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Protides of the BIological Fluids - 1st Edition - ISBN: 9780080298153, 9781483279640

Protides of the BIological Fluids, Volume 30

1st Edition

Proceedings of the Thirtieth Colloquium, 1982

Editor: H. Peeters
eBook ISBN: 9781483279640
Imprint: Pergamon
Published Date: 1st January 1983
Page Count: 800
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Table of Contents



XXXth Anniversary Lecture

30 Years of Protides — An Anniversary and Challenge

The Eleventh Arne Tiselius Memorial Lecture

High Performance Liquid Chromatography on Matrices of Agarose

Section A. Neuroproteins

A.1. Glial Markers

A.1.1. S 100

Chemical Structure and Molecular Evolution of S-100 Proteins

Some Properties of S-100 Protein in Solution

The Relationship Between S-100 Protein and Specific Synaptosomal Components

Identification and Characterization of Sulfhydryl-Containing Proteolytic Fragments Involved in the Ca-Induced Conformational Change of Beef Brain S 100

The S-100 Antigen in Non-Neural Tissues

Immunohistochemical Localization of Nervous Tissue Proteins, S-100, GFAP and Astroprotein in Extraneural Tissues

A.1.2. GFA

Brain Specific Protein. Some General Remarks

A.2. Neuronal Markers

A.2.1. Enolase

Neuron-Specific Enolase (NSE) in Biological Fluids: A Marker of Neuronal Lesions

Rodent Enolase Cerebral Isozymes as Differentiation Markers

Neuron Specific Enolase in Biological Fluids: A Probe for Neuronal and Neuroendocrine Pathology

Neuron Specific Enolase — An Indicator of Brain Damage Due to Hypoxia in Newborns and Adults

A.2.2. D2

Characterization of the D2-Cell Adhesion Molecule

A Neuronal Adhesion Molecule in the Central Nervous System: The D2-Protein

Purification of the Glycoprotein D2 From Fetal and Adult Human Brain

Developmental Study of Chick D2

A.3. Synapse Specific Protein

Characterization of Antigens of the Cholinergic Synapses in the Electric Ray Torpedo Marmorata

A Possible Physiological Role of Acetylcholine Receptor on Mouse Thymocytes

A.4. Myelin Basic Protein

Oligodendroglial, Astroglial and Glial Tumor-Associated Antigens Defined by Monoclonal Antibodies

Posttranslational Modifications of Myelin Basic Protein

“Myelin-Like” or Pre-Myelin Structures in Cultures of Dissociated Brain Cells from 14-15 Day-Old Embryonic Mice

Immunohistochemistry and RIA Determination of MBP as Complementary Tools to Study Myelin Formation in the CNS and PNS of Mice

Immunologic Reactivity of Myelin Basic Protein (BP) in Inbred Guinea Pigs

Myelin Basic Protein in the MLD Mutant Mouse During Development

A.5. Regional Markers

A.5.1. Galactocerebrosides

Galactosyl- and Sulfogalactosylceramide as Central Nervous Tissue Markers

Purification of a Membrane Glycoprotein from Fetal Human Brain

Characterization of Hyaluronectin in the Nervous System

Two Markers for Brain Tumors: Glial Fibrillary Acidic Protein (GFA) and Hyaluronectin (HN)

Accessibility of Sulfhydryl Groups of Myelin Folch-Pi Protein

A.5.2. Carbonic Anhydrase

Immunochemical and Immunohistological Study of Carbonic Anhydrase in the Rat CNS

A.6. Neurofilaments

In Vivo and In Vitro Studies of Neurofilament Protein Assembly

A.7. Protein Metabolism

Brain Protein Responses to Opiates

Isolation and Partial Biochemical Characterization of a Novel Polypeptide from Human CNS and Liver

Identification of Nuclear Protein Antigens of Rat Brain

Low Angle X-Ray Diffraction Studies on Myelin Isolated from Normal Human White Matter and from Patients Dying with Multiple Sclerosis

Central Nervous System Immunoglobulins (Ig) in Adrenoleukodystrophy (ALD)

A.8. EAE (Experimental Allergic Encephalomyelitis)

Effect of Human Fetal Alpha-Fetoprotein on Experimental Allergic Encephalomyelitis and the Immune Response to Myelin Antigens

Immune Regulation of Autoimmune Encephalomyelitis

In Vivo Demyelinating Activity of Sera from Animals with Chronic Relapsing Experimental Allergic Encephalomyelitis (Cr-EAE)

