labelling

    High Sensitivity Analysis of Amino Acids, Peptides and Proteins by Color Labelling Techniques

    C.2. 2-D E.P.

    New Proteins Unique to Cerebrospinal Fluid (CSF) Identified by Two-Dimensional Electrophoresis (2DE)

    A Simple Method for Quantitative Analysis of Protein in Spots Separated by Two-Dimensional Polyacrylamide Gel Electrophoresis and its Applications

    Two-Dimensional Affinity Electrophoresis: Its Application to Separation of Individual Immunoglobulins from Heterogeneous Antibodies

    Analysis of Genetic Muscle Disorders by Two-Dimensional Electrophoresis

    Multidimensional Electrophoresis of Proteins

    C.3. E.P.

    A Continuous Elution Procedure for Agarose Suspension Electrophoresis of Neuropeptides

    Electrophoretic Heterogeneity of Human Prolactin in Pregnancy Urine

    Nitrocellulose Blotting of CSF and Urine Protides

    Immunological Characterization of Creatine Kinase MM Sub-Bands After Isoelectric Focusing and Electroblotting

    pH Gradient Manipulation in Isoelectric Focusing Using Zwitterionic Buffers

    Presumptive Identification of Human Hemoglobin Variants by Combined Electrophoresis

    Spiroplasma citri Membrane Protein Analysis by Sodium Deoxycholate-Crossed Immunoelectrophoresis with a Sieving First Directional Gel

    Concentration of Proteins by Application of the Kohlrausch Regulation Function

    An Analysis of the Microheterogeneity of Pig Fumarase

    Two Dimensional Anionic-Cationic Detergents Polyacrylamide Gel Electrophoresis: A New Strategy in the Separation of Membrane Proteins

    Methods for Identification and Quantification of Alkaline Phosphatase Isoenzymes in Human Serum

    Studies on LMG (Low-Mobility-Group), A Class of Chromosomal Proteins from Calf Thymus

    C.4. H.P.L.C

    The Use of HPLC Procedures in the Study of Human Hemoglobin Variants

    Use of HPLC Techniques and Monoclonal Antibodies in the Study of Lemur Hemoglobins

    Use of Mini Column in RP-HPLC for the Complete Primary Structure Determination of Proteins

    The Use of Ion Exchange and Reversed-Phase HPLC for the Isolation and Characterization of Interferon Gamma

    High-Performance Liquid Chromatography of Detergent Extracted Herpes Simplex Type 1 Virions

    Purification of Chromosomal HMG Proteins by HPLC Characterization of Two New Species: HMG O and HMG X

    High-Performance Liquid Chromatography of Viral Proteins as a Tool in the Design of Synthetic Vaccines

    Isolation of an Immunoreactive Thymosin Fraction by Chromatofocusing and High-Performance Liquid Chromatography

    Peptide Separation Using Guanidine-HCL During Reverse-Phase High Performance Liquid Chromatography

    Isolation of Peptide Fragments of the Pyrimidine Biosynthetic Complex by HPLC

    High Performance Hydrophobic Interaction Chromatography of Proteins: Application to the Purification of Black Mamba Venom

    Application of HPLC to the Separation of Glycoproteins from Endothelial Cell Culture Medium

    Reverse-Phase High-Performance Liquid Chromatography of Virus Proteins and Other Hydrophobic Proteins

    Fast Protein Liquid Chromatography of IgA Fragments

    Determination of Thymosin β4 in Cultured Cell Lines

    Separation of Ceresbrospinal Fluid Proteins by Anion Exchange and Reverse Phase Chromatography

    FPLC for the Isolation of a New Intermediate in the Reaction of the Fifth (C5) and Sixth (C6) Component of Complement

    C.5. Affinity Chromatography

    Na-Channel Purification by Ion-Exchange and Affinity Chromatography

    Chromatography of Plasma Proteins on Immobilized Cibacron Blue, Procion® Red and Procion® Green: Use for Purification of Alpha 1-Proteinase Inhibitor

    Purification of Human Epidermal Growth Factor-Urogastrone by Immunoaffinity Chromatography

    Combined Pseudo-Ligand Affinity Chromatography as a General Method for Plasma Protein Purification

    Purification of Glucocorticoid Receptor from Rabbit Liver by Steroid Affinity Chromatography

    Characterization of Steroid Receptors Using Ion Exchange Fast Protein Liquid Chromatography (FPLC)

    C.6. Various Fractions

    Binding of Rat Liver Glucocorticoid Receptor by Heparin Ultrogel

    Current Advances in the Purification and Characterization of Progesterone Receptors

    Purification and Characterization of the Rat Kidney and Cerebellum 28K Vitamin D-Dependent Calcium Binding Proteins

    Isolation of C1Q Component of Human Complement — New Three-Step Purification Procedure

    Two Point Turbidimetric Alcian Blue Test for Quantitative Estimation of Glycosaminoglycans (GAG) in Human Urine

    5'-Nucleotidase from Bovine Liver Plasma Membrane: Effect of Cations and Chelating Agents

    Antibodies Against Bovine Liver Plasma Membrane 5'-Nucleotidase

    Hemoglobin Beirut (β126 val → ala [H4]) Synthesis in Erythroid Cultures

    Chemotropic Neomorphogenesis of Hemodynamically Active Hypervascular Patterns in Hare Skin by a Monocytic Polypeptide Mediator for Blood Vessel Growth (Monocyto-Angiotropin) Promotes Tissue Hyperfunction (Hair Growth)

    Leukocytic Polypeptide Wound Hormones: Phenotype-Selective Chemorecruitment of Bone Marrow Leukocytes into Blood Circulation (Leukocytosis-Leukopenia-Leftward Shift Reactions)

    Acute Cardiovascular Effects of Purified Classical Anaphylatoxin in Guinea Pigs and Rabbits: Systemic Activities of a Humoral Mediator of Inflammation Relate to Heart Muscle Dysfunction

    Clinical Assessment of Neutropenia and of Reserve Pools of Leukocyte Phenotypes of Distinct Granulopoietic Maturation Stage in Bone Marrow of Humans by Purified Humoral and Cellular Polypeptide Effectors for Leukocytosis and Leftward Shift Reactions

    Author Index

    Subject Index

Product details

  • No. of pages: 1236
  • Language: English
  • Copyright: © Pergamon 1985
  • Published: January 1, 1985
  • Imprint: Pergamon
  • eBook ISBN: 9781483279664

About the Editor

H. Peeters

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