“Fail-Safe” Determinants for Immuno-Regulating Autoimmune Demyelinating Diseases

Serum Antibodies Against Central Nervous System (CNS) Antigens in Chronic Relapsing Experimental Allergic Encephalomyelitis (CrEAE)

“Recovery Associated” Suppressor Cells in Experimental Allergic Encephalomyelitis (EAE): Replacement of their Activity In Vivo by Cell Free Extracts

Demonstration of Serum IgG Antibodies Against Myelin During the Course of Relapsing Experimental Allergic Encephalomyelitis (r-EAE) in Guinea Pigs

A.9. Determinations in CSF

Studies on the S-100 Antigen in Cerebrospinal Fluid of Neurological Patients

Determination of α-Albumin (GFA) in Cerebrospinal Fluid

Clinical Applications of Human Brain-Specific Protein Measurements

The Determination of Trace Proteins and Anti-Myelin Basic Protein Antibodies in the Cerebrospinal Fluid by Particle Counting Immunoassay (PACIA)

Radioimmunological Determination of Myelin Basic Protein in the CSF of Neurological Patients

An Enzyme Immuno Assay for the Detection of Basic Myelin Protein (BMP) and Antibodies to BMP in CSF

CSF Autoantibodies Detect Brain Antigens

Galactocerebroside Antibodies in Serum and Cerebrospinal Fluid of Multiple Sclerosis and Brain Tumour Patients

Determination of α1-Acid Glycoprotein in Human Cerebrospinal Fluid (CSF) by an Immunonephelometric Assay

Determination of Methionine-Enkephalin and Leucine-Enkephalin in Human Cerebrospinal Fluid (CSF)

Determination of Neurotensin in Human Cerebrospinal Fluid (CSF)

Polyacrylamide Gel Electrophoresis Technique for the Separation of High Molecular Weight Proteins in Native Cerebrospinal Fluid and Serum

The Correlation of the IgG Patterns of CSF Determined by Isoelectric Focusing with Clinical and Biochemical Parameters of Multiple Sclerosis

Direct Tissue Isoelectric Focusing of Nervous System and Muscle Sections for Detection of IgG Patterns

Section B. Monoclonal Antibodies

B.1. Hybridoma Technology

A New Human Hybridoma System (LICR-LON-HMy2) and its use in the Production of Human Monoclonal Antibodies

Production of Human Antibodies of Predefined Specificity In Vitro

Hybridomas Grown in Serum-Free Medium

Strategy to Obtain Monoclonal Anti-Idiotype Antibodies

Monoclonal Antibodies Against Carbohydrate Antigens

Multivalent Antibody RIA: A New Assay Using Only Antigen Screening Hybridoma Cultures

A Radio-Immunobinding Method on Glutaraldehyde-Fixed Cells for the Screening of Hybridoma Supernatants

Specificity Screening of Monoclonal Antibodies Obtained After Immunization with A Human Myeloma Cell Line (ARH-77)

Monoclonal Antibodies and Precipitin Reactions

Determination of Affinity and Concentration of Monoclonal Antibodies in Ascitic Fluids

Monoclonal Murine Antibodies Reactive with Cell Nuclei

B.2. Oncology

B.2.1. Diagnosis

Monoclonal Antibodies with Dual Antigen Specificity

Monoclonal Antibodies to Cell Surface Antigens of Mouse Bladder Carcinomas

Structural Studies of Human Melanoma-Associated Antigen p97

Cell Surface Antigens of Human Melanoma

A Double Determinant Immunoassay (DDIA) With Monoclonal Antibodies to a Human High Molecular Weight Melanoma Associated Antigen (HMW-MAA)

Immunological Studies on Membrane Associated Antigens from Renal Adenocarcinoma and Human Kidney Cortex Using Poly- and Monoclonal Antibodies

Malignin, Anti-Malignin Antibody and SCANTAG

B.2.2. Therapy

Results of Early Trials Using Murine Monoclonal Antibodies as Anti-Cancer Therapy

High Efficiency of Immunotoxin Potentiated by Lysosomotropic Amines to Specifically Kill Tumor Cells

In Vivo Effect of the Conjugate of Monoclonal Antibody with Cytotoxic Agent on the Tumor Growth of Human Melanoma

Monoclonal Antibodies Against Human Gastric Carcinomas

Monoclonal Antibodies for Targeted Therapy with Vindesine

Diagnostic and Therapeutic Applications of a Monoclonal Antibody Against Human Osteogenic Sarcoma

B.3. Coagulation

Identification of Human Blood Platelet Membrane Congenital Defects by the Use of Monoclonal Antibodies

Evidence that the Monoclonal Antibody AN 51 is Directed Against Platelet Membrane Glycoprotein Ib

Monoclonal Antibodies to Prothrombin: A Study of their Specificities for the Different Prothrombin Activation Products

Monoclonal Antibodies for Domains which are Differentially Expressed in Fibrinogen, in Fibrinogen Degradation Products or in Fibrin Degradation Products

Affinity Depletion and Affinity Purification of Human Factor IX by Monoclonal Antibodies

Monoclonal Antibodies in Analysis of Trypsin Digested Proteolytic Fragments of Human Plasma Fibronectin

B.4. Leukemia

Monoclonal Antibodies Differentiating A Subtype of Leukemias

Clinical Utility of Monoclonal Antibodies in the Phenotyping of Acute and Chronic Lymphocytic Leukemia

Leukemia Diagnosis with Monoclonal Antibodies

Monoclonal Antibodies to Human Myeloid Associated Antigens

Characterization of a Murine Monoclonal Antibody Specific for Early Lymphohemopoietic Cells

B.5. T Cells

Unexpected Specificities of Monoclonal Antibodies

In Situ Analysis of Mononuclear Cell Infiltrate in Liver Biopsies of Patients with Orthotopic Liver Transplantation

T Lymphocytes in the Human Fetus, Studied with Monoclonal Antibodies

A Monoclonal Antibody Directed Against HLA-DR Molecules Inhibits the Interleukin-2-Dependent T Cell Proliferation in Humans

Surface Phenotype of Helper and Suppressor Human T Cell Clones

T-Lymphocyte Subpopulations in Peripheral Blood of Long-Survivors with Hodgkin’s Disease Off-Therapy: Determinations by Means of Monoclonal Antibodies

Human Blood T-Cell Subpopulations Identified by Monoclonal Antibodies in Ageing

Mouse Hybridoma Lines Specific for Genetic Markers of the Rabbit Immunoglobulins (Allotypes)

Monoclonal Anti-Allotype Antibodies as Probes for the Rabbit T-Cell Receptor

Monoclonal Antibodies Against Ia +, Antigen Specific T Cell Factors

B.6. B Cells

Monoclonal Anti-Immunoglobulin Reagents in the Detection of Immunoglobulin on the Surface of Normal and Neoplastic B Lymphocytes

Analysis of Human B Cell Membrane Antigens by Means of Monoclonal Antibodies

B.7. Interferon

Monoclonal Antibodies to Human Leukocyte and Fibroblast Interferon

Monoclonal Antibodies Against Human Leucocyte Interferons for the Definition of Subclasses and their Affinity Purification

B.8. Infectious Diseases

Monoclonal Antibodies to Hepatitis Antigens as Diagnostic Tools

Affinity Purification of Hepatitis B Surface Antigen Using RF-HBs-1 Monoclonal Antibody

A Serological Test for Tuberculosis Based on Murine Monoclonal Antibodies

Monoclonal Antibodies to Poliovirus and its Capsid Proteins

Production and Use of Monoclonal Antibodies to Polioviruses

Monoclonal Antibodies and Diagnosis of Schistosomiasis in Man

B.9. Neuroproteins

Monoclonal Antibodies to Myelin Basic Protein

Monoclonal Antibodies as Probes for Neural Cell-Surface Glycoproteins

Ultrastructural Immunohistochemical Study of Nerve Cell Surface Antigens with Monoclonal Antibodies

Monoclonal Antibodies Against Glial Fibrillary Acidic Protein

B.10. Diagnostic Reagents

Monoclonal Antibodies in Simultaneous Marking ELISA Systems

Advantages and Disadvantages in the Application of Monoclonal Antibodies as Tools in Clinical Diagnosis — Monoclonal Anti-HCG in Immunoassay Systems

Production and Characterization of Monoclonal Antibodies Specific for Parathormone

Immunogenic and Antigenic Epitopes of Immunoglobulins VI. Application of Monoclonal Antibodies to Human IgG Sub-Classes in Quantitative Assays

Monoclonal Antibodies Against M1 Antigen, A Fucomucin of the Human Gastrointestinal Mucosa

Study of the Dissociation Constant of Four Monoclonal Hybridoma Antibodies Specific to Human β2 Microglobulin. Effect of 2 Mercapto-ethanol on their Activity

Two Monoclonal Antibodies Against Human Cardiac Myosins

Development of a Screening System for the Detection of Somatic Mutations: Detection of Monoclonal Antibodies Specific for Hemoglobin Determinants

Section C. Separation Methods

C.1. Electrophoretic Techniques

Methods of Two-Dimensional Gel Electrophoresis for the Clinical Laboratory

The Demonstration of Human Brain-Specific Soluble Proteins Using High Resolution Two-Dimensional Polyacrylamide Gel Electrophoresis

Brain Proteins in Soluble and Insoluble Fractions Analyzed by Micro Two-Dimensional Electrophoresis

Demonstration of Prealbumin, Apolipoprotein A I and 7-S-Beta1-Globulin from Human Blood by Double One-Dimensional Electrophoresis

Immobilized pH Gradients for Isoelectric Focusing. I. Theory and Methodology

Isoelectric Focusing in Immobilized pH Gradients. II. Application to Hemoglobin Analysis

Isoelectric Focusing in Immobilized pH-Gradients. III. Application to Two-Dimensional Separations

C.2. Chromatofocusing

The Separation of Serum Proteins by Chromatofocusing

Chromatofocusing Applied to the Separation of Human Hemoglobins and Globin Chains

Basic Principles Used in the Selection of Monobeads Ion Exchangers for the Separation of Biopolymers

Physicochemical Considerations in the Use of Monobeads for the Separation of Biological Molecules

Fast Protein Separations on Mono O, Mono S and Mono P

Recommendations for Obtaining Optimal Separations on Monobead Ion Exchangers

Sample Loading Capacity on Monobeads Ion Exchangers

Two Dimensional Analysis of Human Sera By Chromatofocusing on Mono P Followed by Fused Rocket Immunoelectrophoresis

Analysis of Human Blood Plasma by Chromatofocusing on Mono P Followed by Pore Gradient Gel Electrophoresis

Chromatofocusing of Desialylated Human Transferrin on Mono P

C.3. Affinity Chromatography

Immunoaffinity Chromatography with Monoclonal Antibodies

Separation of Copper-Binding Peptides From Human Ceruloplasmin by Metal Chelate Affinity Chromatography and HPLC

Chromatography of Serum Proteins on Immobilized Cibacron Blue F3GA

Chromatography of Pig Pituitary Extracts on Inactivated Kallikrein-Sepharose

Purification of Human Lymphocyte Ecto-5′Nucleotidase in Order to Prepare a Monoclonal Antibody


High Pressure Liquid Chromatography Using Ampholyte Displacement (HPADC)

High Speed Gel Filtration as a Tool to Optimize Protein Fragmentation

Reverse-Phase HPLC of Proteins Using Large Pore Silica Based Supports

Assessment Study on the Use of the Low Angle Laser Light Scattering Technique in Combination with the High Performance Porous Silica Gel Chromatography

Combined UV- and Alkaline Hydrolysis/o-Phthal Aldehyde Reaction Detection in Preparative HPLC of Proteins and Peptides with Samples From Complex Biological Fluids

The Use of Size Exclusion and C18 Reverse Phase HPLC Columns for Separating the Component Polypeptides of Ia Antigen Membrane Proteins

Isolation and Characterization of Intracellular Ca2+-Binding Proteins by High Performance Liquid Chromatography

Evaluation of Commercial RP-HPLC Columns for the Separation of Peptides from Porcine Cardiac Myosin Light Chain L27

The Optimization of RP-HPLC of Proteins with Large Pore-Size Short Alkylchain-Bonded Silica (Ultrapore RPSC) and its Application to Epidermal Growth Factor

High Performance Liquid Chromatography of Peptides on Macroreticular Polystyrene Resins

HPLC Separation of Axonally Transported Proteins in Sciatic Nerves

Immune Complex Analysis by a Combination of High Pressure Liquid Gel Permeation Chromatography (HPLC GPC) and Enzyme Immunoassay (ELISA)

Isolation of Thymosin β8 and β9 From Calf Thymus

Author Index

Subject Index


Protides of the Biological Fluids: Proceedings of the 30th Colloquium, 1982 is a collection of manuscripts presented at the 30th Colloquium, held in Brussels, Belgium in 1982.

This text is organized into three sections encompassing 160 chapters. The first section highlights the mechanisms of the normal and abnormal mental function through evaluation of neuroproteins or the so-called “think-proteins”. This section describes the manifestations, clinical features, diagnosis, and therapeutic options of nervous system diseases. The second section tackles monoclonal proteins as a reagent to detect antigens. This section deals with the significant progress in cancer therapy and the binding of cytotoxic drugs to monoclonal antibodies. The third section considers the potential of isodalt electrophoresis and high-pressure liquid chromatography, with particular emphasis on their application to protein analysis in biological fluids.

This book will be of great value to biochemists, clinical chemists, and clinicians.


No. of pages:
© Pergamon 1983
1st January 1983
eBook ISBN:

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About the Editor

H. Peeters